Construct: sgRNA BRDN0001147113
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGACGAAGATGACTGACAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAST3 (23031)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76105
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
18143795 |
- |
MAST3 |
NNNCGAAGATGACTGACAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
131542702 |
+ |
CPNE4 |
NNNCGATGATGACTGACATG |
NGG |
2 |
0.2917 |
Tier I |
| 3 |
NC_000012.12 |
12 |
11770023 |
+ |
ETV6 |
NNNGCAAGATGACTGACAGG |
NGG |
2 |
0.3929 |
Tier II |
| 4 |
NC_000009.12 |
9 |
114424009 |
+ |
WHRN |
NNNTGAAGATGACTGACAGC |
NGG |
2 |
0.3429 |
Tier II |
| 5 |
NC_000010.11 |
10 |
50250663 |
+ |
ASAH2 |
NNNCCAAGATGACTGACAGC |
NGG |
2 |
0.3367 |
Tier II |
| 6 |
NC_000001.11 |
1 |
205521007 |
+ |
CDK18 |
NNNTGAAGATGACTGACAGG |
NAG |
2 |
0.2074 |
Tier II |
| 7 |
NC_000002.12 |
2 |
167212227 |
- |
XIRP2 |
NNNCGAAGAAGACTGGCAGG |
NGG |
2 |
0.1648 |
Tier II |
| 8 |
NC_000001.11 |
1 |
103508351 |
- |
RNPC3-DT |
NNNCCAAGATGACTGACAGT |
NGG |
2 |
0.55 |
Tier III |
| 9 |
NC_000018.10 |
18 |
27593055 |
+ |
LOC107985126 |
NNNCGAAGATGACTTACAGG |
NAG |
2 |
0.037 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
105026795 |
- |
Cpne4 |
NNNCGATGATGACTGACATG |
NGG |
2 |
0.2917 |
Tier I |
| 2 |
NC_000072.6 |
6 |
90391432 |
+ |
Zxdc |
NNNTGAAGATAACTGACAGG |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000083.6 |
17 |
85162051 |
+ |
Camkmt |
NNNGGAAAATGACTGACAGG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000084.6 |
18 |
68239585 |
- |
Ldlrad4 |
NNNCTCAGATGACTGACAGG |
NGG |
2 |
0.1364 |
Tier II |
| 5 |
NC_000072.6 |
6 |
50787694 |
- |
C530044C16Rik |
NNNAGAAGATGACTGACAGG |
NGG |
1 |
0.8421 |
Tier III |
| 6 |
NC_000083.6 |
17 |
85162051 |
+ |
Gm36826 |
NNNGGAAAATGACTGACAGG |
NGG |
2 |
0.5 |
Tier III |
| 7 |
NC_000084.6 |
18 |
39811488 |
+ |
Gm41707 |
NNNTTAAGATGACTGACAGG |
NGG |
2 |
0.24 |
Tier III |
| 8 |
NC_000078.6 |
12 |
97360956 |
+ |
Gm40552 |
NNNCCAAGATGACTGTCAGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
