Construct: sgRNA BRDN0001146852

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAGCTTGATGTGTCCTGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CIT (11113)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76723

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 119832829 + CIT NNNGCTTGATGTGTCCTGTG NGG 0 1.0 Tier I
2 NC_000019.10 19 39826036 + DYRK1B NNNGCTTGATGTGTCTTGTG NGG 1 0.3077 Tier I
3 NC_000005.10 5 146096981 + PLAC8L1 NNNGGTTGATATGTCCTGTG NGG 2 0.6 Tier II
4 NC_000005.10 5 1436930 - SLC6A3 NNNGCTTGATGCCTCCTGTG NGG 2 0.3989 Tier II
5 NC_000013.11 13 99698094 - CLYBL NNNGTTTCATGTGTCCTGTG NGG 2 0.3916 Tier II
6 NC_000003.12 3 49009629 + WDR6 NNNGCTAGATGTCTCCTGTG NGG 2 0.3684 Tier II
7 NC_000003.12 3 14866837 + FGD5 NNNCCTTCATGTGTCCTGTG NGG 2 0.3258 Tier II
8 NC_000004.12 4 713269 + PCGF3 NNNGCTTCATGGGTCCTGTG NGG 2 0.3077 Tier II
9 NC_000004.12 4 713269 + LOC124900172 NNNGCTTCATGGGTCCTGTG NGG 2 0.3077 Tier II
10 NC_000003.12 3 125026227 + HEG1 NNNGCATGATGTGCCCTGTG NGG 2 0.2476 Tier II
11 NC_000020.11 20 35304574 - UQCC1 NNNTCTTCATGTGTCCTGTG NGG 2 0.2238 Tier II
12 NC_000003.12 3 52539170 - UQCC5 NNNGCTTGCTGTGTCCTGAG NGG 2 0.1633 Tier II
13 NC_000011.10 11 46594004 - AMBRA1 NNNGCTTGATGTCTCCTGAG NGG 2 0.1203 Tier II
14 NC_000001.11 1 97758805 - DPYD NNNGCTTGATGTTTCCTGTG NAG 2 0.0778 Tier II
15 NC_000005.10 5 108148758 - FBXL17 NNNGCCTGATGTGTCCTGTG NGA 2 0.0631 Tier II
16 NC_000004.12 4 124679612 + ANKRD50 NNNACTTGATGTGTGCTGTG NGG 2 0.045 Tier II
17 NC_000014.9 14 73084529 + RBM25 NNNACTTGATGTGTGCTGTG NGG 2 0.045 Tier II
18 NC_000007.14 7 117621472 + CFTR NNNGCTAGATGTGTCCTGTG NGC 2 0.0194 Tier II
19 NC_000010.11 10 51320522 + PRKG1 NNNGCTTGATGTGTCCTGTG NGT 1 0.0161 Tier II
20 NC_000007.14 7 105013135 - KMT2E-AS1 NNNGCCTGATGTGTCCTGTC NGG 2 0.3896 Tier III
21 NC_000014.9 14 97743925 - LOC105370651 NNNGCTTGATGTGTACTTTG NGG 2 0.1481 Tier III
22 NC_000010.11 10 51320522 + RSU1P3 NNNGCTTGATGTGTCCTGTG NGT 1 0.0161 Tier III
23 NC_000017.11 17 36484076 - LOC105371749 NNNGCTTGATTTGTCCTGTG NTG 2 0.015 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 28186603 - Dyrk1b NNNGCTTGATGTGTCTTGTG NGG 1 0.3077 Tier I
2 NC_000071.6 5 115875925 - Cit NNNGCTTGATGTGTCCCGTC NGG 2 0.3025 Tier I
3 NC_000067.6 1 59544572 - Gm973 NNNCCTTGATGTGTCCTGAG NGG 2 0.1513 Tier I
4 NC_000070.6 4 144064686 - Pramel4 NNNACTTGATGAGTCCTGTG NGG 2 0.72 Tier II
5 NC_000070.6 4 144105462 - Gm13102 NNNACTTGATGAGTCCTGTG NGG 2 0.72 Tier II
6 NC_000068.7 2 160979646 + Chd6 NNNGATTAATGTGTCCTGTG NGG 2 0.5714 Tier II
7 NC_000080.6 14 48237514 + Peli2 NNNGCTAGCTGTGTCCTGTG NGG 2 0.5 Tier II
8 NC_000071.6 5 143519092 - Rac1 NNNGCATGCTGTGTCCTGTG NGG 2 0.4952 Tier II
9 NC_000068.7 2 120666648 - Stard9 NNNGCATGATGTGTCCTCTG NGG 2 0.4127 Tier II
10 NC_000080.6 14 70496288 + Bmp1 NNNGCTTGTTGTGACCTGTG NGG 2 0.3714 Tier II
11 NC_000072.6 6 25674036 - Gpr37 NNNGTGTGATGTGTCCTGTG NGG 2 0.3636 Tier II
12 NC_000080.6 14 48237255 + Peli2 NNNGCTGGTTGTGTCCTGTG NGG 2 0.3529 Tier II
13 NC_000080.6 14 48237218 + Peli2 NNNGCTGGCTGTGTCCTGTG NGG 2 0.3361 Tier II
14 NC_000080.6 14 48237292 + Peli2 NNNGCTGGCTGTGTCCTGTG NGG 2 0.3361 Tier II
15 NC_000080.6 14 48237329 + Peli2 NNNGCTGGCTGTGTCCTGTG NGG 2 0.3361 Tier II
16 NC_000080.6 14 48237366 + Peli2 NNNGCTGGCTGTGTCCTGTG NGG 2 0.3361 Tier II
17 NC_000080.6 14 48237403 + Peli2 NNNGCTGGCTGTGTCCTGTG NGG 2 0.3361 Tier II
18 NC_000080.6 14 48237440 + Peli2 NNNGCTGGCTGTGTCCTGTG NGG 2 0.3361 Tier II
19 NC_000080.6 14 48237477 + Peli2 NNNGCTGGCTGTGTCCTGTG NGG 2 0.3361 Tier II
20 NC_000075.6 9 96988292 - Spsb4 NNNCCTTGCTGTGTCCTGTG NGG 2 0.3025 Tier II
21 NC_000074.6 8 84546404 - Cacna1a NNNCCTTGAGGTGTCCTGTG NGG 2 0.2647 Tier II
22 NC_000077.6 11 48846051 - Trim7 NNNTCTTGGTGTGTCCTGTG NGG 2 0.2424 Tier II
23 NC_000080.6 14 79621007 + Sugt1 NNNTCTTGCTGTGTCCTGTG NGG 2 0.2078 Tier II
24 NC_000080.6 14 119736227 + Hs6st3 NNNGCTTGATGTCTCCTCTG NGG 2 0.2005 Tier II
25 NC_000071.6 5 112372584 + Hps4 NNNGGTTGATGTGTCCTGGG NGG 2 0.15 Tier II
26 NC_000072.6 6 5736107 + Dync1i1 NNNGCTTCATGTGTACTGTG NGG 2 0.1368 Tier II
27 NC_000070.6 4 110705329 + Agbl4 NNNGCTTGATGTCTCCTGAG NGG 2 0.1203 Tier II
28 NC_000082.6 16 34931376 + Mylk NNNACTTGATGTGTGCTGTG NGG 2 0.045 Tier II
29 NC_000083.6 17 26606441 - Ergic1 NNNGCTTGCTGTGTGCTGTG NGG 2 0.0286 Tier II
30 NC_000078.6 12 110886273 - Cinp NNNGCTTGAAGTGGCCTGTG NGG 2 0.0 Tier II
31 NC_000067.6 1 105870406 - Gm41926 NNNCCTTGATATGTCCTGTG NGG 2 0.5294 Tier III
32 NC_000067.6 1 59544572 - Gm28411 NNNCCTTGATGTGTCCTGAG NGG 2 0.1513 Tier III
33 NC_000067.6 1 96646016 + 4930533P14Rik NNNGCTTGATGTGTCCTGGG NAG 2 0.0648 Tier III
34 NC_000067.6 1 96646016 + Gm1833 NNNGCTTGATGTGTCCTGGG NAG 2 0.0648 Tier III
35 NC_000080.6 14 24859701 - Gm32008 NNNGCCTGATGTGTTCTGTG NGG 2 0.0606 Tier III
36 NC_000079.6 13 56689465 + Gm33019 NNNGCTTGGTGTGTCCTGTG NGA 2 0.0463 Tier III
37 NC_000081.6 15 58760709 + Gm41329 NNNGCTTGCTGTGTGCTGTG NGG 2 0.0286 Tier III
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Other clones with same target sequence:

(none)