Construct: sgRNA BRDN0001146904
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGCTCACCTGACCACGTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK6 (1021)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75644
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
92833100 |
+ |
CDK6 |
NNNCTCACCTGACCACGTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
134551819 |
+ |
TMEM163 |
NNNCTCACCCAACCACGTTG |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000017.11 |
17 |
151950 |
+ |
DOC2B |
NNNCTCACCTGACCACTCTG |
NGG |
2 |
0.1071 |
Tier II |
| 4 |
NC_000019.10 |
19 |
38946179 |
- |
FBXO17 |
NNNCTCCCCTGACCACCTTG |
NGG |
2 |
0.1029 |
Tier II |
| 5 |
NC_000012.12 |
12 |
49946307 |
- |
LOC105369763 |
NNNCTCACCTGACCAAGTTT |
NGG |
2 |
0.7 |
Tier III |
| 6 |
NC_000019.10 |
19 |
54533969 |
- |
KIR3DX1 |
NNNCTCAGCTGACCACTTTG |
NGG |
2 |
0.1607 |
Tier III |
| 7 |
NC_000009.12 |
9 |
83220224 |
+ |
FRMD3-AS1 |
NNNCTCACCTGACCACCTTC |
NGG |
2 |
0.1008 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
3344591 |
- |
Cdk6 |
NNNCTCACCTGACCACGTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
41265438 |
- |
Gm2832 |
NNNCTCACCTGACCACATTG |
NGG |
1 |
0.9333 |
Tier I |
| 3 |
NC_000073.6 |
7 |
43412511 |
- |
Siglecg |
NNNCTCACATGACCACGATG |
NGG |
2 |
0.5714 |
Tier I |
| 4 |
NC_000073.6 |
7 |
126849763 |
- |
Doc2a |
NNNCTCACCTGAGCACCTTG |
NGG |
2 |
0.0321 |
Tier I |
| 5 |
NC_000076.6 |
10 |
22148498 |
+ |
E030030I06Rik |
NNNCTCACCTGACCACGTAG |
NGT |
2 |
0.0046 |
Tier I |
| 6 |
NC_000072.6 |
6 |
23473072 |
+ |
Cadps2 |
NNNCCCACCTGACCACGTTG |
NGG |
1 |
1.0 |
Tier II |
| 7 |
NC_000080.6 |
14 |
43089776 |
- |
Gm8104 |
NNNCTCACCTGACCACATTG |
NGG |
1 |
0.9333 |
Tier II |
| 8 |
NC_000075.6 |
9 |
101094675 |
- |
Msl2 |
NNNATCACCTGACCAAGTTG |
NGG |
2 |
0.8421 |
Tier II |
| 9 |
NC_000085.6 |
19 |
57921663 |
+ |
Atrnl1 |
NNNCTCACGTGACCACGTTC |
NGG |
2 |
0.2653 |
Tier II |
| 10 |
NC_000068.7 |
2 |
72354287 |
- |
Map3k20 |
NNNCAGACCTGACCACGTTG |
NGG |
2 |
0.25 |
Tier II |
| 11 |
NC_000076.6 |
10 |
75656444 |
- |
Cabin1 |
NNNCTCATCTGACCACTTTG |
NGG |
2 |
0.2188 |
Tier II |
| 12 |
NC_000086.7 |
X |
53974567 |
+ |
Gm15482 |
NNNCTCACCTGACCACGTTG |
NGG |
0 |
1.0 |
Tier III |
| 13 |
NC_000080.6 |
14 |
43169025 |
- |
LOC115488378 |
NNNCTCACCTGACCACATTG |
NGG |
1 |
0.9333 |
Tier III |
| 14 |
NC_000076.6 |
10 |
7390562 |
+ |
Gm21321 |
NNNCTCACTTGACCACGTTG |
NGG |
1 |
0.875 |
Tier III |
| 15 |
NC_000068.7 |
2 |
116807857 |
+ |
Gm13990 |
NNNCTCACCTGACCACAATG |
NGG |
2 |
0.6222 |
Tier III |
| 16 |
NC_000085.6 |
19 |
57921663 |
+ |
Gm52385 |
NNNCTCACGTGACCACGTTC |
NGG |
2 |
0.2653 |
Tier III |
| 17 |
NC_000080.6 |
14 |
43797507 |
- |
LOC115488355 |
NNNCTCAACTGACCATGTTG |
NGG |
2 |
0.2 |
Tier III |
| 18 |
NC_000079.6 |
13 |
3361379 |
- |
Gm16505 |
NNNCTCACCTGACCACGTTG |
NGT |
1 |
0.0161 |
Tier III |
Other clones with same target sequence:
(none)
