Construct: sgRNA BRDN0001146987

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAAAACAAGTATATCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STYK1 (55359)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75697

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 10627669 - STYK1 NNNAAACAAGTATATCACAT NGG 0 1.0 Tier I
2 NC_000023.11 X 150663813 - MTM1 NNNAAACAATTATATCACAT NGG 1 0.8125 Tier II
3 NC_000013.11 13 78627892 - OBI1 NNNAAACAAGTAAATAACAT NGG 2 0.6923 Tier II
4 NC_000007.14 7 133832889 + EXOC4 NNNAAACAAGTATATCACAA NGG 1 0.5625 Tier II
5 NC_000003.12 3 178669158 + KCNMB2 NNNAAAAAAGTAAATCACAT NGG 2 0.5192 Tier II
6 NC_000002.12 2 60770431 - PAPOLG NNNAAACAATTATAACACAT NGG 2 0.4704 Tier II
7 NC_000008.11 8 91211306 + SLC26A7 NNNAAAAAAGTATATCACAA NGG 2 0.4219 Tier II
8 NC_000008.11 8 91211306 + LRRC69 NNNAAAAAAGTATATCACAA NGG 2 0.4219 Tier II
9 NC_000002.12 2 72506765 - EXOC6B NNNAACCAATTATATCACAT NGG 2 0.3693 Tier II
10 NC_000014.9 14 92729573 + LGMN NNNTAACAAGTATAACACAT NGG 2 0.3684 Tier II
11 NC_000003.12 3 188857119 + LPP NNNAAAATAGTATATCACAT NGG 2 0.3214 Tier II
12 NC_000007.14 7 140576228 - DENND2A NNNATACAAGTAAATCACAT NGG 2 0.2517 Tier II
13 NC_000016.10 16 22020858 - MOSMO NNNGAACAACTATATCACAT NGG 2 0.25 Tier II
14 NC_000010.11 10 63406076 + JMJD1C NNNAAACAAGAATATTACAT NGG 2 0.2308 Tier II
15 NC_000004.12 4 53382851 - FIP1L1 NNNAAATAAGTAGATCACAT NGG 2 0.212 Tier II
16 NC_000005.10 5 52953134 + ITGA1 NNNAAACAAGTATATTAAAT NGG 2 0.1657 Tier II
17 NC_000001.11 1 171317792 - FMO4 NNNAAACAAGGATATTACAT NGG 2 0.1231 Tier II
18 NC_000010.11 10 73048086 + P4HA1 NNNAAACAAGTATGTCACAT NAG 2 0.121 Tier II
19 NC_000010.11 10 106946165 + SORCS1 NNNAAACAAGTGTATGACAT NGG 2 0.1111 Tier II
20 NC_000004.12 4 86335348 + MAPK10 NNNAAACAAGTATAAGACAT NGG 2 0.0891 Tier II
21 NC_000001.11 1 58049534 + DAB1 NNNAACCAAGTATATGACAT NGG 2 0.0699 Tier II
22 NC_000007.14 7 43781346 - BLVRA NNNAAAAAAGTATATCACAC NGG 2 0.0682 Tier II
23 NC_000001.11 1 159187362 + CADM3 NNNAAACAAGTAAATCACAC NGG 2 0.0629 Tier II
24 NC_000011.10 11 9183281 - DENND5A NNNAAACAAGTCTATGACAT NGG 2 0.0405 Tier II
25 NC_000023.11 X 53668642 + HUWE1 NNNAAACAAGTCTATCTCAT NGG 2 0.0351 Tier II
26 NC_000018.10 18 9285459 + ANKRD12 NNNAAAAAAGTATATCACAT NTG 2 0.0292 Tier II
27 NC_000003.12 3 12488627 - TSEN2 NNNAAACAAGTATATAACAT NGC 2 0.0222 Tier II
28 NC_000002.12 2 233430504 - DGKD NNNAAACATGTATATCACAT NGT 2 0.0097 Tier II
29 NC_000004.12 4 80335357 - CFAP299 NNNAAACAAGTATATCAAAT NGT 2 0.0087 Tier II
30 NC_000005.10 5 125238806 - LOC101927421 NNNAAACAATCATATCACAT NGG 2 0.5417 Tier III
31 NC_000003.12 3 34879055 - LOC101928135 NNNAAACAAATATAACACAT NGG 2 0.5404 Tier III
32 NC_000009.12 9 21734128 - LOC107987026 NNNAAGAAAGTATATCACAT NGG 2 0.5357 Tier III
33 NC_000003.12 3 178669158 + KCNMB2-AS1 NNNAAAAAAGTAAATCACAT NGG 2 0.5192 Tier III
34 NC_000010.11 10 23562049 + LOC105376454 NNNAAACAAGTATATCAATT NGG 2 0.2899 Tier III
35 NC_000004.12 4 157814773 + LOC105377509 NNNAAACTAGTATATCAAAT NGG 2 0.2308 Tier III
36 NC_000005.10 5 52953134 + ITGA2-AS1 NNNAAACAAGTATATTAAAT NGG 2 0.1657 Tier III
37 NC_000022.11 22 23655823 - GUSBP11 NNNAAACAAGGCTATCACAT NGG 2 0.1053 Tier III
38 NC_000010.11 10 57390172 - LOC105378313 NNNAAATAAGTATAGCACAT NGG 2 0.0406 Tier III
39 NC_000002.12 2 8033351 + LINC00299 NNNAAACATGTATATCACAT NGC 2 0.0133 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 131307099 - Styk1 NNNAAACAAATATATCACAT NGG 1 0.9333 Tier I
2 NC_000081.6 15 76596425 - Cpsf1 NNNAAACAAGCATATCACAT NGT 2 0.0108 Tier I
3 NC_000067.6 1 159890210 + Tnr NNNAAGCAAGTATATCACAT NGG 1 0.7143 Tier II
4 NC_000067.6 1 32616332 - Khdrbs2 NNNAAAAAAGTATATCACTT NGG 2 0.4038 Tier II
5 NC_000068.7 2 90763913 - Fnbp4 NNNAGACAAGTATTTCACAT NGG 2 0.384 Tier II
6 NC_000068.7 2 59636120 + Tanc1 NNNACACATGTATATCACAT NGG 2 0.3 Tier II
7 NC_000071.6 5 67957308 - Gm5108 NNNAAACCAGTAAATCACAT NGG 2 0.2967 Tier II
8 NC_000069.6 3 139484901 + Stpg2 NNNAAACAAGTATATTATAT NGG 2 0.1978 Tier II
9 NC_000076.6 10 64000035 + Lrrtm3 NNNGAACAAGTCTATCACAT NGG 2 0.1645 Tier II
10 NC_000076.6 10 64000035 + Ctnna3 NNNGAACAAGTCTATCACAT NGG 2 0.1645 Tier II
11 NC_000084.6 18 5051058 - Svil NNNAAACAAGTGTATCACAG NGG 2 0.1275 Tier II
12 NC_000080.6 14 39297433 - Nrg3 NNNAAACAAGCATATCACAG NGG 2 0.1176 Tier II
13 NC_000073.6 7 57295038 + Gabrg3 NNNAAAGAAGTATATCTCAT NGG 2 0.0627 Tier II
14 NC_000071.6 5 150799719 + Pds5b NNNAAACATGTATATCACAT NGT 2 0.0097 Tier II
15 NC_000069.6 3 97736871 + Pde4dip NNNAAACAAGTATATCACTT NGT 2 0.0087 Tier II
16 NC_000078.6 12 70855330 - Frmd6 NNNAAACAAGTATGTCACAT NGT 2 0.0075 Tier II
17 NC_000073.6 7 57295038 + Gm39017 NNNAAAGAAGTATATCTCAT NGG 2 0.0627 Tier III
18 NC_000072.6 6 6678973 + Gm20618 NNNAAACAACTATATCACAC NGG 2 0.0364 Tier III
19 NC_000069.6 3 97736871 + Gm31305 NNNAAACAAGTATATCACTT NGT 2 0.0087 Tier III
Download CSV

Other clones with same target sequence:

(none)