Construct: sgRNA BRDN0001146937
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCGGAGTAGGGTGTTTCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MKNK1 (8569)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75513
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
46576612 |
- |
MKNK1 |
NNNGAGTAGGGTGTTTCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
39395929 |
- |
KIF21A |
NNNGAGTGGGGTGTTTAGAG |
NGG |
2 |
0.3422 |
Tier II |
| 3 |
NC_000023.11 |
X |
153706515 |
- |
BCAP31 |
NNNGAGGAGGGTGTTGCGAG |
NGG |
2 |
0.2036 |
Tier II |
| 4 |
NC_000020.11 |
20 |
349526 |
+ |
NRSN2 |
NNNGTGTAGGGTGTCTCGAG |
NGG |
2 |
0.0992 |
Tier II |
| 5 |
NC_000002.12 |
2 |
17584692 |
- |
VSNL1 |
NNNGAGTAGGGTGTGACGAG |
NGG |
2 |
0.0455 |
Tier II |
| 6 |
NC_000012.12 |
12 |
115164827 |
- |
LOC124903081 |
NNNGAGTAGGGTGTTTTGCG |
NGG |
2 |
0.0966 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
166538548 |
- |
Egfl6 |
NNNGAGGAGGGTGTTTCCAG |
NGG |
2 |
0.2801 |
Tier I |
| 2 |
NC_000073.6 |
7 |
133078082 |
- |
Ctbp2 |
NNNGAGAAGGGTATTTCGAG |
NGG |
2 |
0.8077 |
Tier II |
| 3 |
NC_000067.6 |
1 |
139639238 |
+ |
Cfhr3 |
NNNGAGTAGGGAGTTTCTAG |
NGG |
2 |
0.5333 |
Tier II |
| 4 |
NC_000069.6 |
3 |
28661326 |
+ |
Tnik |
NNNGAGTAGGGAGTTTCCAG |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000086.7 |
X |
83194674 |
- |
Dmd |
NNNGGGTAGGGTGTTTCCAG |
NGG |
2 |
0.3429 |
Tier II |
| 6 |
NC_000070.6 |
4 |
9248513 |
+ |
Clvs1 |
NNNGAGTAGGGGGTTTCCAG |
NGG |
2 |
0.2381 |
Tier II |
| 7 |
NC_000071.6 |
5 |
114608646 |
- |
Fam222a |
NNNGAGAAGGGTGTTTGGAG |
NGG |
2 |
0.0515 |
Tier II |
| 8 |
NC_000068.7 |
2 |
27893542 |
+ |
Col5a1 |
NNNGAGTAGGGTGTGTAGAG |
NGG |
2 |
0.0233 |
Tier II |
| 9 |
NC_000067.6 |
1 |
15875571 |
+ |
Sbspon |
NNNGAGTAGGGTGTTTGGGG |
NGG |
2 |
0.0221 |
Tier II |
| 10 |
NC_000071.6 |
5 |
44461338 |
+ |
Ldb2 |
NNNGAGTAGGGTGTTTGGAG |
NTG |
2 |
0.0023 |
Tier II |
| 11 |
NC_000084.6 |
18 |
13550301 |
- |
Gm41670 |
NNNCAGTAGGGTGTTTCCAG |
NGG |
2 |
0.2521 |
Tier III |
Other clones with same target sequence:
(none)
