Construct: sgRNA BRDN0001147084

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGATTGTTATGGAGTACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK3 (6788)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75975

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 98749325 - STK3 NNNATTGTTATGGAGTACTG NGG 0 1.0 Tier I
2 NC_000020.11 20 44981895 + STK4 NNNATCGTTATGGAGTACTG NGG 1 0.9091 Tier I
3 NC_000016.10 16 74682753 - MLKL NNNATTGTCATGGAGTACTG NGA 2 0.0641 Tier I
4 NC_000002.12 2 64095196 - PELI1 NNNATTGTTATGGCGTACTG NAG 2 0.0556 Tier I
5 NC_000002.12 2 101825333 + MAP4K4 NNNCTTGTTATGGAGTTCTG NGG 2 0.0471 Tier I
6 NC_000003.12 3 49420741 - AMT NNNATTGCTATGGAGTACTT NGG 2 0.7 Tier II
7 NC_000006.12 6 2681495 + MYLK4 NNNATTGTGAAGGAGTACTG NGG 2 0.4643 Tier II
8 NC_000003.12 3 122604343 + PARP15 NNNATTATTATGGAGTACTC NGG 2 0.4286 Tier II
9 NC_000023.11 X 134587659 - PLAC1 NNNATTGTTCTGGAGAACTG NGG 2 0.303 Tier II
10 NC_000007.14 7 22350150 + RAPGEF5 NNNATTGTTATGCAGTATTG NGG 2 0.2707 Tier II
11 NC_000009.12 9 101605875 - GRIN3A NNNGTTGTTATTGAGTACTG NGG 2 0.2404 Tier II
12 NC_000005.10 5 35102207 + PRLR NNNCTTTTTATGGAGTACTG NGG 2 0.2017 Tier II
13 NC_000006.12 6 46011194 + CLIC5 NNNATTGTTCTGGAGTCCTG NGG 2 0.0588 Tier II
14 NC_000003.12 3 122604343 + LOC124909419 NNNATTATTATGGAGTACTC NGG 2 0.4286 Tier III
15 NC_000018.10 18 10314303 - LOC105371985 NNNATTTTGATGGAGTACTG NGG 2 0.3537 Tier III
16 NC_000020.11 20 52622210 - LINC01524 NNNATTGTTCTGGAGTACAG NGG 2 0.0952 Tier III
17 NC_000020.11 20 52622210 - LOC105372666 NNNATTGTTCTGGAGTACAG NGG 2 0.0952 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 164086561 + Stk4 NNNATTGTTATGGAGTACTG NGG 0 1.0 Tier I
2 NC_000081.6 15 35099483 - Stk3 NNNATTGTTATGGAGTACTG NGG 0 1.0 Tier I
3 NC_000074.6 8 111319819 - Mlkl NNNATTGTCATGGAGTACTG NGA 2 0.0641 Tier I
4 NC_000067.6 1 86925628 + Dis3l2 NNNATAGTAATGGAGTACTG NGG 2 0.8048 Tier II
5 NC_000071.6 5 17790699 - Cd36 NNNTTTGTTATGGAATACTG NGG 2 0.5989 Tier II
6 NC_000078.6 12 101682758 - Tc2n NNNTTTGTTATGGAGCACTG NGG 2 0.4242 Tier II
7 NC_000080.6 14 21756342 - Samd8 NNNATTGTTTTGCAGTACTG NGG 2 0.3715 Tier II
8 NC_000079.6 13 81212512 - Adgrv1 NNNTTGGTTATGGAGTACTG NGG 2 0.3636 Tier II
9 NC_000076.6 10 123733943 - Tafa2 NNNCTTGTTATGGAGTACTG NGG 1 0.3529 Tier II
10 NC_000069.6 3 53042491 + Lhfp NNNAGTGTTATCGAGTACTG NGG 2 0.3388 Tier II
11 NC_000083.6 17 49843206 + Kif6 NNNATGGTTATGGAGGACTG NGG 2 0.1978 Tier II
12 NC_000081.6 15 97366954 + Pced1b NNNTTTGTTATGTAGTACTG NGG 2 0.1909 Tier II
13 NC_000076.6 10 108671373 - Syt1 NNNATTGTGATGGAGTGCTG NGG 2 0.1092 Tier II
14 NC_000077.6 11 66100268 - Dnah9 NNNATTGTTATGCAGTGCTG NGG 2 0.0743 Tier II
15 NC_000081.6 15 32329753 + Sema5a NNNATTGTTATGCAGTACTG NTG 2 0.0164 Tier II
16 NC_000073.6 7 60029804 - Snrpn NNNATTGTTATGGATTACTG NGC 2 0.0032 Tier II
17 NC_000067.6 1 86925628 + Gm41909 NNNATAGTAATGGAGTACTG NGG 2 0.8048 Tier III
18 NC_000073.6 7 60029804 - Snhg14 NNNATTGTTATGGATTACTG NGC 2 0.0032 Tier III
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Other clones with same target sequence:

(none)