Construct: sgRNA BRDN0001147019

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGGGCAGAAGCTGCATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP4K2B (8396)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77903

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 38777742 - PIP4K2B NNNGGGCAGAAGCTGCATGT NGG 0 1.0 Tier I
2 NC_000019.10 19 38838900 - HNRNPL NNNGGGCAGAAGCTGAATGT NTG 2 0.039 Tier I
3 NC_000003.12 3 13034865 - IQSEC1 NNNGGACAGGAGCTGCATGT NGG 2 0.5556 Tier II
4 NC_000007.14 7 45715821 + ADCY1 NNNGGGTAGAGGCTGCATGT NGG 2 0.5281 Tier II
5 NC_000006.12 6 68854982 - ADGRB3 NNNGGTAAGAAGCTGCATGT NGG 2 0.5 Tier II
6 NC_000014.9 14 23173255 + SLC7A8 NNNGAGCAGAAGCTGCATGA NGG 2 0.4875 Tier II
7 NC_000001.11 1 237802816 - RYR2 NNNGGGCAGAAGCTGCATCT NGG 1 0.4483 Tier II
8 NC_000010.11 10 72069218 - SPOCK2 NNNGGGCAGAGGCTGCATTT NGG 2 0.4333 Tier II
9 NC_000002.12 2 69596839 + AAK1 NNNAGGCTGAAGCTGCATGT NGG 2 0.3857 Tier II
10 NC_000014.9 14 68593431 + RAD51B NNNGGGCAGAAGCTGCAGGT NGG 1 0.3333 Tier II
11 NC_000006.12 6 5303408 + FARS2 NNNGGGCTGAAGCTGCACGT NGG 2 0.1837 Tier II
12 NC_000009.12 9 127762718 - SH2D3C NNNGGGCAGAAGCTGACTGT NGG 2 0.1765 Tier II
13 NC_000011.10 11 133918071 + IGSF9B NNNGGGCAGAAGCTGCAGCT NGG 2 0.1494 Tier II
14 NC_000019.10 19 3386726 - NFIC NNNAGGCAGAAGCTGCTTGT NGG 2 0.12 Tier II
15 NC_000007.14 7 23745530 + STK31 NNNGGGCAGCAGCTGCAGGT NGG 2 0.1111 Tier II
16 NC_000022.11 22 33806081 - LARGE1 NNNGGGCAGAAGCTGCCTGA NGG 2 0.0993 Tier II
17 NC_000001.11 1 241655599 - WDR64 NNNGGGCAGAAGGTGCATGA NGG 2 0.0767 Tier II
18 NC_000020.11 20 34190854 - ASIP NNNGGGCAGAAGTTGCATGC NGG 2 0.0636 Tier II
19 NC_000006.12 6 69073282 + ADGRB3 NNNGGGCAGAAGCTGCAGGG NGG 2 0.0588 Tier II
20 NC_000009.12 9 129850624 - USP20 NNNAGGCAGAAGCTGCATGT NTG 2 0.0351 Tier II
21 NC_000020.11 20 53325422 + TSHZ2 NNNGGGAAGAAGCTGCATGT NTG 2 0.0292 Tier II
22 NC_000003.12 3 53074185 + RFT1 NNNGGGCAGAAGCTGGGTGT NGG 2 0.0271 Tier II
23 NC_000012.12 12 131786874 - SFSWAP NNNGGGCAGCAGCTGCATGT NGA 2 0.0231 Tier II
24 NC_000006.12 6 161140759 + AGPAT4 NNNGGGCAGAAGCTGCCTGC NGG 2 0.016 Tier II
25 NC_000015.10 15 42546536 - LRRC57 NNNGGCCAGAAGCTGCATGT NGT 2 0.011 Tier II
26 NC_000010.11 10 58615533 - BICC1 NNNGGGCACAAGCTGCATGT NGT 2 0.0087 Tier II
27 NC_000016.10 16 67331049 + LRRC36 NNNGGGCAGAAGGTGCATGT NTG 2 0.0053 Tier II
28 NC_000005.10 5 122722974 + LINC02201 NNNAGGTAGAAGCTGCATGT NGG 2 0.7313 Tier III
29 NC_000001.11 1 29459134 + LOC107984933 NNNGGGCAGTAGCAGCATGT NGG 2 0.5462 Tier III
30 NC_000001.11 1 193351906 - LINC01031 NNNGGGCAGAAGCTGCAATT NGG 2 0.4444 Tier III
31 NC_000006.12 6 7481508 - HNRNPLP1 NNNGGGCAGAAGCTGAATGT NTG 2 0.039 Tier III
32 NC_000007.14 7 77530068 - LOC124901681 NNNGGGCAGAAGCTGAATGT NTG 2 0.039 Tier III
33 NC_000015.10 15 34489419 - HNRNPLP2 NNNGGGCAGAAGCTGAATGT NTG 2 0.039 Tier III
34 NC_000019.10 19 38838900 - LOC124904712 NNNGGGCAGAAGCTGAATGT NTG 2 0.039 Tier III
35 NC_000015.10 15 93372208 + LOC105370982 NNNGGGCAGAAGCTGCTTGG NGG 2 0.0235 Tier III
36 NC_000006.12 6 161140759 + LOC124901455 NNNGGGCAGAAGCTGCCTGC NGG 2 0.016 Tier III
37 NC_000004.12 4 142946018 - USP38-DT NNNGGGCAGAAGCTGCATCT NGC 2 0.01 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 43545667 + Tln1 NNNGGGCAGAGGCTGCATGT NGG 1 0.65 Tier I
2 NC_000077.6 11 97722417 - Pip4k2b NNNGGGCAGAAGCTGCACGT NGG 1 0.4286 Tier I
3 NC_000082.6 16 38004628 - Gpr156 NNNGGGCAGAGGCTGCATCT NGG 2 0.2914 Tier I
4 NC_000072.6 6 86959250 - Aak1 NNNGGGCAGCAGCTGCCTGT NGG 2 0.0588 Tier I
5 NC_000074.6 8 122333868 - Zfpm1 NNNGGGCAGCAGCTGGATGT NGG 2 0.0513 Tier I
6 NC_000075.6 9 78087536 - Fbxo9 NNNGGGCAGAAGCTGCATGA NGC 2 0.0125 Tier I
7 NC_000068.7 2 21425169 + Gpr158 NNNGGGAAGAAGCTGAATGT NGG 2 0.75 Tier II
8 NC_000075.6 9 64566101 - Megf11 NNNAGGCAAAAGCTGCATGT NGG 2 0.5786 Tier II
9 NC_000084.6 18 68322243 - Rnmt NNNGGGCAGGAGCTGCATGT NGG 1 0.5556 Tier II
10 NC_000068.7 2 68348617 - Stk39 NNNGGGGAGAAGCTGAATGT NGG 2 0.4706 Tier II
11 NC_000068.7 2 9950635 - Taf3 NNNGGGCAGAGGCTGCACGT NGG 2 0.2786 Tier II
12 NC_000080.6 14 86763557 + Diaph3 NNNGGGCAGAAGCTGCCTGT NGG 1 0.1765 Tier II
13 NC_000074.6 8 45552808 - Sorbs2 NNNGGACAGAAGCTGCTTGT NGG 2 0.1333 Tier II
14 NC_000075.6 9 96870923 - Pxylp1 NNNAGGCAGAAGCTGCTTGT NGG 2 0.12 Tier II
15 NC_000075.6 9 42579281 + Grik4 NNNGGGCAGGAGCTGCCTGT NGG 2 0.098 Tier II
16 NC_000072.6 6 113861543 - Atp2b2 NNNGGGCACAAGCTGCCTGT NGG 2 0.095 Tier II
17 NC_000080.6 14 117049743 - Gpc6 NNNGGGCAGAAGCTGCAGGT NAG 2 0.0864 Tier II
18 NC_000074.6 8 108573174 - Zfhx3 NNNGGGCAGAAGCTACATGT NGA 2 0.0654 Tier II
19 NC_000079.6 13 12486447 + Edaradd NNNGTGCAGAAGCTGCGTGT NGG 2 0.0529 Tier II
20 NC_000077.6 11 83895873 + Hnf1b NNNGGGCAGATGCTTCATGT NGG 2 0.044 Tier II
21 NC_000080.6 14 35129633 + Grid1 NNNGGGCAGAAGCTGAATGT NTG 2 0.039 Tier II
22 NC_000084.6 18 35275421 - Sil1 NNNGGGCAGAAGCTTCATGC NGG 2 0.013 Tier II
23 NC_000085.6 19 7358688 - Spindoc NNNGGGCAGAAGCTGCATTT NGT 2 0.0108 Tier II
24 NC_000076.6 10 118523256 + Ifngas1 NNNGGGGAGAAGCTGAATGT NGG 2 0.4706 Tier III
25 NC_000078.6 12 112221269 + Gm36894 NNNGGGCACAGGCTGCATGT NGG 2 0.35 Tier III
26 NC_000075.6 9 100320502 - Gm28167 NNNGAGCAGAAGCTTCATGT NGG 2 0.1238 Tier III
27 NC_000074.6 8 108573174 - Lncbate1 NNNGGGCAGAAGCTACATGT NGA 2 0.0654 Tier III
28 NC_000074.6 8 108573174 - Gm51548 NNNGGGCAGAAGCTACATGT NGA 2 0.0654 Tier III
29 NC_000077.6 11 39687024 - Gm33963 NNNGGACAGAAGCTGCATGT NTG 2 0.039 Tier III
Download CSV

Other clones with same target sequence:

(none)