Construct: sgRNA BRDN0001146851

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTTAGCAAGAACGGAATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK5 (341676)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76117

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 52110386 - NEK5 NNNTAGCAAGAACGGAATGG NGG 0 1.0 Tier I
2 NC_000004.12 4 185339460 + SNX25 NNNTGGCAAGAACGGATTGG NGG 2 0.096 Tier I
3 NC_000005.10 5 171970259 - FBXW11 NNNTACCAAGAACAGAATGG NGG 2 0.5114 Tier II
4 NC_000007.14 7 117155260 + ST7 NNNTAGCAAGAATGGAAAGG NGG 2 0.4667 Tier II
5 NC_000002.12 2 225683021 - NYAP2 NNNTAGGAAGAACAGAATGG NGG 2 0.3529 Tier II
6 NC_000017.11 17 39740689 - GRB7 NNNTAGCCAGAGCGGAATGG NGG 2 0.3095 Tier II
7 NC_000016.10 16 67724387 + RANBP10 NNNTAGCAAGAGAGGAATGG NGG 2 0.2778 Tier II
8 NC_000006.12 6 125793655 - NCOA7 NNNTAGCAAGAAAGGAATAG NGG 2 0.2747 Tier II
9 NC_000010.11 10 77408191 - KCNMA1 NNNGAGCAAGAACGGAATGC NGG 2 0.2679 Tier II
10 NC_000010.11 10 128048703 + PTPRE NNNTAGCAGGAACGGAAGGG NGG 2 0.2222 Tier II
11 NC_000020.11 20 13366982 - TASP1 NNNTAGCAAGAGCTGAATGG NGG 2 0.1926 Tier II
12 NC_000004.12 4 5498190 - STK32B NNNCAGCAAGAACGGAATGG NGA 2 0.0449 Tier II
13 NC_000005.10 5 41002400 + MROH2B NNNTAGCAAGAAGGGAATGG NGC 2 0.003 Tier II
14 NC_000015.10 15 99156660 + TTC23 NNNTCGCAAGAACGGCATGG NGG 2 0.0 Tier II
15 NC_000002.12 2 52306241 - NRXN1-DT NNNTAACAAGAACAGAATGG NGG 2 0.75 Tier III
16 NC_000002.12 2 225683021 - LOC105373914 NNNTAGGAAGAACAGAATGG NGG 2 0.3529 Tier III
17 NC_000003.12 3 70423754 + SAMMSON NNNTAGGAAGAACAGAATGG NGG 2 0.3529 Tier III
18 NC_000005.10 5 114731546 + LOC101927078 NNNTTGCAAGAACAGAATGG NGG 2 0.2727 Tier III
19 NC_000019.10 19 31956163 + LINC01837 NNNTAGCAAGAAAGGAAAGG NGG 2 0.2564 Tier III
20 NC_000004.12 4 185339460 + LOC124900827 NNNTGGCAAGAACGGATTGG NGG 2 0.096 Tier III
21 NC_000009.12 9 107769803 - LOC105376208 NNNTAGCAGGAAGGGAATGG NGG 2 0.0909 Tier III
22 NC_000009.12 9 107769803 - LOC105376209 NNNTAGCAGGAAGGGAATGG NGG 2 0.0909 Tier III
23 NC_000007.14 7 68245300 - LOC105375341 NNNTAACAAGAACGGCATGG NGG 2 0.0 Tier III
24 NC_000007.14 7 83169091 - LOC105375377 NNNTAACAAGAACGGCATGG NGG 2 0.0 Tier III
25 NC_000016.10 16 86277176 + LINC01081 NNNTAACAAGAACGGCATGG NGG 2 0.0 Tier III
26 NC_000001.11 1 229133915 - LINC02815 NNNTAGCAAGAACAGCATGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 22113676 - Nek5 NNNTAGCAAGAATGGAATGG NTG 2 0.0273 Tier I
2 NC_000071.6 5 64178884 - Tbc1d1 NNNTAGCAAGAATGGAAAGG NGG 2 0.4667 Tier II
3 NC_000071.6 5 8832627 - Abcb1b NNNTAGAATGAACGGAATGG NGG 2 0.45 Tier II
4 NC_000085.6 19 29589084 + Ric1 NNNTAGCAAGAACCAAATGG NGG 2 0.4034 Tier II
5 NC_000076.6 10 74525014 + Pcdh15 NNNTAGAAAGAAAGGAATGG NGG 2 0.2885 Tier II
6 NC_000076.6 10 116428508 + Kcnmb4 NNNTAGTAAGAACGGAAGGG NGG 2 0.2708 Tier II
7 NC_000074.6 8 105341046 - Fhod1 NNNAAGGAAGAACGGAATGG NGG 2 0.2241 Tier II
8 NC_000082.6 16 42125843 + Lsamp NNNTAGGAAGAAAGGAATGG NGG 2 0.181 Tier II
9 NC_000079.6 13 13463361 + Nid1 NNNTAGCAACAACTGAATGG NGG 2 0.1067 Tier II
10 NC_000072.6 6 61964483 - Ccser1 NNNTAGGAAGAAGGGAATGG NGG 2 0.0642 Tier II
11 NC_000080.6 14 59744909 - Atp8a2 NNNTAGCAAGAAAGGAATGG NGA 2 0.0267 Tier II
12 NC_000075.6 9 3860580 + Gucy1a2 NNNTAGCAAGAACGCAATGG NGA 2 0.0189 Tier II
13 NC_000076.6 10 44128026 - Crybg1 NNNTAGCAAGAATGGAATGG NGT 2 0.0113 Tier II
14 NC_000077.6 11 96683871 - Skap1 NNNTAGCAAGAACGGCATGC NGG 2 0.0 Tier II
15 NC_000067.6 1 123742402 - Dpp10 NNNTAGCAAGAACGGCATGG NAG 2 0.0 Tier II
16 NC_000082.6 16 12824574 - 1700003L19Rik NNNTAGAAAGGACGGAATGG NGG 2 0.4875 Tier III
17 NC_000082.6 16 12824574 - 4930414F18Rik NNNTAGAAAGGACGGAATGG NGG 2 0.4875 Tier III
18 NC_000085.6 19 33090346 - Gm29863 NNNCAGCAAGAAAGGAATGG NGG 2 0.2489 Tier III
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Other clones with same target sequence:

(none)