Construct: sgRNA BRDN0001147043

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AATGAGACAATACAGGAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPR2 (4882)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77754

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 35800096 + NPR2 NNNGAGACAATACAGGAAGG NGG 0 1.0 Tier I
2 NC_000003.12 3 112221201 - SLC9C1 NNNGAGACAATACAGGAATG NGA 2 0.0463 Tier I
3 NC_000005.10 5 136045599 + TGFBI NNNGAGACATAACAGGAAGG NGG 2 0.6618 Tier II
4 NC_000009.12 9 35806557 - NPR2 NNNAAGAAAATACAGGAAGG NGG 2 0.585 Tier II
5 NC_000003.12 3 40037811 - MYRIP NNNGAGAGAAAACAGGAAGG NGG 2 0.4821 Tier II
6 NC_000016.10 16 22828823 - HS3ST2 NNNGAGAAAATACAGGAAAG NGG 2 0.4643 Tier II
7 NC_000004.12 4 42015929 + SLC30A9 NNNCAGGCAATACAGGAAGG NGG 2 0.3737 Tier II
8 NC_000005.10 5 178914650 + ZFP2 NNNGAGACAATAAAGGAAGA NGG 2 0.3606 Tier II
9 NC_000007.14 7 15133381 + AGMO NNNGAGACAATAAAGGAAGA NGG 2 0.3606 Tier II
10 NC_000008.11 8 38286176 - NSD3 NNNGAGACACTACAGGAAGA NGG 2 0.3125 Tier II
11 NC_000008.11 8 4708918 + CSMD1 NNNGAGCAAATACAGGAAGG NGG 2 0.2844 Tier II
12 NC_000002.12 2 178224176 + OSBPL6 NNNGAGACAATAAAGGAAAG NGG 2 0.2747 Tier II
13 NC_000023.11 X 47024021 - JADE3 NNNGAGACAATACTGGATGG NGG 2 0.2667 Tier II
14 NC_000003.12 3 33001205 - GLB1 NNNGAGAGAAGACAGGAAGG NGG 2 0.2571 Tier II
15 NC_000004.12 4 146437027 - SLC10A7 NNNGAGAGAAGACAGGAAGG NGG 2 0.2571 Tier II
16 NC_000002.12 2 72220381 + EXOC6B NNNTAGAAAATACAGGAAGG NGG 2 0.2364 Tier II
17 NC_000014.9 14 64818076 + SPTB NNNGAGACAATGCAGGAAGG NAG 2 0.1872 Tier II
18 NC_000001.11 1 76541048 + ST6GALNAC3 NNNGAGAAAATACAGGAAGG NAG 2 0.1685 Tier II
19 NC_000018.10 18 4003689 + DLGAP1 NNNGAGAGAATACAGGAAGG NAG 2 0.1667 Tier II
20 NC_000023.11 X 124507359 - TENM1 NNNGAGAGAATACAGGAAGG NAG 2 0.1667 Tier II
21 NC_000012.12 12 69821838 - RAB3IP NNNGAGACAATCCAGGATGG NGG 2 0.1316 Tier II
22 NC_000005.10 5 177431736 + GRK6 NNNGAGACAATGCAGGGAGG NGG 2 0.1275 Tier II
23 NC_000016.10 16 22826997 + HS3ST2 NNNGAGAAAATACAGGGAGG NGG 2 0.1147 Tier II
24 NC_000005.10 5 141631651 + HDAC3 NNNGAGACAATACAGGAGGG NAG 2 0.1037 Tier II
25 NC_000005.10 5 42516604 + GHR NNNGAGACAATAGAGGAAAG NGG 2 0.0974 Tier II
26 NC_000002.12 2 74239840 - SLC4A5 NNNGAGACACTCCAGGAAGG NGG 2 0.0877 Tier II
27 NC_000011.10 11 24688931 - LUZP2 NNNGAGACAATACAGGGAGG NAG 2 0.0458 Tier II
28 NC_000023.11 X 130657512 + ENOX2 NNNGAGACAATAGAGGCAGG NGG 2 0.0241 Tier II
29 NC_000020.11 20 25499732 - NINL NNNTAGACAATACAGGAAGG NGC 2 0.0081 Tier II
30 NC_000007.14 7 102404333 - PRKRIP1 NNNGAGACAATACAGGAGGG NGT 2 0.0065 Tier II
31 NC_000020.11 20 445372 + TBC1D20 NNNGAGACAATACAGCAGGG NGG 2 0.0 Tier II
32 NC_000001.11 1 235157061 + RBM34 NNNGAGACAGTACAGTAAGG NGG 2 0.0 Tier II
33 NC_000007.14 7 47954541 + PKD1L1 NNNGGGACAATACAGCAAGG NGG 2 0.0 Tier II
34 NC_000011.10 11 6605144 + ILK NNNGTGACAATACAGCAAGG NGG 2 0.0 Tier II
35 NC_000001.11 1 209579562 + LOC107985256 NNNAAGACAATACAGAAAGG NGG 2 0.9 Tier III
36 NC_000004.12 4 154082721 + LOC101927947 NNNGAAACCATACAGGAAGG NGG 2 0.5714 Tier III
37 NC_000002.12 2 130541491 + PRSS40B NNNGAGCCAACACAGGAAGG NGG 2 0.2917 Tier III
38 NC_000002.12 2 130579243 - PRSS40A NNNGAGCCAACACAGGAAGG NGG 2 0.2917 Tier III
39 NC_000011.10 11 128222016 - LINC02098 NNNGAGACAATACAGGAGAG NGG 2 0.2857 Tier III
40 NC_000004.12 4 104664556 - CXXC4-AS1 NNNGAGCAAATACAGGAAGG NGG 2 0.2844 Tier III
41 NC_000003.12 3 141665729 + LRRC78P NNNGAGACAATGAAGGAAGG NGG 2 0.2778 Tier III
42 NC_000003.12 3 141665979 + LRRC78P NNNGAGACAATGAAGGAAGG NGG 2 0.2778 Tier III
43 NC_000006.12 6 22432184 + LOC105374971 NNNTGGACAATACAGGAAGG NGG 2 0.2618 Tier III
44 NC_000004.12 4 28238335 - LOC105374557 NNNGAGAAAAGACAGGAAGG NGG 2 0.26 Tier III
45 NC_000018.10 18 4003689 + DLGAP1-AS4 NNNGAGAGAATACAGGAAGG NAG 2 0.1667 Tier III
46 NC_000009.12 9 118122190 + LOC105376247 NNNGAGACAAGACAGGAGGG NGG 2 0.16 Tier III
47 NC_000007.14 7 8297732 - ICA1-AS1 NNNTAGACAATAAAGGAAGG NGG 2 0.1399 Tier III
48 NC_000008.11 8 20888516 + LOC105379315 NNNGAGACAAAACAGGAAGG NTG 2 0.0292 Tier III
49 NC_000012.12 12 119303060 + LINC00934 NNNGAGACAATGCAGGAAGG NTG 2 0.0281 Tier III
50 NC_000003.12 3 195697017 - MIR570HG NNNGAGACAATGCAGGAAGG NGC 2 0.016 Tier III
51 NC_000003.12 3 195697017 - LOC124909477 NNNGAGACAATGCAGGAAGG NGC 2 0.016 Tier III
52 NC_000016.10 16 69734433 - NQO1-DT NNNGAGACAATTCAGGAAGG NTG 2 0.013 Tier III
53 NC_000007.14 7 102404333 - LOC100630923 NNNGAGACAATACAGGAGGG NGT 2 0.0065 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 43640610 + Npr2 NNNGAGACTATACAGGAAGG NGG 1 0.6 Tier I
2 NC_000075.6 9 63980007 + Smad6 NNNGAAACAAAACAGGAAGG NGG 2 0.75 Tier II
3 NC_000077.6 11 74445970 + Rap1gap2 NNNGAAACAAAACAGGAAGG NGG 2 0.75 Tier II
4 NC_000076.6 10 33520006 + Clvs2 NNNGAGACAAAACAGGAAGA NGG 2 0.7031 Tier II
5 NC_000068.7 2 41035760 - Lrp1b NNNGAGACAATACAAGAATG NGG 2 0.6275 Tier II
6 NC_000078.6 12 85052526 + Ylpm1 NNNGAGACATTATAGGAAGG NGG 2 0.6176 Tier II
7 NC_000068.7 2 167617106 - Ube2v1 NNNGAGAGAATACAGGAAGA NGG 2 0.6027 Tier II
8 NC_000067.6 1 86214886 - Armc9 NNNAAGACAACACAGGAAGG NGG 2 0.6 Tier II
9 NC_000086.7 X 151367018 - Fam120c NNNCAAACAATACAGGAAGG NGG 2 0.5294 Tier II
10 NC_000071.6 5 35948673 + Afap1 NNNGAGGCAAAACAGGAAGG NGG 2 0.5294 Tier II
11 NC_000075.6 9 105805216 + Col6a6 NNNGAGACAATATAGGAAAG NGG 2 0.5 Tier II
12 NC_000085.6 19 4102220 - Pitpnm1 NNNGATACAAAACAGGAAGG NGG 2 0.5 Tier II
13 NC_000076.6 10 3880947 + Plekhg1 NNNGAGAAAAAACAGGAAGG NGG 2 0.4875 Tier II
14 NC_000074.6 8 120469011 + Gse1 NNNGGGACAACACAGGAAGG NGG 2 0.48 Tier II
15 NC_000077.6 11 103848418 + Nsf NNNGGGACAACACAGGAAGG NGG 2 0.48 Tier II
16 NC_000084.6 18 78008214 + Epg5 NNNGATACAATACAGGAAAG NGG 2 0.4762 Tier II
17 NC_000085.6 19 47006486 + Nt5c2 NNNGAGAGAATACAGGAAAG NGG 2 0.4592 Tier II
18 NC_000070.6 4 138291344 - Kif17 NNNGAGACAAGACAGGAAGG NGG 1 0.4 Tier II
19 NC_000072.6 6 93813033 + Magi1 NNNGAGACAAGACAGGAAGG NGG 1 0.4 Tier II
20 NC_000086.7 X 50899349 - Frmd7 NNNGAGAATATACAGGAAGG NGG 2 0.39 Tier II
21 NC_000074.6 8 99201033 + Cdh8 NNNGAGAAAGTACAGGAAGG NGG 2 0.3611 Tier II
22 NC_000085.6 19 48671589 - Sorcs3 NNNGAGACAATATGGGAAGG NGG 2 0.3267 Tier II
23 NC_000075.6 9 60079964 + Thsd4 NNNTAGACATTACAGGAAGG NGG 2 0.3209 Tier II
24 NC_000079.6 13 34010352 + Ripk1 NNNGAGACAAAACAGGAGGG NGG 2 0.3 Tier II
25 NC_000073.6 7 37526181 + Zfp536 NNNGAGACAAGACAGGAAAG NGG 2 0.2857 Tier II
26 NC_000068.7 2 35458662 - Ggta1 NNNGAGAGAAGACAGGAAGG NGG 2 0.2571 Tier II
27 NC_000073.6 7 24615583 - Phldb3 NNNGAGAGAAGACAGGAAGG NGG 2 0.2571 Tier II
28 NC_000077.6 11 104817019 - Gm11639 NNNGAGAGAAGACAGGAAGG NGG 2 0.2571 Tier II
29 NC_000068.7 2 148719445 - Napb NNNGAGACCATACAGGAAGC NGG 2 0.2449 Tier II
30 NC_000069.6 3 58582845 - Selenot NNNCAGACAAGACAGGAAGG NGG 2 0.2118 Tier II
31 NC_000080.6 14 60972287 + Tnfrsf19 NNNGAGACCATTCAGGAAGG NGG 2 0.1905 Tier II
32 NC_000075.6 9 21130930 - Tyk2 NNNGAGACGATCCAGGAAGG NGG 2 0.1754 Tier II
33 NC_000074.6 8 48692611 + Tenm3 NNNGAGAAAATACAGGAAGG NAG 2 0.1685 Tier II
34 NC_000077.6 11 107495898 - Psmd12 NNNGAGAGAATACAGGAAGG NAG 2 0.1667 Tier II
35 NC_000074.6 8 83183417 + Tbc1d9 NNNGAGACAATACAGGGAGA NGG 2 0.1654 Tier II
36 NC_000071.6 5 141389970 - Sdk1 NNNGTGACAAGACAGGAAGG NGG 2 0.1455 Tier II
37 NC_000068.7 2 155325884 + Pigu NNNGAGGCAATACAGGGAGG NGG 2 0.1246 Tier II
38 NC_000075.6 9 123112864 - Zdhhc3 NNNGAGACACTTCAGGAAGG NGG 2 0.1111 Tier II
39 NC_000077.6 11 98470192 + Ikzf3 NNNGAGACAAGACAGGAAGG NAG 2 0.1037 Tier II
40 NC_000071.6 5 117234916 + Taok3 NNNGAGACAATGGAGGAAGG NGG 2 0.0985 Tier II
41 NC_000078.6 12 57651058 - Ttc6 NNNGAGACAATTCACGAAGG NGG 2 0.0909 Tier II
42 NC_000076.6 10 105242192 + Tmtc2 NNNTAGACAATACAGGCAGG NGG 2 0.0642 Tier II
43 NC_000071.6 5 89119805 - Slc4a4 NNNGAGACAATAGAGGAGGG NGG 2 0.0545 Tier II
44 NC_000068.7 2 152761423 + Bcl2l1 NNNGAGACAATCCAGGCAGG NGG 2 0.0464 Tier II
45 NC_000068.7 2 152761423 + Cox4i2 NNNGAGACAATCCAGGCAGG NGG 2 0.0464 Tier II
46 NC_000078.6 12 4159053 - Adcy3 NNNGAGACAATACAGGGAGG NAG 2 0.0458 Tier II
47 NC_000078.6 12 75415235 + Gphb5 NNNGAGAAAATACAGGAAGG NGA 2 0.0451 Tier II
48 NC_000069.6 3 96900568 - Gpr89 NNNTAGACAATACAGGAAGG NGA 2 0.0253 Tier II
49 NC_000077.6 11 35130294 + Slit3 NNNGAGGCAATACAGGAAGG NGC 2 0.0157 Tier II
50 NC_000074.6 8 95295002 - Cngb1 NNNGACACAATACAGCAAGG NGG 2 0.0 Tier II
51 NC_000076.6 10 5302418 + Syne1 NNNGAGACAATAAAGTAAGG NGG 2 0.0 Tier II
52 NC_000068.7 2 172327680 + Gm14273 NNNGAGACAAGACAGAAAGG NGG 2 0.4 Tier III
53 NC_000072.6 6 31635426 + Gm31486 NNNGAGAGAAGACAGGAAGG NGG 2 0.2571 Tier III
54 NC_000069.6 3 30844281 + Gm42198 NNNGAGACATTACAGGGAGG NGG 2 0.1557 Tier III
55 NC_000075.6 9 113406911 + Gm36485 NNNGAGACAAGAAAGGAAGG NGG 2 0.1538 Tier III
56 NC_000072.6 6 98542128 + 4930595L18Rik NNNGAGAGAATACAGGCAGG NGG 2 0.1134 Tier III
57 NC_000069.6 3 82916750 - Rbm46os NNNGAGACAAGACAGGAAGG NAG 2 0.1037 Tier III
58 NC_000068.7 2 42886361 - Gm39803 NNNGAGAGAATAGAGGAAGG NGG 2 0.0877 Tier III
59 NC_000078.6 12 5390934 - 2810032G03Rik NNNGACACAATACAGGAAGG NGA 2 0.0473 Tier III
60 NC_000086.7 X 100746939 - Gm14902 NNNGAGACAATACAGGAAGG NCC 2 0.0 Tier III
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Other clones with same target sequence:

(none)