Construct: sgRNA BRDN0001147069

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGCCTCATTTACTGAACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLK2 (11011)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75616

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 62565100 + TLK2 NNNCCTCATTTACTGAACAG NGG 0 1.0 Tier I
2 NC_000002.12 2 171049852 - TLK1 NNNCTTCATTTACTGAACAA NGG 2 0.5966 Tier I
3 NC_000010.11 10 37819921 - ZNF248 NNNCCTCGTTTACTGAACAA NGG 2 0.6875 Tier II
4 NC_000023.11 X 63763750 + ARHGEF9 NNNCCTCATTTACAAAACAG NGG 2 0.5826 Tier II
5 NC_000006.12 6 30945119 + MUCL3 NNNGCTCAATTACTGAACAG NGG 2 0.4643 Tier II
6 NC_000003.12 3 133883647 + RAB6B NNNCCTCTTTTACTGAACAG NGG 1 0.4286 Tier II
7 NC_000011.10 11 12517377 - PARVA NNNCAGCATTTACTGAACAG NGG 2 0.3265 Tier II
8 NC_000012.12 12 78945586 - SYT1 NNNTCTCATTTTCTGAACAG NGG 2 0.2667 Tier II
9 NC_000009.12 9 125591406 - MAPKAP1 NNNCCTCATTTCCTAAACAG NGG 2 0.2477 Tier II
10 NC_000005.10 5 50699033 - PARP8 NNNCCTCATTTCCTGAACAA NGG 2 0.2467 Tier II
11 NC_000003.12 3 45413059 + LARS2 NNNCCTCTTTTACTGAAAAG NGG 2 0.2308 Tier II
12 NC_000003.12 3 142774566 + TRPC1 NNNCCTCATTGACTGAACTG NGG 2 0.2154 Tier II
13 NC_000002.12 2 130521589 - CFC1B NNNCCTCCTTTAATGAACAG NGG 2 0.1648 Tier II
14 NC_000002.12 2 130599184 + CFC1 NNNCCTCCTTTAATGAACAG NGG 2 0.1648 Tier II
15 NC_000009.12 9 97336414 + CCDC180 NNNCATCATTTACTGAACAG NAG 2 0.1481 Tier II
16 NC_000004.12 4 5256510 - STK32B NNNGCTCATTTACTCAACAG NGG 2 0.1364 Tier II
17 NC_000023.11 X 107529681 + FRMPD3 NNNCCTCATTTATTGGACAG NGG 2 0.1346 Tier II
18 NC_000012.12 12 6768040 - PTMS NNNCCTCATTTACTCAACAC NGG 2 0.1169 Tier II
19 NC_000001.11 1 32316389 - HDAC1 NNNCCTCATTTACTAAACAG NGA 2 0.0654 Tier II
20 NC_000005.10 5 134816137 + DDX46 NNNCCTCATTTATTGAACAG NGA 2 0.0486 Tier II
21 NC_000017.11 17 20965634 + LOC124900389 NNNCATCATTTACTGAACAG NGA 2 0.0397 Tier II
22 NC_000013.11 13 48333544 - RB1 NNNCCTCATTTTCTGAACAG NTG 2 0.013 Tier II
23 NC_000015.10 15 65098681 - UBAP1L NNNCCTCATTCACTGTACAG NGG 2 0.0 Tier II
24 NC_000010.11 10 5710212 + TASOR2 NNNCCTCATTTACTGAACAG NAA 2 0.0 Tier II
25 NC_000010.11 10 37819921 - TLK2P2 NNNCCTCGTTTACTGAACAA NGG 2 0.6875 Tier III
26 NC_000003.12 3 77998280 - LOC105377171 NNNCCTAATTAACTGAACAG NGG 2 0.5625 Tier III
27 NC_000015.10 15 20944469 - LINC01193 NNNCCTAATTAACTGAACAG NGG 2 0.5625 Tier III
28 NC_000012.12 12 78945586 - LOC105369863 NNNTCTCATTTTCTGAACAG NGG 2 0.2667 Tier III
29 NC_000010.11 10 45114142 + RSU1P2 NNNCCTCATTCACTGAACAG NAG 2 0.1728 Tier III
30 NC_000009.12 9 97336414 + SUGT1P4-STRA6LP-CCDC180 NNNCATCATTTACTGAACAG NAG 2 0.1481 Tier III
31 NC_000023.11 X 107529681 + FRMPD3-AS1 NNNCCTCATTTATTGGACAG NGG 2 0.1346 Tier III
32 NC_000017.11 17 20965634 + LOC339260 NNNCATCATTTACTGAACAG NGA 2 0.0397 Tier III
33 NC_000021.9 21 38140738 - DSCR8 NNNCCTCATTTACTGCCCAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 105247558 + Tlk2 NNNCCTCGTTTACTGAGCAG NGG 2 0.1294 Tier I
2 NC_000068.7 2 25909674 - Kcnt1 NNNCCTCACTTACTGCACAG NGG 2 0.0 Tier I
3 NC_000080.6 14 45650345 - Ddhd1 NNNCCTAAATTACTGAACAG NGG 2 0.6964 Tier II
4 NC_000082.6 16 43798829 + Drd3 NNNCCTCACTTACTGAAAAG NGG 2 0.497 Tier II
5 NC_000075.6 9 117359170 + Rbms3 NNNCTTCATTTACTGAACAT NGG 2 0.4455 Tier II
6 NC_000071.6 5 104099107 + Sparcl1 NNNCTTCATCTACTGAACAG NGG 2 0.3394 Tier II
7 NC_000080.6 14 23525301 + Kcnma1 NNNCCTCATTTTCTAAACAG NGG 2 0.3137 Tier II
8 NC_000076.6 10 86448488 + Syn3 NNNCCTCATTTTCTGAACAA NGG 2 0.3125 Tier II
9 NC_000073.6 7 97007813 + Nars2 NNNACTCATTTACTCAACAG NGG 2 0.2297 Tier II
10 NC_000078.6 12 40455224 - Dock4 NNNTCTCATTTACTGAACAG NAG 2 0.2074 Tier II
11 NC_000074.6 8 61784510 + Palld NNNTCTCATTTACTGAGCAG NGG 2 0.1412 Tier II
12 NC_000069.6 3 100923770 - Trim45 NNNCCTCATTTACTAAAGAG NGG 2 0.1255 Tier II
13 NC_000072.6 6 116714562 + Tmem72 NNNCCTCATGTACTGGACAG NGG 2 0.0962 Tier II
14 NC_000077.6 11 57064573 + Gria1 NNNCCTCATTTACTGAGCAG NAG 2 0.0458 Tier II
15 NC_000080.6 14 10252263 + Fhit NNNCCTTATTTACTGAACAG NTG 2 0.0317 Tier II
16 NC_000073.6 7 126880764 + Taok2 NNNCCTCATTTACTGATGAG NGG 2 0.0178 Tier II
17 NC_000075.6 9 69816101 + B230323A14Rik NNNCTTCATTTACTGAACAA NGG 2 0.5966 Tier III
18 NC_000067.6 1 51919767 + Gm33795 NNNCCTCTTTTCCTGAACAG NGG 2 0.1128 Tier III
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Other clones with same target sequence:

(none)