Construct: sgRNA BRDN0001146828
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGCTAGAACACGTGTCAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRSK2 (9024)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78025
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
1440812 |
+ |
BRSK2 |
NNNCTAGAACACGTGTCAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
2762299 |
+ |
SRRM2 |
NNNCTAGAACACCTGCCAGG |
NGG |
2 |
0.2807 |
Tier I |
| 3 |
NC_000019.10 |
19 |
55386196 |
- |
RPL28 |
NNNCTAGAACTCTTGTCAGG |
NGG |
2 |
0.0923 |
Tier II |
| 4 |
NC_000007.14 |
7 |
149938036 |
- |
ACTR3C |
NNNCTAGAACACGGGTCGGG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000023.11 |
X |
147258607 |
+ |
LOC105373347 |
NNNCTAGATCACGTGTCTGG |
NGG |
2 |
0.3 |
Tier III |
| 6 |
NC_000023.11 |
X |
147260382 |
+ |
LOC105373347 |
NNNCTAGATCACGTGTCTGG |
NGG |
2 |
0.3 |
Tier III |
| 7 |
NC_000007.14 |
7 |
149938036 |
- |
LOC124901772 |
NNNCTAGAACACGGGTCGGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
141984418 |
+ |
Brsk2 |
NNNCTAGAACATGTGTCTGG |
NGG |
2 |
0.2692 |
Tier I |
| 2 |
NC_000078.6 |
12 |
75818073 |
- |
Gm40447 |
NNNCTAGAACACTTTTCAGG |
NGG |
2 |
0.0429 |
Tier I |
| 3 |
NC_000075.6 |
9 |
117071148 |
- |
Rbms3 |
NNNCTATAACACGTGTCAGG |
NGG |
1 |
0.5714 |
Tier II |
| 4 |
NC_000080.6 |
14 |
77970026 |
- |
Epsti1 |
NNNCTAGAAAACGTGTCAGC |
NGG |
2 |
0.3714 |
Tier II |
| 5 |
NC_000083.6 |
17 |
32348923 |
+ |
Akap8l |
NNNCTAGAACCCATGTCAGG |
NGG |
2 |
0.3692 |
Tier II |
| 6 |
NC_000070.6 |
4 |
46495794 |
+ |
Nans |
NNNGTAGGACACGTGTCAGG |
NGG |
2 |
0.3667 |
Tier II |
| 7 |
NC_000081.6 |
15 |
82036509 |
- |
Xrcc6 |
NNNCTAGAAAACCTGTCAGG |
NGG |
2 |
0.3649 |
Tier II |
| 8 |
NC_000078.6 |
12 |
34895468 |
- |
Hdac9 |
NNNCTAGGACACCTGTCAGG |
NGG |
2 |
0.3088 |
Tier II |
| 9 |
NC_000077.6 |
11 |
118902498 |
- |
Rbfox3 |
NNNCTAGAACACATGTCAGG |
NAG |
2 |
0.2393 |
Tier II |
| 10 |
NC_000073.6 |
7 |
118763937 |
- |
Vps35l |
NNNCAAGAACACGTGTAAGG |
NGG |
2 |
0.2333 |
Tier II |
| 11 |
NC_000082.6 |
16 |
97613398 |
- |
Tmprss2 |
NNNCTAGAACACCTGTAAGG |
NGG |
2 |
0.1965 |
Tier II |
| 12 |
NC_000073.6 |
7 |
16035736 |
- |
Bicra |
NNNCTAGCACCCGTGTCAGG |
NGG |
2 |
0.1714 |
Tier II |
| 13 |
NC_000070.6 |
4 |
106435312 |
- |
Usp24 |
NNNCTAGCACACGTGTCAGG |
NAG |
2 |
0.1111 |
Tier II |
| 14 |
NC_000079.6 |
13 |
112340977 |
+ |
Ankrd55 |
NNNCCAGAACACGTGTCAGG |
NTG |
2 |
0.039 |
Tier II |
| 15 |
NC_000069.6 |
3 |
108957029 |
- |
Henmt1 |
NNNCTAGAACCCGTGTCAGG |
NGA |
2 |
0.0278 |
Tier II |
| 16 |
NC_000085.6 |
19 |
61112461 |
- |
Zfp950 |
NNNCAAGAACACGTGTCAGG |
NGT |
2 |
0.0081 |
Tier II |
| 17 |
NC_000075.6 |
9 |
28302102 |
- |
Opcml |
NNNCTAGAACACGTGTGAGG |
NGC |
2 |
0.0013 |
Tier II |
| 18 |
NC_000068.7 |
2 |
180564427 |
- |
Gm30144 |
NNNCTAGAAAACATGTCAGG |
NGG |
2 |
0.8 |
Tier III |
| 19 |
NC_000075.6 |
9 |
61766833 |
+ |
Gm34828 |
NNNCCAGAACCCGTGTCAGG |
NGG |
2 |
0.4 |
Tier III |
Other clones with same target sequence:
(none)
