Construct: sgRNA BRDN0001146959
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACGCTACCTTCCAAAACACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSF1R (1436)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77036
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150080840 |
- |
CSF1R |
NNNCTACCTTCCAAAACACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
42109339 |
- |
GLI3 |
NNNCTACTTTCCAAAACATG |
NGG |
2 |
0.4038 |
Tier II |
| 3 |
NC_000005.10 |
5 |
177010459 |
- |
UIMC1 |
NNNCTACCTTCAAAAACATG |
NGG |
2 |
0.3297 |
Tier II |
| 4 |
NC_000018.10 |
18 |
77097776 |
- |
MBP |
NNNCTGCCTTCCAAAACACC |
NGG |
2 |
0.3061 |
Tier II |
| 5 |
NC_000005.10 |
5 |
110567136 |
+ |
TMEM232 |
NNNGTACCTTCCATAACACG |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000010.11 |
10 |
54627349 |
- |
PCDH15 |
NNNCTCCCTCCCAAAACACG |
NGG |
2 |
0.2424 |
Tier II |
| 7 |
NC_000005.10 |
5 |
84314625 |
+ |
EDIL3 |
NNNCTTCCTTCCAAAACACG |
NTG |
2 |
0.0278 |
Tier II |
| 8 |
NC_000017.11 |
17 |
73817957 |
- |
LINC00469 |
NNNCTGCCTTCCAAAACATG |
NGG |
2 |
0.3297 |
Tier III |
| 9 |
NC_000010.11 |
10 |
54627349 |
- |
LOC105378311 |
NNNCTCCCTCCCAAAACACG |
NGG |
2 |
0.2424 |
Tier III |
| 10 |
NC_000008.11 |
8 |
41182368 |
+ |
LOC105379771 |
NNNCTACCTTCCAAAGCACT |
NGG |
2 |
0.1346 |
Tier III |
| 11 |
NC_000014.9 |
14 |
44102000 |
+ |
LINC02307 |
NNNCTACCTCCCACAACACG |
NGG |
2 |
0.1143 |
Tier III |
| 12 |
NC_000002.12 |
2 |
198631904 |
+ |
LOC105373831 |
NNNCTACCTTCCAAAACAGG |
NGA |
2 |
0.0087 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
4086994 |
- |
Mthfd1l |
NNNCTACACTCCAAAACACG |
NGG |
2 |
0.6 |
Tier II |
| 2 |
NC_000085.6 |
19 |
16139893 |
- |
Gnaq |
NNNCTACCTCCTAAAACACG |
NGG |
2 |
0.2872 |
Tier II |
| 3 |
NC_000072.6 |
6 |
32847164 |
- |
Chchd3 |
NNNCTACCTTCCATAAAACG |
NGG |
2 |
0.2489 |
Tier II |
| 4 |
NC_000068.7 |
2 |
178453641 |
+ |
Cdh26 |
NNNCTACCTTCCAAAGCATG |
NGG |
2 |
0.0888 |
Tier II |
| 5 |
NC_000068.7 |
2 |
70573023 |
- |
Gad1 |
NNNCTACCTTCCAAAACAGC |
NGG |
2 |
0.0536 |
Tier II |
| 6 |
NC_000077.6 |
11 |
110299169 |
+ |
Abca5 |
NNNCTACCTTCCCAAACACG |
NGT |
2 |
0.0034 |
Tier II |
| 7 |
NC_000076.6 |
10 |
4086994 |
- |
Gm40591 |
NNNCTACACTCCAAAACACG |
NGG |
2 |
0.6 |
Tier III |
Other clones with same target sequence:
(none)
