Construct: sgRNA BRDN0001146830

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTGGCATATTGGCCCAGAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80231

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 54682640 + KIT NNNAGCATATTGGCCAAGAC NGG 2 0.9 Tier II
2 NC_000023.11 X 13574922 + EGFL6 NNNAGCATATTGGCCCAAAC NGG 2 0.6231 Tier II
3 NC_000016.10 16 90014813 - DBNDD1 NNNGCCATGTTGGCCCAGAC NGG 2 0.5238 Tier II
4 NC_000020.11 20 5066072 + TMEM230 NNNCCCATATTGGCCCAGAC NGG 2 0.416 Tier II
5 NC_000005.10 5 1052880 - SLC12A7 NNNGGCGTATTGGCACAGAC NGG 2 0.1569 Tier II
6 NC_000004.12 4 56668650 - HOPX NNNGGCATAATGGCTCAGAC NGG 2 0.0571 Tier II
7 NC_000013.11 13 23864021 - MIPEP NNNGGCATATTGGACCAGAC NTG 2 0.0136 Tier II
8 NC_000023.11 X 13574922 + RPL30P15 NNNAGCATATTGGCCCAAAC NGG 2 0.6231 Tier III
9 NC_000001.11 1 16160069 + EPHA2-AS1 NNNGGCATTCTGGCCCAGAC NGG 2 0.32 Tier III
10 NC_000002.12 2 47318545 - EPCAM-DT NNNGGCATGTTGGCCCAGGC NGG 2 0.25 Tier III
11 NC_000011.10 11 88346368 - LOC101929174 NNNGGCATATTGGACCAGAG NGG 2 0.0206 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 152736254 + Pigk NNNGGCACATGGGCCCAGAC NGG 2 0.4 Tier II
2 NC_000079.6 13 83534143 + Mef2c NNNGGCTTATTGGTCCAGAC NGG 2 0.3208 Tier II
3 NC_000070.6 4 9156743 - Clvs1 NNNGGCATATTGGTCCAGGC NGG 2 0.275 Tier II
4 NC_000075.6 9 94941584 - Slc9a9 NNNGGCATATTGCCCCAGTC NGG 2 0.2267 Tier II
5 NC_000082.6 16 24509971 + Lpp NNNGGCATGTTGGCTCAGAC NGG 2 0.0444 Tier II
6 NC_000073.6 7 143386693 + Kcnq1 NNNGGCATATTTGCCCAGAC NGC 2 0.0085 Tier II
7 NC_000077.6 11 52663528 + Gm30366 NNNGGTATATTGGCCCAAAC NGG 2 0.6429 Tier III
8 NC_000068.7 2 26446781 - 0610009E02Rik NNNGGCATATGGGCACAGAC NGG 2 0.0889 Tier III
9 NC_000067.6 1 70915311 + Gm29906 NNNGGCATAATGGCCCAGAC NTG 2 0.0334 Tier III
10 NC_000081.6 15 67229624 - 1700012I11Rik NNNGGCATAATGGCCCAGAC NTG 2 0.0334 Tier III
Download CSV

Other clones with same target sequence:

(none)

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