Construct: sgRNA BRDN0001147114
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGAGCCTCGTTATATCCTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRGUK (136332)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78085
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
134199266 |
+ |
LRGUK |
NNNGCCTCGTTATATCCTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
3426602 |
- |
PRMT8 |
NNNGCCTTGTTATATCCTGT |
NGG |
2 |
0.6125 |
Tier II |
| 3 |
NC_000011.10 |
11 |
33390263 |
- |
KIAA1549L |
NNNGCCTCTTAATATCCTGG |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000010.11 |
10 |
14985293 |
+ |
MEIG1 |
NNNGACTCGTCATATCCTGG |
NGG |
2 |
0.381 |
Tier II |
| 5 |
NC_000002.12 |
2 |
231266201 |
+ |
ARMC9 |
NNNGCATCGTTATCTCCTGG |
NGG |
2 |
0.199 |
Tier II |
| 6 |
NC_000012.12 |
12 |
121288836 |
- |
CAMKK2 |
NNNGCCTCTTTTTATCCTGG |
NGG |
2 |
0.1778 |
Tier II |
| 7 |
NC_000003.12 |
3 |
125144537 |
+ |
SLC12A8 |
NNNGCCTCCTTATCTCCTGG |
NGG |
2 |
0.1154 |
Tier II |
| 8 |
NC_000012.12 |
12 |
3426602 |
- |
LOC105369607 |
NNNGCCTTGTTATATCCTGT |
NGG |
2 |
0.6125 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
34092783 |
+ |
Lrguk |
NNNGCCTCGATATATCTTGG |
NGG |
2 |
0.4 |
Tier I |
| 2 |
NC_000084.6 |
18 |
21548749 |
- |
Gm31646 |
NNNGCCTGATTATATCCTGG |
NGG |
2 |
0.4133 |
Tier III |
Other clones with same target sequence:
(none)
