Construct: sgRNA BRDN0001147002

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTGTCACAAAGATGTCTACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK14 (5218)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76638

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 90726588 + CDK14 NNNTCACAAAGATGTCTACA NGG 0 1.0 Tier I
2 NC_000004.12 4 21217349 + KCNIP4 NNNTCTCAAAGAAGTCTACA NGG 2 0.4945 Tier II
3 NC_000010.11 10 72380232 + MICU1 NNNTCTGAAAGATGTCTACA NGG 2 0.3361 Tier II
4 NC_000003.12 3 7283302 - GRM7 NNNTCACAAAAATGTTTACA NGG 2 0.3077 Tier II
5 NC_000014.9 14 90970460 + RPS6KA5 NNNTCACAAAACTGTCTACA NGG 2 0.2632 Tier II
6 NC_000015.10 15 40036089 + SRP14 NNNTCACAGAGATGTCTACA NAG 2 0.1728 Tier II
7 NC_000003.12 3 77369375 + ROBO2 NNNTCACAAAAATGTGTACA NGG 2 0.1538 Tier II
8 NC_000010.11 10 82374860 - NRG3 NNNTCACAAACATGTTTACA NGG 2 0.1319 Tier II
9 NC_000004.12 4 41136308 + APBB2 NNNTCACAAAGATGTTTAAA NGG 2 0.1319 Tier II
10 NC_000005.10 5 35769199 + SPEF2 NNNTCACAAAGTTGTTTACA NGG 2 0.1026 Tier II
11 NC_000023.11 X 18795461 - PPEF1 NNNTCACAAAGATGAGTACA NGG 2 0.0891 Tier II
12 NC_000012.12 12 5661161 + ANO2 NNNTCACAAAGACGTCTACA NCG 2 0.0846 Tier II
13 NC_000005.10 5 76433067 + IQGAP2 NNNTCACAAAGATGTGAACA NGG 2 0.0821 Tier II
14 NC_000005.10 5 108884872 + FER NNNTCACAATGATGTCTACA NTG 2 0.0344 Tier II
15 NC_000003.12 3 114727702 - ZBTB20 NNNTCACAAAGATTTCTACA NGA 2 0.0185 Tier II
16 NC_000023.11 X 24658808 - PCYT1B NNNTCCCAAAGATGTCTACA NTG 2 0.0177 Tier II
17 NC_000006.12 6 165417031 + PDE10A NNNTAACAAAGATGTCTACA NGT 2 0.0092 Tier II
18 NC_000019.10 19 21322097 - ZNF708 NNNTCACAAACATGTCTACA NGT 2 0.0069 Tier II
19 NC_000001.11 1 215135021 - KCNK2 NNNTCACAAAGTTGTCTACA NGT 2 0.0054 Tier II
20 NC_000002.12 2 228484870 - LINC01807 NNNTCACAAAGATGTCTAAT NGG 2 0.2571 Tier III
21 NC_000018.10 18 22881039 + RBBP8-AS1 NNNTCACCCAGATGTCTACA NGG 2 0.2449 Tier III
22 NC_000015.10 15 95074107 + LOC105370991 NNNGCACAAAGATTTCTACA NGG 2 0.1667 Tier III
23 NC_000020.11 20 3068684 + LOC101929098 NNNTCACAAAGAAGTCTACA NTG 2 0.027 Tier III
24 NC_000003.12 3 77960395 - LOC105377171 NNNTCACAAAGATGGCTTCA NGG 2 0.025 Tier III
25 NC_000011.10 11 41642218 - LINC02741 NNNTCAAAAAGATGTCTACA NGC 2 0.0167 Tier III
26 NC_000003.12 3 170699622 - SLC7A14-AS1 NNNTCCCAAAGATGTCTACA NGC 2 0.0101 Tier III
27 NC_000006.12 6 19032223 - LOC105374958 NNNTAACAAAGATGTCTACA NGT 2 0.0092 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 5249208 - Cdk14 NNNTCACAAAGATGTCTACC NGG 1 0.2273 Tier I
2 NC_000080.6 14 26718186 - Dnah12 NNNTCACATAGAAGTCTACA NGG 2 0.4154 Tier II
3 NC_000071.6 5 113128172 + 2900026A02Rik NNNTCACAGAGATGACTACA NGG 2 0.386 Tier II
4 NC_000069.6 3 105507315 - Kcnd3 NNNTCACAAATATGTCTACA NGG 1 0.3846 Tier II
5 NC_000084.6 18 14752373 + Psma8 NNNTCTCAAAGATGTCTTCA NGG 2 0.3571 Tier II
6 NC_000070.6 4 151405882 + Camta1 NNNTTACAAGGATGTCTACA NGG 2 0.3535 Tier II
7 NC_000075.6 9 115994926 - Gadl1 NNNTCACAAAGATGACAACA NGG 2 0.3088 Tier II
8 NC_000074.6 8 75622956 + Iqcm NNNTCACACAGATGTCAACA NGG 2 0.3048 Tier II
9 NC_000067.6 1 85170537 + Gm38510 NNNTCACAAATATATCTACA NGG 2 0.2885 Tier II
10 NC_000067.6 1 85497622 + Gm2427 NNNTCACAAATATATCTACA NGG 2 0.2885 Tier II
11 NC_000067.6 1 85497622 + Gm7592 NNNTCACAAATATATCTACA NGG 2 0.2885 Tier II
12 NC_000067.6 1 85627596 + Sp140 NNNTCACAAATATATCTACA NGG 2 0.2885 Tier II
13 NT_166280.1 1 35340 + LOC108167387 NNNTCACAAATATATCTACA NGG 2 0.2885 Tier II
14 NC_000075.6 9 23332472 - Bmper NNNTCACAAGGATGTCTTCA NGG 2 0.2778 Tier II
15 NC_000086.7 X 51417920 - Hs6st2 NNNCCACAAACATGTCTACA NGG 2 0.2773 Tier II
16 NC_000076.6 10 5859478 + Rgs17 NNNTCACATAGATGTCTATA NGG 2 0.2769 Tier II
17 NC_000071.6 5 73134793 - Fryl NNNTCACGAAGATGCCTACA NGG 2 0.2 Tier II
18 NC_000076.6 10 29334927 - Echdc1 NNNTCTCAAAGCTGTCTACA NGG 2 0.188 Tier II
19 NC_000082.6 16 4037306 - Dnase1 NNNTCACAGAGATGTCTACA NAG 2 0.1728 Tier II
20 NC_000081.6 15 8194271 + Cplane1 NNNTCACACAGCTGTCTACA NGG 2 0.1504 Tier II
21 NC_000080.6 14 122030535 + Timm8a2 NNNTCACAAAGATGTCAACA NAG 2 0.1383 Tier II
22 NC_000067.6 1 77393636 + Epha4 NNNTTACAAAGATGTCGACA NGG 2 0.0749 Tier II
23 NC_000078.6 12 78666655 - Gphn NNNTCACAAAGCTGTCTCCA NGG 2 0.0501 Tier II
24 NC_000076.6 10 119952035 + Grip1 NNNTCACAAAGATCTCTACA NGT 2 0.0069 Tier II
25 NC_000072.6 6 16467997 - Gm36406 NNNTCACAAAGATGTCAACA NGG 1 0.5333 Tier III
26 NC_000075.6 9 115994926 - Gm16142 NNNTCACAAAGATGACAACA NGG 2 0.3088 Tier III
27 NC_000067.6 1 85497622 + Gm2666 NNNTCACAAATATATCTACA NGG 2 0.2885 Tier III
28 NC_000078.6 12 5813413 + Gm9548 NNNCCACAAAGCTGTCTACA NGG 2 0.1703 Tier III
29 NC_000078.6 12 5813413 + Gm32442 NNNCCACAAAGCTGTCTACA NGG 2 0.1703 Tier III
30 NC_000070.6 4 103611198 + Gm36604 NNNTCACAAAGAAGTCTCCA NGG 2 0.1319 Tier III
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Other clones with same target sequence:

(none)