Construct: sgRNA BRDN0001147093

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCTGGTGTCCCACAAAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C8orf44-SGK3 (100533105)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75907

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 66841094 - SGK3 NNNTGGTGTCCCACAAAATG NGG 0 1.0 Tier I
2 NC_000008.11 8 66841094 - C8orf44-SGK3 NNNTGGTGTCCCACAAAATG NGG 0 1.0 Tier I
3 NC_000007.14 7 147605345 + CNTNAP2 NNNTGGAGTCCCACAAAATA NGG 2 0.8203 Tier II
4 NC_000001.11 1 22524091 + ZBTB40 NNNTCGTGTCCCACAAAATG NGG 1 0.7857 Tier II
5 NC_000009.12 9 39190074 - CNTNAP3 NNNTGGTGTCCCACAAATTA NGG 2 0.4688 Tier II
6 NC_000009.12 9 61428904 + CNTNAP3C NNNTGGTGTCCCACAAATTA NGG 2 0.4688 Tier II
7 NC_000008.11 8 4638591 + CSMD1 NNNAGGTGTACCACAAAATG NGG 2 0.4127 Tier II
8 NC_000015.10 15 53762849 + WDR72 NNNTGGGGGCCCACAAAATG NGG 2 0.3641 Tier II
9 NC_000012.12 12 124553926 + NCOR2 NNNTGGTGTCCCTCAAAATG NGG 1 0.3 Tier II
10 NC_000003.12 3 171164272 + TNIK NNNTTGTGCCCCACAAAATG NGG 2 0.2769 Tier II
11 NC_000012.12 12 112052374 + NAA25 NNNTGCTGTGCCACAAAATG NGG 2 0.2652 Tier II
12 NC_000004.12 4 153758637 - RNF175 NNNTTGTGTACCACAAAATG NGG 2 0.26 Tier II
13 NC_000012.12 12 107442453 + ABTB3 NNNTTGTGTACCACAAAATG NGG 2 0.26 Tier II
14 NC_000017.11 17 33801119 + ASIC2 NNNTGGTGTCCATCAAAATG NGG 2 0.2143 Tier II
15 NC_000019.10 19 39385724 - PAF1 NNNTGGTTTCCCACAAAATG NAG 2 0.162 Tier II
16 NC_000019.10 19 39385724 - SAMD4B NNNTGGTTTCCCACAAAATG NAG 2 0.162 Tier II
17 NC_000007.14 7 117731657 + CTTNBP2 NNNTGGTGTGCCACAAAGTG NGG 2 0.1556 Tier II
18 NC_000009.12 9 136070554 - NACC2 NNNTGGTGTCCCACAAACTT NGG 2 0.1333 Tier II
19 NC_000002.12 2 219300833 + PTPRN NNNTGGTTTCCCCCAAAATG NGG 2 0.1316 Tier II
20 NC_000023.11 X 102285207 - NXF2 NNNTGGTGTCCCACAAAGAG NGG 2 0.1143 Tier II
21 NC_000023.11 X 102401911 + NXF2B NNNTGGTGTCCCACAAAGAG NGG 2 0.1143 Tier II
22 NC_000001.11 1 6076287 - KCNAB2 NNNTGGTGTCCTACAAACTG NGG 2 0.1026 Tier II
23 NC_000009.12 9 109999770 + PALM2AKAP2 NNNTTGTGTCCCACTAAATG NGG 2 0.06 Tier II
24 NC_000007.14 7 1062027 - C7orf50 NNNTGGTGTCTCACAAACTG NGG 2 0.0586 Tier II
25 NC_000002.12 2 217979428 - TNS1 NNNTGGTGTCCCACAAAATT NTG 2 0.0273 Tier II
26 NC_000013.11 13 100487687 - PCCA NNNTGGTATCCCACAAAATG NGC 2 0.0222 Tier II
27 NC_000004.12 4 3479372 - DOK7 NNNTGGTGGCCCACAAAATG NGT 2 0.01 Tier II
28 NC_000004.12 4 3479372 - LOC105374355 NNNTGGTGGCCCACAAAATG NGT 2 0.01 Tier II
29 NC_000012.12 12 103066883 - C12orf42 NNNTGGTGTCCCACAGAATG NGT 2 0.0031 Tier II
30 NC_000018.10 18 42793913 - RIT2 NNNGGGTGTCCCAGAAAATG NGG 2 0.0 Tier II
31 NC_000023.11 X 7332488 + STS NNNTTGTGTCCCAGAAAATG NGG 2 0.0 Tier II
32 NC_000007.14 7 142557646 - TRB NNNGGGTGCCCCACAAAATG NGG 2 0.5769 Tier III
33 NC_000022.11 22 47689657 - EPIC1 NNNTGGTGTATCACAAAATG NGG 2 0.2667 Tier III
34 NC_000012.12 12 112052374 + LOC124903022 NNNTGCTGTGCCACAAAATG NGG 2 0.2652 Tier III
35 NC_000002.12 2 122988547 + LOC105373596 NNNTGCTGTCCCACAAAAAG NGG 2 0.1948 Tier III
36 NC_000003.12 3 25896970 - LOC124909357 NNNTAGTGTCCCACAAAATG NGA 2 0.0602 Tier III
37 NC_000002.12 2 217979428 - TNS1-AS1 NNNTGGTGTCCCACAAAATT NTG 2 0.0273 Tier III
38 NC_000015.10 15 87459985 + LOC102724465 NNNTTGTGTCCCACAAAATG NTG 2 0.0117 Tier III
39 NC_000014.9 14 21783257 - TRA NNNTGGGGTCCCAGAAAATG NGG 2 0.0 Tier III
40 NC_000014.9 14 21783257 - TRAV7 NNNTGGGGTCCCAGAAAATG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 6542670 + Slc22a12 NNNTGGTGACCCACAAAATG NGG 1 0.9286 Tier I
2 NC_000067.6 1 156091911 - Fam163a NNNTGGTCTCCCACAAAATG NGG 1 0.6154 Tier II
3 NC_000067.6 1 151985355 + 1700025G04Rik NNNTGGTCTCCCACAAAATA NGG 2 0.5769 Tier II
4 NC_000081.6 15 43044996 + Rspo2 NNNTCGTGTCCAACAAAATG NGG 2 0.5612 Tier II
5 NC_000068.7 2 139841155 + Tasp1 NNNAGGTGGCCCACAAAATG NGG 2 0.2948 Tier II
6 NC_000068.7 2 41903406 - Lrp1b NNNTGATGTCCCACAAAAGG NGG 2 0.25 Tier II
7 NC_000078.6 12 108375535 + Eml1 NNNTGGTCTCCCACAAAATG NAG 2 0.1595 Tier II
8 NC_000077.6 11 77479793 + Ankrd13b NNNTGGAGTCCCACAACATG NGG 2 0.1544 Tier II
9 NC_000073.6 7 14114926 + Sult2a3 NNNTGGTGTCCAACAGAATG NGG 2 0.1374 Tier II
10 NC_000076.6 10 70453500 + Fam13c NNNTGGTGTCACACAACATG NGG 2 0.1324 Tier II
11 NC_000067.6 1 44662377 + Gulp1 NNNTGGGGTCCCACAACATG NGG 2 0.1038 Tier II
12 NC_000070.6 4 46798219 + Gabbr2 NNNTGGTGTCCCACAAGTTG NGG 2 0.0882 Tier II
13 NC_000082.6 16 24666641 + Lpp NNNTAGTGTCCCACAAAATG NTG 2 0.0338 Tier II
14 NC_000079.6 13 57610554 + Spock1 NNNAGGTGTCCCACAAAATG NTG 2 0.0186 Tier II
15 NC_000078.6 12 5121102 + Klhl29 NNNTGGTGACCCACAAAATG NGT 2 0.015 Tier II
16 NC_000067.6 1 182329488 - Fbxo28 NNNTGGTGACCCACATAATG NGG 2 0.0 Tier II
17 NC_000085.6 19 35875446 - A830019P07Rik NNNTGGTGTCTTACAAAATG NGG 2 0.1657 Tier III
18 NC_000080.6 14 70796909 + Gm34442 NNNTTGTGTCCCACAAAATG NAG 2 0.0778 Tier III
19 NC_000081.6 15 102271136 + Gm9918 NNNTTGTGTCCCACAAAAGG NGG 2 0.075 Tier III
20 NC_000072.6 6 42934215 + Olrf445-ps1 NNNTGGTGTGCCACAACATG NGG 2 0.0686 Tier III
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Other clones with same target sequence:

(none)