Construct: sgRNA BRDN0001147104
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCTGGGGTGGAGCGCACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GCK (2645)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77583
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
44153325 |
+ |
GCK |
NNNTGGGGTGGAGCGCACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
102080882 |
- |
HSP90AA1 |
NNNTGGGGTGGAGCACACGT |
NGG |
1 |
0.9412 |
Tier II |
| 3 |
NC_000011.10 |
11 |
17463251 |
+ |
ABCC8 |
NNNTGGGGTAGAGCACACGT |
NGG |
2 |
0.8784 |
Tier II |
| 4 |
NC_000008.11 |
8 |
96530210 |
+ |
SDC2 |
NNNTGGGGTGGAGTGCATGT |
NGG |
2 |
0.4714 |
Tier II |
| 5 |
NC_000009.12 |
9 |
136397636 |
+ |
SNAPC4 |
NNNTGGGGTGGGGAGCACGT |
NGG |
2 |
0.2528 |
Tier II |
| 6 |
NC_000009.12 |
9 |
136397700 |
+ |
SNAPC4 |
NNNTGGGGTGGGGAGCACGT |
NGG |
2 |
0.2528 |
Tier II |
| 7 |
NC_000009.12 |
9 |
136397716 |
+ |
SNAPC4 |
NNNTGGGGTGGGGAGCACGT |
NGG |
2 |
0.2528 |
Tier II |
| 8 |
NC_000009.12 |
9 |
136397748 |
+ |
SNAPC4 |
NNNTGGGGTGGGGAGCACGT |
NGG |
2 |
0.2528 |
Tier II |
| 9 |
NC_000008.11 |
8 |
27977178 |
- |
SCARA5 |
NNNGGGGGTGGAGCCCACGT |
NGG |
2 |
0.1705 |
Tier II |
| 10 |
NC_000022.11 |
22 |
28923894 |
+ |
ZNRF3 |
NNNTGGGGTGGAGCCCAAGT |
NGG |
2 |
0.1469 |
Tier II |
| 11 |
NC_000002.12 |
2 |
229701732 |
- |
DNER |
NNNTGGGGTGGGGCTCACGT |
NGG |
2 |
0.1032 |
Tier II |
| 12 |
NC_000001.11 |
1 |
54008104 |
+ |
LDLRAD1 |
NNNTGGGGTGGAGAGCAGGT |
NGG |
2 |
0.0467 |
Tier II |
| 13 |
NC_000019.10 |
19 |
38607223 |
+ |
MAP4K1 |
NNNTGGGGTGGAGAGCAGGT |
NGG |
2 |
0.0467 |
Tier II |
| 14 |
NC_000006.12 |
6 |
159989314 |
+ |
IGF2R |
NNNTGGGGAGGAGGGCACGT |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000009.12 |
9 |
34512300 |
- |
DNAI1 |
NNNTGGGGGGGAGGGCACGT |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000016.10 |
16 |
27884060 |
- |
GSG1L |
NNNTGGGGTGGAGGGCACGT |
NGC |
2 |
0.0 |
Tier II |
| 17 |
NC_000007.14 |
7 |
31166993 |
+ |
LOC107986781 |
NNNTGGGGTGGAGCACACAT |
NGG |
2 |
0.6723 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
5910886 |
+ |
Gck |
NNNTGGGGTGGAACGCACGT |
NGG |
1 |
0.9231 |
Tier I |
| 2 |
NC_000070.6 |
4 |
130074557 |
+ |
Col16a1 |
NNNTGGGGAGGAGCACACGT |
NGG |
2 |
0.8739 |
Tier II |
| 3 |
NC_000072.6 |
6 |
93834276 |
+ |
Magi1 |
NNNTGGGGTGCAGCTCACGT |
NGG |
2 |
0.0612 |
Tier II |
Other clones with same target sequence:
(none)
