Construct: sgRNA BRDN0001147054
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTCACTCTAGATGAAATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CLK4 (57396)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77799
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
178616943 |
- |
CLK4 |
NNNCACTCTAGATGAAATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
27436394 |
- |
SLC4A7 |
NNNCACTCTAGATGAAATAG |
NAG |
2 |
0.1111 |
Tier I |
| 3 |
NC_000008.11 |
8 |
50551105 |
+ |
SNTG1 |
NNNCACTTTAGATGAAATTG |
NGG |
2 |
0.4038 |
Tier II |
| 4 |
NC_000011.10 |
11 |
79038961 |
- |
TENM4 |
NNNCACTCTAGATGTAATCC |
NGG |
2 |
0.0857 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
51273566 |
+ |
Clk4 |
NNNTACTTTAGATGAAATCG |
NGG |
2 |
0.7 |
Tier I |
| 2 |
NC_000080.6 |
14 |
54909298 |
- |
Slc22a17 |
NNNCACTCTAAATGAAATCC |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000084.6 |
18 |
63805425 |
- |
Wdr7 |
NNNCACTCTAGATGAAAAAG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000070.6 |
4 |
131973803 |
- |
Epb41 |
NNNCACTCTAGATGAAAGAG |
NGG |
2 |
0.1429 |
Tier II |
Other clones with same target sequence:
(none)
