Construct: sgRNA BRDN0001146845
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACATAGACGGATCTCAATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK17 (5128)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76788
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
96313326 |
- |
CDK17 |
NNNTAGACGGATCTCAATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
78176260 |
- |
NRXN3 |
NNNTAGAGGGATCTCAAAGG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000020.11 |
20 |
41088716 |
- |
TOP1 |
NNNTAGAAGGATCTCAATGC |
NGG |
2 |
0.2786 |
Tier II |
| 4 |
NC_000013.11 |
13 |
20996113 |
+ |
LATS2 |
NNNTAGACAGATCTCAATGC |
NGG |
2 |
0.2755 |
Tier II |
| 5 |
NC_000022.11 |
22 |
43715892 |
- |
EFCAB6 |
NNNTAGACGGATGTCAATGC |
NGG |
2 |
0.0584 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
93216472 |
+ |
Cdk17 |
NNNTAGACGGATCTCAATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000074.6 |
8 |
3604937 |
- |
Camsap3 |
NNNTAGAGGGATCTCAATGA |
NGG |
2 |
0.6027 |
Tier I |
| 3 |
NC_000073.6 |
7 |
104128115 |
+ |
Ubqln5 |
NNNTAGAAGCATCTCAATGG |
NGG |
2 |
0.26 |
Tier I |
| 4 |
NC_000080.6 |
14 |
35333346 |
+ |
Grid1 |
NNNTAGATGGATCACAATGG |
NGG |
2 |
0.5417 |
Tier II |
| 5 |
NC_000086.7 |
X |
96152894 |
- |
Msn |
NNNTAGACAGAACTCAATGG |
NGG |
2 |
0.5143 |
Tier II |
| 6 |
NC_000074.6 |
8 |
91564887 |
- |
Fto |
NNNTGGAAGGATCTCAATGG |
NGG |
2 |
0.468 |
Tier II |
| 7 |
NC_000080.6 |
14 |
33014605 |
+ |
Lrrc18 |
NNNTGGACAGATCTCAATGG |
NGG |
2 |
0.4629 |
Tier II |
| 8 |
NC_000080.6 |
14 |
33014605 |
+ |
Wdfy4 |
NNNTGGACAGATCTCAATGG |
NGG |
2 |
0.4629 |
Tier II |
| 9 |
NC_000075.6 |
9 |
43368508 |
+ |
Gm36799 |
NNNTAGCTGGATCTCAATGG |
NGG |
2 |
0.3828 |
Tier III |
| 10 |
NC_000072.6 |
6 |
41378764 |
- |
Tcrb |
NNNTAGAGGGCTCTCAATGG |
NGG |
2 |
0.2571 |
Tier III |
Other clones with same target sequence:
(none)
