Construct: sgRNA BRDN0001146828

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGCTAGAACACGTGTCAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRSK2 (9024)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78025

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 1440812 + BRSK2 NNNCTAGAACACGTGTCAGG NGG 0 1.0 Tier I
2 NC_000016.10 16 2762299 + SRRM2 NNNCTAGAACACCTGCCAGG NGG 2 0.2807 Tier I
3 NC_000019.10 19 55386196 - RPL28 NNNCTAGAACTCTTGTCAGG NGG 2 0.0923 Tier II
4 NC_000007.14 7 149938036 - ACTR3C NNNCTAGAACACGGGTCGGG NGG 2 0.0 Tier II
5 NC_000023.11 X 147258607 + LOC105373347 NNNCTAGATCACGTGTCTGG NGG 2 0.3 Tier III
6 NC_000023.11 X 147260382 + LOC105373347 NNNCTAGATCACGTGTCTGG NGG 2 0.3 Tier III
7 NC_000007.14 7 149938036 - LOC124901772 NNNCTAGAACACGGGTCGGG NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 141984418 + Brsk2 NNNCTAGAACATGTGTCTGG NGG 2 0.2692 Tier I
2 NC_000078.6 12 75818073 - Gm40447 NNNCTAGAACACTTTTCAGG NGG 2 0.0429 Tier I
3 NC_000075.6 9 117071148 - Rbms3 NNNCTATAACACGTGTCAGG NGG 1 0.5714 Tier II
4 NC_000080.6 14 77970026 - Epsti1 NNNCTAGAAAACGTGTCAGC NGG 2 0.3714 Tier II
5 NC_000083.6 17 32348923 + Akap8l NNNCTAGAACCCATGTCAGG NGG 2 0.3692 Tier II
6 NC_000070.6 4 46495794 + Nans NNNGTAGGACACGTGTCAGG NGG 2 0.3667 Tier II
7 NC_000081.6 15 82036509 - Xrcc6 NNNCTAGAAAACCTGTCAGG NGG 2 0.3649 Tier II
8 NC_000078.6 12 34895468 - Hdac9 NNNCTAGGACACCTGTCAGG NGG 2 0.3088 Tier II
9 NC_000077.6 11 118902498 - Rbfox3 NNNCTAGAACACATGTCAGG NAG 2 0.2393 Tier II
10 NC_000073.6 7 118763937 - Vps35l NNNCAAGAACACGTGTAAGG NGG 2 0.2333 Tier II
11 NC_000082.6 16 97613398 - Tmprss2 NNNCTAGAACACCTGTAAGG NGG 2 0.1965 Tier II
12 NC_000073.6 7 16035736 - Bicra NNNCTAGCACCCGTGTCAGG NGG 2 0.1714 Tier II
13 NC_000070.6 4 106435312 - Usp24 NNNCTAGCACACGTGTCAGG NAG 2 0.1111 Tier II
14 NC_000079.6 13 112340977 + Ankrd55 NNNCCAGAACACGTGTCAGG NTG 2 0.039 Tier II
15 NC_000069.6 3 108957029 - Henmt1 NNNCTAGAACCCGTGTCAGG NGA 2 0.0278 Tier II
16 NC_000085.6 19 61112461 - Zfp950 NNNCAAGAACACGTGTCAGG NGT 2 0.0081 Tier II
17 NC_000075.6 9 28302102 - Opcml NNNCTAGAACACGTGTGAGG NGC 2 0.0013 Tier II
18 NC_000068.7 2 180564427 - Gm30144 NNNCTAGAAAACATGTCAGG NGG 2 0.8 Tier III
19 NC_000075.6 9 61766833 + Gm34828 NNNCCAGAACCCGTGTCAGG NGG 2 0.4 Tier III
Download CSV

Other clones with same target sequence:

(none)

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