Construct: sgRNA BRDN0001146902

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTAACAACGCAGAAAATAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARK2 (2011)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77592

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000011.10 11 63902669 + MARK2 NNNACAACGCAGAAAATAAG NGG 0 1.0 Tier I
2 NC_000007.14 7 65964374 - GUSB NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier I
3 NC_000022.11 22 50157019 - MOV10L1 NNNACAACCCAGAAAATAAG NGG 1 0.5385 Tier II
4 NC_000019.10 19 9890971 + OLFM2 NNNACAACGAAGAAAAAAAG NGG 2 0.4622 Tier II
5 NC_000006.12 6 75623887 - SENP6 NNNACTACACAGAAAATAAG NGG 2 0.4592 Tier II
6 NC_000016.10 16 17205036 + XYLT1 NNNACAAAACAGAAAATAAG NGG 2 0.4179 Tier II
7 NC_000022.11 22 28888534 - ZNRF3 NNNACAAAACAGAAAATAAG NGG 2 0.4179 Tier II
8 NC_000008.11 8 35369908 - UNC5D NNNGCAACACAGAAAATAAG NGG 2 0.4018 Tier II
9 NC_000002.12 2 73535491 - ALMS1 NNNAAAAGGCAGAAAATAAG NGG 2 0.3673 Tier II
10 NC_000001.11 1 44472377 - RNF220 NNNACAAACCAGAAAATAAG NGG 2 0.35 Tier II
11 NC_000001.11 1 23139661 + LUZP1 NNNACAACACAGAAAATATG NGG 2 0.3462 Tier II
12 NC_000017.11 17 7651143 - ATP1B2 NNNAGAACGCAGAAAAAAAG NGG 2 0.32 Tier II
13 NC_000010.11 10 49630763 + CHAT NNNAGAACTCAGAAAATAAG NGG 2 0.32 Tier II
14 NC_000020.11 20 3403799 - DNAAF9 NNNACAAAGCAGAAAATGAG NGG 2 0.26 Tier II
15 NC_000005.10 5 34175873 - C1QTNF3 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier II
16 NC_000008.11 8 50318140 - SNTG1 NNNACAACTCAGACAATAAG NGG 2 0.1143 Tier II
17 NC_000023.11 X 44333216 + EFHC2 NNNACAACGCAGGAAATAAG NCG 2 0.0699 Tier II
18 NC_000006.12 6 133371370 - EYA4 NNNACAACTCAGAAAAGAAG NGG 2 0.0627 Tier II
19 NC_000010.11 10 82981229 - NRG3 NNNACCACGCAGAAAATAAG NGA 2 0.0316 Tier II
20 NC_000019.10 19 54931968 - NCR1 NNNACCACGCAGAAAATAAG NGA 2 0.0316 Tier II
21 NC_000019.10 19 54931968 - NLRP7 NNNACCACGCAGAAAATAAG NGA 2 0.0316 Tier II
22 NC_000003.12 3 155634200 + PLCH1 NNNACAACTCAGAAAATAAG NTG 2 0.0208 Tier II
23 NC_000023.11 X 76824460 - MIR325HG NNNACAAGGCAGAAAACAAG NGG 2 0.4538 Tier III
24 NC_000002.12 2 217718673 - DIRC3 NNNGCAACGCAGAAAACAAG NGG 2 0.4412 Tier III
25 NC_000022.11 22 49496679 + MIR3667HG NNNACAAAGCACAAAATAAG NGG 2 0.3441 Tier III
26 NC_000006.12 6 57973329 - LOC101927293 NNNACATCACAGAAAATAAG NGG 2 0.2812 Tier III
27 NC_000004.12 4 159890454 - LOC107986324 NNNACAACGAAGTAAATAAG NGG 2 0.26 Tier III
28 NC_000009.12 9 1708198 - LOC105375951 NNNACAACGCAGACAATAAG NGG 1 0.2143 Tier III
29 NC_000023.11 X 146880815 - LOC101928832 NNNACCACGCAGAAAATAGG NGG 2 0.1705 Tier III
30 NC_000005.10 5 21501776 + GUSBP1 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
31 NC_000005.10 5 34175873 - GUSBP18 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
32 NC_000005.10 5 69920817 + GUSBP13 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
33 NC_000005.10 5 70197306 - GUSBP14 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
34 NC_000005.10 5 70795866 + GUSBP16 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
35 NC_000005.10 5 71197697 - GUSBP9 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
36 NC_000006.12 6 26878751 - GUSBP2 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
37 NC_000006.12 6 26878751 - POM121L6P NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
38 NC_000006.12 6 57919835 - GUSBP4 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
39 NC_000007.14 7 64120495 + GUSBP6 NNNAAAACGCAGAAAATACG NGG 2 0.1182 Tier III
40 NC_000004.12 4 20211540 - LOC105374514 NNNACAAAGCAGAAAATAAG NTG 2 0.0253 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 7283141 - Mark2 NNNACAACGCAGAAAATAAG NGG 0 1.0 Tier I
2 NC_000086.7 X 75369002 + F8 NNNACAATGCAGAAAATAAA NGG 2 0.8203 Tier II
3 NC_000071.6 5 38822406 - Clnk NNNACAAAGCAGAAAATAAA NGG 2 0.6094 Tier II
4 NC_000079.6 13 108929955 - Pde4d NNNATAATGCAGAAAATAAG NGG 2 0.5568 Tier II
5 NC_000072.6 6 33679330 - Exoc4 NNNAAAATGCAGAAAATAAG NGG 2 0.5 Tier II
6 NC_000084.6 18 63723087 + Wdr7 NNNACAACGCAGAGAATAAG NGG 1 0.4667 Tier II
7 NC_000069.6 3 30311466 - Mecom NNNACAATGCAGAAAAAAAG NGG 2 0.4667 Tier II
8 NC_000073.6 7 128117163 - Itgam NNNACAGGGCAGAAAATAAG NGG 2 0.4538 Tier II
9 NC_000072.6 6 115218331 - Syn2 NNNAGAACACAGAAAATAAG NGG 2 0.3857 Tier II
10 NC_000080.6 14 60577216 - Spata13 NNNAAAAAGCAGAAAATAAG NGG 2 0.3714 Tier II
11 NC_000083.6 17 32365564 + Wiz NNNACAACACAGAAAAAAAG NGG 2 0.3429 Tier II
12 NC_000077.6 11 59104215 + Obscn NNNACAAGGCAGAAAATTAG NGG 2 0.3214 Tier II
13 NC_000068.7 2 20601724 + Etl4 NNNACAAGGCAGAGAATAAG NGG 2 0.3 Tier II
14 NC_000078.6 12 30072653 - Tpo NNNACAAGGCAGAGAATAAG NGG 2 0.3 Tier II
15 NC_000077.6 11 110136844 + Abca9 NNNACAATGCAGAAAATAAG NAG 2 0.2269 Tier II
16 NC_000075.6 9 62700442 - Itga11 NNNACAAGGCAGAAAATAAG NAG 2 0.1667 Tier II
17 NC_000069.6 3 26281027 + Nlgn1 NNNACAAAGCAGAAAAGAAG NGG 2 0.0765 Tier II
18 NC_000079.6 13 94994743 + Wdr41 NNNACAACGGAGAAAAGAAG NGG 2 0.0458 Tier II
19 NC_000072.6 6 64694185 - Grid2 NNNACAAAGCAGAAAATAAG NGA 2 0.0451 Tier II
20 NC_000074.6 8 56309679 - Hpgd NNNACAACCCAGAAAATAAG NTG 2 0.021 Tier II
21 NC_000080.6 14 55880391 - Cbln3 NNNACAACGCTGAAAATAAG NTG 2 0.012 Tier II
22 NC_000070.6 4 106433819 - Usp24 NNNACAAGGCAGAAAATAAG NGT 2 0.0104 Tier II
23 NC_000072.6 6 143228682 - LOC115490444 NNNACAAAGTAGAAAATAAG NGG 2 0.6118 Tier III
24 NC_000069.6 3 48565879 - Gm46835 NNNACAAGGCAGAAAATAAA NGG 2 0.6027 Tier III
25 NC_000071.6 5 122996373 + A930024E05Rik NNNACAAGGCAGAAAATATG NGG 2 0.3462 Tier III
26 NC_000080.6 14 71715950 - Gm34819 NNNAAAACTCAGAAAATAAG NGG 2 0.3048 Tier III
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Other clones with same target sequence:

(none)