Construct: sgRNA BRDN0001147113

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGACGAAGATGACTGACAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST3 (23031)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76105

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 18143795 - MAST3 NNNCGAAGATGACTGACAGG NGG 0 1.0 Tier I
2 NC_000003.12 3 131542702 + CPNE4 NNNCGATGATGACTGACATG NGG 2 0.2917 Tier I
3 NC_000012.12 12 11770023 + ETV6 NNNGCAAGATGACTGACAGG NGG 2 0.3929 Tier II
4 NC_000009.12 9 114424009 + WHRN NNNTGAAGATGACTGACAGC NGG 2 0.3429 Tier II
5 NC_000010.11 10 50250663 + ASAH2 NNNCCAAGATGACTGACAGC NGG 2 0.3367 Tier II
6 NC_000001.11 1 205521007 + CDK18 NNNTGAAGATGACTGACAGG NAG 2 0.2074 Tier II
7 NC_000002.12 2 167212227 - XIRP2 NNNCGAAGAAGACTGGCAGG NGG 2 0.1648 Tier II
8 NC_000001.11 1 103508351 - RNPC3-DT NNNCCAAGATGACTGACAGT NGG 2 0.55 Tier III
9 NC_000018.10 18 27593055 + LOC107985126 NNNCGAAGATGACTTACAGG NAG 2 0.037 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 105026795 - Cpne4 NNNCGATGATGACTGACATG NGG 2 0.2917 Tier I
2 NC_000072.6 6 90391432 + Zxdc NNNTGAAGATAACTGACAGG NGG 2 0.8 Tier II
3 NC_000083.6 17 85162051 + Camkmt NNNGGAAAATGACTGACAGG NGG 2 0.5 Tier II
4 NC_000084.6 18 68239585 - Ldlrad4 NNNCTCAGATGACTGACAGG NGG 2 0.1364 Tier II
5 NC_000072.6 6 50787694 - C530044C16Rik NNNAGAAGATGACTGACAGG NGG 1 0.8421 Tier III
6 NC_000083.6 17 85162051 + Gm36826 NNNGGAAAATGACTGACAGG NGG 2 0.5 Tier III
7 NC_000084.6 18 39811488 + Gm41707 NNNTTAAGATGACTGACAGG NGG 2 0.24 Tier III
8 NC_000078.6 12 97360956 + Gm40552 NNNCCAAGATGACTGTCAGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)