Construct: sgRNA BRDN0001146986

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCTGCAGGTGTATACAGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPIP5K1 (9677)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76307

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 43581380 - PPIP5K1 NNNTGCAGGTGTATACAGTG NGG 0 1.0 Tier I
2 NC_000008.11 8 120772688 - SNTB1 NNNGGCAGGTATATACAGTG NGG 2 0.625 Tier II
3 NC_000008.11 8 15582353 + TUSC3 NNNTGAAGGTGTAAACAGTG NGG 2 0.5748 Tier II
4 NC_000010.11 10 1547345 + ADARB2 NNNTGCAGGTGTATACACTG NGG 1 0.4762 Tier II
5 NC_000007.14 7 154163835 + DPP6 NNNTGCAGGTGTGTGCAGTG NGG 2 0.4239 Tier II
6 NC_000011.10 11 13451298 - BTBD10 NNNTGGAGGTGTATACAGTT NGG 2 0.35 Tier II
7 NC_000010.11 10 1547196 + ADARB2 NNNCGCAGGTGTATACACTG NGG 2 0.3081 Tier II
8 NC_000022.11 22 17460158 - CECR2 NNNTGCAAGTGTATACAGAG NGG 2 0.2857 Tier II
9 NC_000004.12 4 171929977 - GALNTL6 NNNTGCAGGTGCATTCAGTG NGG 2 0.1895 Tier II
10 NC_000002.12 2 11596771 + GREB1 NNNCGCAGGTGTACACAGTG NGG 2 0.1849 Tier II
11 NC_000014.9 14 59356914 - DAAM1 NNNTGCAGGTGTATAAGGTG NGG 2 0.1765 Tier II
12 NC_000017.11 17 9305018 + STX8 NNNTTCAGCTGTATACAGTG NGG 2 0.1615 Tier II
13 NC_000023.11 X 2475262 - DHRSX NNNTTCAGTTGTATACAGTG NGG 2 0.16 Tier II
14 NC_000024.10 Y 2475262 - DHRSX NNNTTCAGTTGTATACAGTG NGG 2 0.16 Tier II
15 NC_000011.10 11 61344034 + TKFC NNNTGCAGGAGTATACGGTG NGG 2 0.1513 Tier II
16 NC_000022.11 22 50719077 - SHANK3 NNNTGCAGGTGTGTTCAGTG NGG 2 0.1304 Tier II
17 NC_000009.12 9 132020409 - MED27 NNNTTCAGGTGTACACAGTG NGG 2 0.0857 Tier II
18 NC_000005.10 5 177065610 + ZNF346 NNNTGCAGGTTTCTACAGTG NGG 2 0.081 Tier II
19 NC_000002.12 2 11596651 + GREB1 NNNTGCAGGTGTATACAGTG NGA 1 0.0694 Tier II
20 NC_000007.14 7 111560590 - IMMP2L NNNTGCAGGTCTATACAGTG NTG 2 0.0167 Tier II
21 NC_000015.10 15 43681215 - PPIP5K1P1 NNNTGCAGGTGTATACAGTG NGG 0 1.0 Tier III
22 NC_000015.10 15 43681215 - PPIP5K1P1-CATSPER2 NNNTGCAGGTGTATACAGTG NGG 0 1.0 Tier III
23 NC_000008.11 8 120772688 - LOC101927543 NNNGGCAGGTATATACAGTG NGG 2 0.625 Tier III
24 NC_000006.12 6 8297570 - LOC105374911 NNNTGCAGGTGAATACAGTT NGG 2 0.56 Tier III
25 NC_000005.10 5 38782429 + OSMR-DT NNNTGCAGAAGTATACAGTG NGG 2 0.551 Tier III
26 NC_000010.11 10 1547345 + ADARB2-AS1 NNNTGCAGGTGTATACACTG NGG 1 0.4762 Tier III
27 NC_000005.10 5 91310237 - LUCAT1 NNNTGCAGGTGTGTACACTG NGG 2 0.3106 Tier III
28 NC_000010.11 10 1547196 + ADARB2-AS1 NNNCGCAGGTGTATACACTG NGG 2 0.3081 Tier III
29 NC_000011.10 11 111332986 + LOC124902755 NNNTGCAGGTGTAAACACTG NGG 2 0.2948 Tier III
30 NC_000002.12 2 176754949 - LOC101929963 NNNTGCAGGGGTATACAGAG NGG 2 0.1429 Tier III
31 NC_000002.12 2 114004717 + LINC01191 NNNTGCAGGTCTATACAGTG NAG 2 0.1111 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 155861931 - Tas1r3 NNNTGCAGCTGTGTACAGTG NGG 2 0.3512 Tier I
2 NC_000068.7 2 121347634 - Ppip5k1 NNNTGCAGGTGTACACAGTG NGG 1 0.2857 Tier I
3 NC_000071.6 5 125746952 - Tmem132b NNNTACAGGTGTATACAGTA NGG 2 0.8125 Tier II
4 NC_000072.6 6 39582399 + Adck2 NNNTCCAGGTGCATACAGTG NGG 2 0.7444 Tier II
5 NC_000070.6 4 106633266 - Ttc22 NNNTCCAGGTGAATACAGTG NGG 2 0.6286 Tier II
6 NC_000068.7 2 41004970 + Lrp1b NNNTGAATGTGTATACAGTG NGG 2 0.5804 Tier II
7 NC_000077.6 11 87593739 + Mtmr4 NNNTGCAGGTGAATGCAGTG NGG 2 0.52 Tier II
8 NC_000072.6 6 73160578 - Dnah6 NNNTCCAGATGTATACAGTG NGG 2 0.5051 Tier II
9 NC_000079.6 13 9727242 - Zmynd11 NNNTGCTGGTGAATACAGTG NGG 2 0.35 Tier II
10 NC_000072.6 6 147571530 + Ccdc91 NNNTTCAGGTATATACAGTG NGG 2 0.3 Tier II
11 NC_000072.6 6 18455998 + Cttnbp2 NNNTACAGGTGTATATAGTG NGG 2 0.2667 Tier II
12 NC_000078.6 12 101838638 - Trip11 NNNTGCAGGAGTATCCAGTG NGG 2 0.2338 Tier II
13 NC_000072.6 6 73187611 - Dnah6 NNNTGCAGGTGAACACAGTG NGG 2 0.2286 Tier II
14 NC_000068.7 2 165446931 - Slc13a3 NNNTGCAGGTGTTTACATTG NGG 2 0.2 Tier II
15 NC_000067.6 1 43451554 + Nck2 NNNTGCAGGTGTATAGAGTA NGG 2 0.1442 Tier II
16 NC_000079.6 13 62532087 - Zfp934 NNNTGAAGGTGTATACTGTG NGG 2 0.1238 Tier II
17 NC_000067.6 1 82869094 - Agfg1 NNNTGCAGGTGTATACCATG NGG 2 0.1222 Tier II
18 NC_000085.6 19 16291462 - Gnaq NNNTGCAGGTGTATATAGTG NAG 2 0.0798 Tier II
19 NC_000079.6 13 55446999 - Grk6 NNNTGCAGGTGTGTACAGTG NTG 2 0.0254 Tier II
20 NC_000076.6 10 20068937 + Map3k5 NNNTGCAGGCGTATACAGTG NTG 2 0.0208 Tier II
21 NC_000084.6 18 25678645 - Celf4 NNNTGCAGGCGTATACAGTG NGC 2 0.0119 Tier II
22 NC_000085.6 19 20894831 - Tmc1 NNNTGCAGGTGTATACACTG NGC 2 0.0106 Tier II
23 NC_000071.6 5 108682263 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
24 NC_000071.6 5 108682310 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
25 NC_000071.6 5 108682357 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
26 NC_000071.6 5 108682403 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
27 NC_000071.6 5 108682450 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
28 NC_000071.6 5 108682497 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
29 NC_000071.6 5 108682544 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
30 NC_000071.6 5 108682591 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
31 NC_000071.6 5 108682638 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
32 NC_000071.6 5 108682685 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
33 NC_000071.6 5 108682732 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
34 NC_000071.6 5 108682779 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
35 NC_000071.6 5 108682826 - Idua NNNTGCAGGTGTGTACAGTG NGT 2 0.0105 Tier II
36 NC_000067.6 1 126434415 + Nckap5 NNNTGCAGGTGTATAGAGTG NGT 2 0.0025 Tier II
37 NC_000073.6 7 24306640 - Zfp94 NNNTGCAGGTGCAGACAGTG NGG 2 0.0 Tier II
38 NC_000080.6 14 48690503 - Otx2os1 NNNTGCAGGTGCATGCAGTG NGG 2 0.6158 Tier III
39 NC_000068.7 2 165446931 - Gm14437 NNNTGCAGGTGTTTACATTG NGG 2 0.2 Tier III
40 NC_000067.6 1 186484157 - Gm35426 NNNTGCAGCTGTATACAGTG NGT 2 0.0087 Tier III
41 NC_000073.6 7 24306640 - Gm51470 NNNTGCAGGTGCAGACAGTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)