Construct: sgRNA BRDN0001147095
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAATCTTGAACGTCATGTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80222
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
19095564 |
- |
UBR4 |
NNNACCTTGAACGTCATGCT |
NGG |
2 |
0.2759 |
Tier I |
| 2 |
NC_000005.10 |
5 |
158939575 |
+ |
EBF1 |
NNNATCTTGAATGTCAAGTT |
NGG |
2 |
0.2872 |
Tier II |
| 3 |
NC_000003.12 |
3 |
24981277 |
+ |
RARB |
NNNATCTTGAACGCCATGTT |
NGG |
1 |
0.2857 |
Tier II |
| 4 |
NC_000012.12 |
12 |
76459833 |
- |
OSBPL8 |
NNNAGCTTGAACGGCATGTT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000012.12 |
12 |
15879267 |
+ |
LOC102724146 |
NNNATATTGAACATCATGTT |
NGG |
2 |
0.8571 |
Tier III |
| 6 |
NC_000011.10 |
11 |
109754133 |
+ |
LINC02715 |
NNNATCTTGAAAGTCATGTT |
NGG |
1 |
0.7143 |
Tier III |
| 7 |
NC_000002.12 |
2 |
220372612 |
+ |
LOC105373893 |
NNNATCTTGAACATCATGTG |
NGG |
2 |
0.1629 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
21994875 |
+ |
Ccdc151 |
NNNCTCTTGAACCTCATGTT |
NGG |
2 |
0.1486 |
Tier I |
| 2 |
NC_000085.6 |
19 |
29450728 |
+ |
Pdcd1lg2 |
NNNATAGTGAACGTCATGTT |
NGG |
2 |
0.5462 |
Tier II |
| 3 |
NC_000076.6 |
10 |
77583457 |
- |
Pttg1ip |
NNNATCTCGAATGTCATGTT |
NGG |
2 |
0.5385 |
Tier II |
| 4 |
NC_000077.6 |
11 |
62291106 |
+ |
Ttc19 |
NNNATCTTGAACTTCAAGTT |
NGG |
2 |
0.16 |
Tier II |
| 5 |
NC_000067.6 |
1 |
172613531 |
- |
Fcrl6 |
NNNATCTTGAACCTCATGTT |
NTG |
2 |
0.0164 |
Tier II |
Other clones with same target sequence:
(none)
