Construct: sgRNA BRDN0001147109

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGAAAACATTTCACCCCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKACB (5567)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75961

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 84185142 - PRKACB NNNGAAAACATTTCACCCCC NGG 0 1.0 Tier I
2 NC_000005.10 5 178603763 - CLK4 NNNAAAAACATTTCTCCCCC NGG 2 0.18 Tier I
3 NC_000001.11 1 116642912 + IGSF3 NNNGAAAATATTTCACCACC NGG 2 0.4712 Tier II
4 NC_000007.14 7 777219 + DNAAF5 NNNGAAACCATCTCACCCCC NGG 2 0.406 Tier II
5 NC_000004.12 4 6400038 - PPP2R2C NNNGAAAACATTTCAACCCC NAG 2 0.2593 Tier II
6 NC_000010.11 10 115910682 + ATRNL1 NNNGAAAACATTTCACTCAC NGG 2 0.2 Tier II
7 NC_000002.12 2 187447682 - CALCRL NNNGAAAACATTTCTCCCCC NGG 1 0.2 Tier II
8 NC_000005.10 5 170054033 - DOCK2 NNNGAAAACAATTCACCCCC NAG 2 0.1944 Tier II
9 NC_000003.12 3 169244210 + MECOM NNNTAAAACATTTCACCCAC NGG 2 0.1558 Tier II
10 NC_000002.12 2 27842619 + RBKS NNNGAAAACATTTCACCACC NAG 2 0.1396 Tier II
11 NC_000010.11 10 99033236 - HPSE2 NNNGAAAACATTTCACCACC NAG 2 0.1396 Tier II
12 NC_000003.12 3 84960241 - CADM2 NNNGAACACATTTCCCCCCC NGG 2 0.1193 Tier II
13 NC_000015.10 15 65961937 + MEGF11 NNNGAAAACATTTCCCCCAC NGG 2 0.1169 Tier II
14 NC_000004.12 4 139127175 + ELF2 NNNGAACACATTTCACCCCC NAG 2 0.1134 Tier II
15 NC_000010.11 10 51838617 - PRKG1 NNNGAAGACATTTCAGCCCC NGG 2 0.1086 Tier II
16 NC_000018.10 18 74287891 + CYB5A NNNGCAAACATTTCTCCCCC NGG 2 0.1 Tier II
17 NC_000007.14 7 141303021 - TMEM178B NNNGACAACATTTCTCCCCC NGG 2 0.0909 Tier II
18 NC_000014.9 14 93490141 + UNC79 NNNGAAACCATTTCTCCCCC NGG 2 0.0857 Tier II
19 NC_000008.11 8 104573584 + LRP12 NNNGAAAACATTTCTCCCCT NGG 2 0.06 Tier II
20 NC_000010.11 10 95865525 + ENTPD1 NNNGAAAACATTTTACCCCC NGT 2 0.0118 Tier II
21 NC_000003.12 3 7630787 + GRM7 NNNGAAAACAGTTGACCCCC NGG 2 0.0 Tier II
22 NC_000022.11 22 21255446 + GGT2P NNNGAAAATATTTCACCACC NGG 2 0.4712 Tier III
23 NC_000003.12 3 55159260 - LOC124906243 NNNAAAAACATTTCACCCTC NGG 2 0.4154 Tier III
24 NC_000010.11 10 85570555 + LOC105378404 NNNAAAAACATTTCACCCAC NGG 2 0.3857 Tier III
25 NC_000010.11 10 115910682 + LOC105378497 NNNGAAAACATTTCACTCAC NGG 2 0.2 Tier III
26 NC_000002.12 2 187447682 - CALCRL-AS1 NNNGAAAACATTTCTCCCCC NGG 1 0.2 Tier III
27 NC_000003.12 3 169244210 + LOC124909454 NNNTAAAACATTTCACCCAC NGG 2 0.1558 Tier III
28 NC_000006.12 6 113189482 - LOC105377953 NNNGAAAACATTTCACTCCT NGG 2 0.14 Tier III
29 NC_000006.12 6 113189482 - LOC105377955 NNNGAAAACATTTCACTCCT NGG 2 0.14 Tier III
30 NC_000002.12 2 18391314 - LOC105373454 NNNGAGAACATTTCACCCCC NGC 2 0.0159 Tier III
31 NC_000010.11 10 95865525 + ENTPD1-AS1 NNNGAAAACATTTTACCCCC NGT 2 0.0118 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 102804117 - Mast4 NNNGATAACATTTCAACCCC NGG 2 0.7143 Tier II
2 NC_000086.7 X 82870240 - Dmd NNNGAAAACATCTTACCCCC NGG 2 0.6947 Tier II
3 NC_000083.6 17 52791265 - Kcnh8 NNNGAAAACATGTCACCCCC NGG 1 0.5 Tier II
4 NC_000068.7 2 166712195 + Prex1 NNNGAAAACTTTTCCCCCCC NGG 2 0.2406 Tier II
5 NC_000074.6 8 45315171 + Cyp4v3 NNNGCAAACATTTCACTCCC NGG 2 0.2333 Tier II
6 NC_000081.6 15 58341793 - Anxa13 NNNGAAAACCCTTCACCCCC NGG 2 0.2222 Tier II
7 NC_000086.7 X 24558194 + Gm4297 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
8 NC_000086.7 X 25628130 + Gm2003 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
9 NC_000086.7 X 26528403 + Slx NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
10 NC_000086.7 X 26685522 + Gm14525 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
11 NC_000086.7 X 26918490 + Gm6121 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
12 NC_000086.7 X 27477780 + Gm10230 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
13 NC_000086.7 X 27929765 + Gm10058 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
14 NC_000086.7 X 28276438 + Gm4836 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
15 NC_000086.7 X 28590738 + Gm10147 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
16 NC_000086.7 X 28937553 + Gm10096 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
17 NC_000086.7 X 29993436 - Gm10488 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
18 NC_000086.7 X 30346900 - Gm14632 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
19 NC_000086.7 X 30826270 + Gm10487 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
20 NC_000086.7 X 35084576 - Gm10486 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
21 NC_000086.7 X 35421347 - Gm14819 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier II
22 NC_000075.6 9 108067281 + Rnf123 NNNGAAAACCTTTCACCCCA NGG 2 0.1667 Tier II
23 NC_000069.6 3 30108350 + Mecom NNNTAAAACATTTCACCCAC NGG 2 0.1558 Tier II
24 NC_000082.6 16 97792578 + Prdm15 NNNGAAAACAGTTCACCCCT NGG 2 0.12 Tier II
25 NC_000082.6 16 11624195 - Snx29 NNNGAAAACATTTCACCCTC NAG 2 0.1197 Tier II
26 NC_000081.6 15 67148008 + St3gal1 NNNGAAAACATTACAGCCCC NGG 2 0.1065 Tier II
27 NC_000083.6 17 65388325 - Tmem232 NNNGAACACATTTCACCCGC NGG 2 0.0547 Tier II
28 NC_000082.6 16 6704418 - Rbfox1 NNNGGAAACATTTGACCCCC NGG 2 0.0 Tier II
29 NC_000077.6 11 94875447 + A430060F13Rik NNNGAAAACATGTCACCTCC NGG 2 0.3214 Tier III
30 NC_000074.6 8 45315171 + Gm30504 NNNGCAAACATTTCACTCCC NGG 2 0.2333 Tier III
31 NC_000086.7 X 24954198 + Gm2759 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier III
32 NC_000086.7 X 26740686 + Gm6113 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier III
33 NC_000086.7 X 27582822 + Gm2092 NNNGAAAACGTTTAACCCCC NGG 2 0.1944 Tier III
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Other clones with same target sequence:

(none)