Construct: sgRNA BRDN0001146830
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTGGCATATTGGCCCAGAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80231
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
54682640 |
+ |
KIT |
NNNAGCATATTGGCCAAGAC |
NGG |
2 |
0.9 |
Tier II |
| 2 |
NC_000023.11 |
X |
13574922 |
+ |
EGFL6 |
NNNAGCATATTGGCCCAAAC |
NGG |
2 |
0.6231 |
Tier II |
| 3 |
NC_000016.10 |
16 |
90014813 |
- |
DBNDD1 |
NNNGCCATGTTGGCCCAGAC |
NGG |
2 |
0.5238 |
Tier II |
| 4 |
NC_000020.11 |
20 |
5066072 |
+ |
TMEM230 |
NNNCCCATATTGGCCCAGAC |
NGG |
2 |
0.416 |
Tier II |
| 5 |
NC_000005.10 |
5 |
1052880 |
- |
SLC12A7 |
NNNGGCGTATTGGCACAGAC |
NGG |
2 |
0.1569 |
Tier II |
| 6 |
NC_000004.12 |
4 |
56668650 |
- |
HOPX |
NNNGGCATAATGGCTCAGAC |
NGG |
2 |
0.0571 |
Tier II |
| 7 |
NC_000013.11 |
13 |
23864021 |
- |
MIPEP |
NNNGGCATATTGGACCAGAC |
NTG |
2 |
0.0136 |
Tier II |
| 8 |
NC_000023.11 |
X |
13574922 |
+ |
RPL30P15 |
NNNAGCATATTGGCCCAAAC |
NGG |
2 |
0.6231 |
Tier III |
| 9 |
NC_000001.11 |
1 |
16160069 |
+ |
EPHA2-AS1 |
NNNGGCATTCTGGCCCAGAC |
NGG |
2 |
0.32 |
Tier III |
| 10 |
NC_000002.12 |
2 |
47318545 |
- |
EPCAM-DT |
NNNGGCATGTTGGCCCAGGC |
NGG |
2 |
0.25 |
Tier III |
| 11 |
NC_000011.10 |
11 |
88346368 |
- |
LOC101929174 |
NNNGGCATATTGGACCAGAG |
NGG |
2 |
0.0206 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
152736254 |
+ |
Pigk |
NNNGGCACATGGGCCCAGAC |
NGG |
2 |
0.4 |
Tier II |
| 2 |
NC_000079.6 |
13 |
83534143 |
+ |
Mef2c |
NNNGGCTTATTGGTCCAGAC |
NGG |
2 |
0.3208 |
Tier II |
| 3 |
NC_000070.6 |
4 |
9156743 |
- |
Clvs1 |
NNNGGCATATTGGTCCAGGC |
NGG |
2 |
0.275 |
Tier II |
| 4 |
NC_000075.6 |
9 |
94941584 |
- |
Slc9a9 |
NNNGGCATATTGCCCCAGTC |
NGG |
2 |
0.2267 |
Tier II |
| 5 |
NC_000082.6 |
16 |
24509971 |
+ |
Lpp |
NNNGGCATGTTGGCTCAGAC |
NGG |
2 |
0.0444 |
Tier II |
| 6 |
NC_000073.6 |
7 |
143386693 |
+ |
Kcnq1 |
NNNGGCATATTTGCCCAGAC |
NGC |
2 |
0.0085 |
Tier II |
| 7 |
NC_000077.6 |
11 |
52663528 |
+ |
Gm30366 |
NNNGGTATATTGGCCCAAAC |
NGG |
2 |
0.6429 |
Tier III |
| 8 |
NC_000068.7 |
2 |
26446781 |
- |
0610009E02Rik |
NNNGGCATATGGGCACAGAC |
NGG |
2 |
0.0889 |
Tier III |
| 9 |
NC_000067.6 |
1 |
70915311 |
+ |
Gm29906 |
NNNGGCATAATGGCCCAGAC |
NTG |
2 |
0.0334 |
Tier III |
| 10 |
NC_000081.6 |
15 |
67229624 |
- |
1700012I11Rik |
NNNGGCATAATGGCCCAGAC |
NTG |
2 |
0.0334 |
Tier III |
Other clones with same target sequence:
(none)
