Construct: sgRNA BRDN0001146841
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAACTGTTGTAAAGTGCCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TLK1 (9874)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75997
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
171049892 |
+ |
TLK1 |
NNNCTGTTGTAAAGTGCCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
49048796 |
+ |
IGF2BP1 |
NNNCTGTTGTGAAGTGCTCG |
NGG |
2 |
0.4179 |
Tier II |
| 3 |
NC_000022.11 |
22 |
35919723 |
+ |
RBFOX2 |
NNNCTGTTTTAAAGTGCCCT |
NGG |
2 |
0.3733 |
Tier II |
| 4 |
NC_000016.10 |
16 |
65059694 |
- |
CDH11 |
NNNCAGTTGTCAAGTGCCCG |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000019.10 |
19 |
30323339 |
- |
ZNF536 |
NNNCTGGTGTTAAGTGCCCG |
NGG |
2 |
0.181 |
Tier II |
| 6 |
NC_000009.12 |
9 |
122687010 |
- |
TLK1P1 |
NNNCTGTTGTAAAGTGCCTG |
NGA |
2 |
0.0321 |
Tier III |
| 7 |
NC_000005.10 |
5 |
78666289 |
+ |
LOC124900191 |
NNNCTGTTGTAAAGTGCCTG |
NGT |
2 |
0.0074 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
70745581 |
+ |
Tlk1 |
NNNCTGTGGTAAAGTGCCCA |
NGG |
2 |
0.6875 |
Tier I |
| 2 |
NC_000068.7 |
2 |
28808424 |
- |
Ak8 |
NNNCTGTTGTAAAGTACACG |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000074.6 |
8 |
87814187 |
+ |
Zfp423 |
NNNCTGTTTTAAAGTGCCCA |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000072.6 |
6 |
23457237 |
- |
Cadps2 |
NNNCTCTTGTAAAGTGCCCT |
NGG |
2 |
0.4773 |
Tier II |
| 5 |
NC_000072.6 |
6 |
144306977 |
- |
Sox5 |
NNNCTGTTGGAAAGAGCCCG |
NGG |
2 |
0.2895 |
Tier II |
| 6 |
NC_000074.6 |
8 |
102726870 |
- |
Cdh11 |
NNNCAGTTGTCAAGTGCCCG |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000075.6 |
9 |
48723773 |
- |
Zbtb16 |
NNNCTGTTGTAAAATGCCCG |
NGT |
2 |
0.0121 |
Tier II |
Other clones with same target sequence:
(none)
