Construct: sgRNA BRDN0001146995
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCACCAGCTCACAGTAATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SCYL1 (57410)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76065
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
65526986 |
- |
SCYL1 |
NNNACCAGCTCACAGTAATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
54775868 |
+ |
RP1 |
NNNACCAGCACACAGTAATG |
NGG |
1 |
0.8571 |
Tier II |
| 3 |
NC_000007.14 |
7 |
2579425 |
+ |
IQCE |
NNNACCAGATCACTGTAATG |
NGG |
2 |
0.4571 |
Tier II |
| 4 |
NC_000007.14 |
7 |
74681568 |
+ |
GTF2I |
NNNACCAGCCCACAGTAATT |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000016.10 |
16 |
88172059 |
+ |
ZNF469 |
NNNGCCAGCTCACTGTAATG |
NGG |
2 |
0.3333 |
Tier II |
| 6 |
NC_000007.14 |
7 |
112157199 |
+ |
DOCK4 |
NNNAGCAGCTCACAGTATTG |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000009.12 |
9 |
439884 |
+ |
DOCK8 |
NNNACCAGCTTACAGAAATG |
NGG |
2 |
0.2797 |
Tier II |
| 8 |
NC_000003.12 |
3 |
112174930 |
- |
SLC9C1 |
NNNACCAGCTCACAGAAAAG |
NGG |
2 |
0.2597 |
Tier II |
| 9 |
NC_000021.9 |
21 |
7785052 |
+ |
LOC105379504 |
NNNACCAGCTCCCAGAAATG |
NGG |
2 |
0.2392 |
Tier II |
| 10 |
NC_000021.9 |
21 |
7785052 |
+ |
LOC107983987 |
NNNACCAGCTCCCAGAAATG |
NGG |
2 |
0.2392 |
Tier II |
| 11 |
NC_000021.9 |
21 |
34415064 |
+ |
SMIM34 |
NNNACCAGCTCCCAGAAATG |
NGG |
2 |
0.2392 |
Tier II |
| 12 |
NC_000015.10 |
15 |
67095075 |
- |
SMAD3 |
NNNACCAGCTCACAGCAAGG |
NGG |
2 |
0.1667 |
Tier II |
| 13 |
NC_000010.11 |
10 |
125808244 |
+ |
UROS |
NNNACCTGCTCACAGGAATG |
NGG |
2 |
0.1514 |
Tier II |
| 14 |
NC_000014.9 |
14 |
91837677 |
+ |
TC2N |
NNNACCAGATCACATTAATG |
NGG |
2 |
0.1224 |
Tier II |
| 15 |
NC_000021.9 |
21 |
15039489 |
- |
NRIP1 |
NNNACCATCTCACATTAATG |
NGG |
2 |
0.0893 |
Tier II |
| 16 |
NC_000015.10 |
15 |
76445507 |
- |
SCAPER |
NNNCCCAGCTGACAGTAATG |
NGG |
2 |
0.0882 |
Tier II |
| 17 |
NC_000008.11 |
8 |
3314907 |
+ |
CSMD1 |
NNNACGAGCTCACAGTAATG |
NGA |
2 |
0.0347 |
Tier II |
| 18 |
NC_000020.11 |
20 |
38157829 |
+ |
TGM2 |
NNNACCAGCTCACAGTAATC |
NTG |
2 |
0.0167 |
Tier II |
| 19 |
NC_000018.10 |
18 |
34513173 |
- |
DTNA |
NNNTCCAGCTCACAGTAATG |
NGC |
2 |
0.0141 |
Tier II |
| 20 |
NC_000010.11 |
10 |
84958033 |
- |
LOC107984249 |
NNNATCAGCTCACAGTAATC |
NGG |
2 |
0.2727 |
Tier III |
| 21 |
NC_000021.9 |
21 |
34415064 |
+ |
LOC105372793 |
NNNACCAGCTCCCAGAAATG |
NGG |
2 |
0.2392 |
Tier III |
| 22 |
NC_000016.10 |
16 |
54868100 |
- |
LOC105371275 |
NNNACCAGCTCACACTATTG |
NGG |
2 |
0.1364 |
Tier III |
| 23 |
NC_000014.9 |
14 |
38964823 |
+ |
LOC102723516 |
NNNACCAGCCCACAGTACTG |
NGG |
2 |
0.1016 |
Tier III |
| 24 |
NC_000022.11 |
22 |
49550949 |
+ |
MIR3667HG |
NNNAACAGCTCACAGTTATG |
NGG |
2 |
0.0762 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
52260543 |
+ |
Tcf7 |
NNNACCAGCTCACAGTATGG |
NGG |
2 |
0.125 |
Tier I |
| 2 |
NC_000085.6 |
19 |
5769601 |
+ |
Scyl1 |
NNNACCAGTTCACAGTAATG |
NGT |
2 |
0.0141 |
Tier I |
| 3 |
NC_000068.7 |
2 |
44794609 |
+ |
Gtdc1 |
NNNACCAGCTCACAGTAATG |
NGG |
0 |
1.0 |
Tier II |
| 4 |
NC_000074.6 |
8 |
88699934 |
- |
Cyld |
NNNACAAGTTCACAGTAATG |
NGG |
2 |
0.8125 |
Tier II |
| 5 |
NC_000068.7 |
2 |
124448892 |
+ |
Sema6d |
NNNACCAGTACACAGTAATG |
NGG |
2 |
0.75 |
Tier II |
| 6 |
NC_000068.7 |
2 |
103297232 |
+ |
Ehf |
NNNACCAGCTAATAGTAATG |
NGG |
2 |
0.525 |
Tier II |
| 7 |
NC_000070.6 |
4 |
5652589 |
- |
Fam110b |
NNNAGCAGGTCACAGTAATG |
NGG |
2 |
0.3714 |
Tier II |
| 8 |
NC_000073.6 |
7 |
110857934 |
+ |
Lyve1 |
NNNACCAGCTCACAGGAATG |
NGG |
1 |
0.3462 |
Tier II |
| 9 |
NC_000074.6 |
8 |
122897016 |
- |
Ankrd11 |
NNNACCAGCTTACAGAAATG |
NGG |
2 |
0.2797 |
Tier II |
| 10 |
NC_000077.6 |
11 |
75254968 |
- |
Rtn4rl1 |
NNNACCTGCTCCCAGTAATG |
NGG |
2 |
0.1151 |
Tier II |
| 11 |
NC_000069.6 |
3 |
154158450 |
- |
Slc44a5 |
NNNAACAGCTCACAGTGATG |
NGG |
2 |
0.1008 |
Tier II |
| 12 |
NC_000080.6 |
14 |
37043373 |
- |
Rgr |
NNNACCAGCTCCCAGTAATG |
NAG |
2 |
0.0682 |
Tier II |
| 13 |
NC_000080.6 |
14 |
37043373 |
- |
Lrit1 |
NNNACCAGCTCCCAGTAATG |
NAG |
2 |
0.0682 |
Tier II |
| 14 |
NC_000080.6 |
14 |
34907469 |
- |
Grid1 |
NNNACCAGCTCACAGTACAG |
NGG |
2 |
0.0544 |
Tier II |
| 15 |
NC_000073.6 |
7 |
82646753 |
+ |
Saxo2 |
NNNACCAGCTCACAGAAATG |
NTG |
2 |
0.0354 |
Tier II |
| 16 |
NC_000074.6 |
8 |
94353270 |
+ |
Slc12a3 |
NNNTCCAGCTCACAGTAATG |
NTG |
2 |
0.0248 |
Tier II |
| 17 |
NC_000070.6 |
4 |
139277141 |
+ |
Capzb |
NNNACCAGCTCACAGAAATG |
NGC |
2 |
0.0202 |
Tier II |
| 18 |
NC_000074.6 |
8 |
122897016 |
- |
Gm39273 |
NNNACCAGCTTACAGAAATG |
NGG |
2 |
0.2797 |
Tier III |
| 19 |
NC_000073.6 |
7 |
36043819 |
+ |
Gm36286 |
NNNACCACCTCCCAGTAATG |
NGG |
2 |
0.1619 |
Tier III |
| 20 |
NC_000077.6 |
11 |
75254968 |
- |
Gm35117 |
NNNACCTGCTCCCAGTAATG |
NGG |
2 |
0.1151 |
Tier III |
| 21 |
NC_000071.6 |
5 |
28909147 |
+ |
Gm35688 |
NNNACCAGCTCACAGTAAGG |
NGA |
2 |
0.0174 |
Tier III |
Other clones with same target sequence:
(none)
