Construct: sgRNA BRDN0001147017
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGCTGGACAATGTAACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PSKH2 (85481)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77266
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
86064464 |
+ |
PSKH2 |
NNNGCTGGACAATGTAACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
27401680 |
- |
GABRG3 |
NNNGCTGGACAATCTAACTA |
NGG |
2 |
0.2857 |
Tier II |
| 3 |
NC_000015.10 |
15 |
28217875 |
+ |
HERC2 |
NNNGCTGGACAAAGTAAGGA |
NGG |
2 |
0.0923 |
Tier II |
| 4 |
NC_000002.12 |
2 |
40853490 |
- |
LOC105374497 |
NNNGCTGTTCAATGTAACGA |
NGG |
2 |
0.375 |
Tier III |
| 5 |
NC_000015.10 |
15 |
20442722 |
+ |
HERC2P3 |
NNNGCTGGACAAAGTAAGGA |
NGG |
2 |
0.0923 |
Tier III |
| 6 |
NC_000015.10 |
15 |
22557710 |
- |
HERC2P2 |
NNNGCTGGACAAAGTAAGGA |
NGG |
2 |
0.0923 |
Tier III |
| 7 |
NC_000013.11 |
13 |
50447491 |
- |
DLEU1 |
NNNGCTGGACAATGGAACGA |
NTG |
2 |
0.0019 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
89183623 |
+ |
Edil3 |
NNNACTGGACAATGTAATGA |
NGG |
2 |
0.5786 |
Tier II |
| 2 |
NC_000077.6 |
11 |
85381788 |
- |
Bcas3 |
NNNGCTTGACAATCTAACGA |
NGG |
2 |
0.2449 |
Tier II |
| 3 |
NC_000077.6 |
11 |
43026636 |
- |
Atp10b |
NNNGCTGGACAAGGTAACCA |
NGG |
2 |
0.1169 |
Tier II |
| 4 |
NC_000084.6 |
18 |
75872340 |
+ |
Zbtb7c |
NNNGCTGTACAATGTAAGGA |
NGG |
2 |
0.0833 |
Tier II |
| 5 |
NC_000079.6 |
13 |
108989016 |
- |
Pde4d |
NNNGCTGGACAATGTAGCCA |
NGG |
2 |
0.0791 |
Tier II |
| 6 |
NC_000085.6 |
19 |
57990275 |
+ |
Atrnl1 |
NNNGCTGGACAAGGTAGCGA |
NGG |
2 |
0.046 |
Tier II |
| 7 |
NC_000075.6 |
9 |
100450000 |
- |
Gm28166 |
NNNGCTGTACAATATAACGA |
NGG |
2 |
0.4688 |
Tier III |
| 8 |
NC_000078.6 |
12 |
49470128 |
+ |
Gm34381 |
NNNGCTGGACAATGTGATGA |
NGG |
2 |
0.1236 |
Tier III |
| 9 |
NC_000067.6 |
1 |
177578257 |
+ |
Gm30013 |
NNNGCTGGAGAATGTAGCGA |
NGG |
2 |
0.0686 |
Tier III |
Other clones with same target sequence:
(none)
