Construct: sgRNA BRDN0001146980

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TAGTGTCGAGCATGGAACCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMG1 (23049)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75770

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000016.10 16 18841700 + SMG1 NNNTGTCGAGCATGGAACCG NGG 0 1.0 Tier I
2 NC_000017.11 17 79407985 + RBFOX3 NNNTGTCGAGCACGGAACCC NGG 2 0.3383 Tier II
3 NC_000011.10 11 27597129 + BDNF-AS NNNTGTCAAGCATGGAGCCG NGG 2 0.1765 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 144637671 + Ano1 NNNTGTTGAGCATGGAACCT NGG 2 0.5688 Tier II
2 NC_000067.6 1 180446086 - Stum NNNTGTCGAGGATGGAACCC NGG 2 0.1071 Tier II
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Other clones with same target sequence:

(none)