Construct: sgRNA BRDN0001147115

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGATGGGTGTGATGCATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAK5 (57144)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75714

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 9644159 + PAK5 NNNTGGGTGTGATGCATGAA NGG 0 1.0 Tier I
2 NC_000014.9 14 88483574 - PTPN21 NNNAGGGAGTGATGCATGAA NGG 2 0.381 Tier II
3 NC_000007.14 7 47325783 - TNS3 NNNTGGGTGTGATGCATGGA NGG 1 0.375 Tier II
4 NC_000013.11 13 28244390 + PAN3 NNNTGGGTGTAATGAATGAA NGG 2 0.2222 Tier II
5 NC_000003.12 3 119368560 + ARHGAP31 NNNTGGGTGGGATGCATGGA NGG 2 0.1875 Tier II
6 NC_000012.12 12 132217238 - GALNT9 NNNTGGGTGGGATGCATGGA NGG 2 0.1875 Tier II
7 NC_000016.10 16 11639453 + LITAF NNNTGGGTGGGATGCATGGA NGG 2 0.1875 Tier II
8 NC_000016.10 16 4677720 + MGRN1 NNNTGGCTGTGAGGCATGAA NGG 2 0.1793 Tier II
9 NC_000015.10 15 92104606 + SLCO3A1 NNNTGGGGGTGATGAATGAA NGG 2 0.163 Tier II
10 NC_000024.10 Y 12770835 + USP9Y NNNTGTGTGTGATGAATGAA NGG 2 0.1481 Tier II
11 NC_000002.12 2 112647770 - SLC20A1 NNNTGGGTGTGCTCCATGAA NGG 2 0.1128 Tier II
12 NC_000011.10 11 44912797 + TSPAN18 NNNTGGGTGTTATGCATGCA NGG 2 0.0796 Tier II
13 NC_000016.10 16 82965863 - CDH13 NNNTGGGTGTGATGTATGTA NGG 2 0.0359 Tier II
14 NC_000005.10 5 34698418 + RAI14 NNNTGTGTGTGATGGATGAA NGG 2 0.0333 Tier II
15 NC_000022.11 22 45294817 - UPK3A NNNTGGGTGTGAGGCAGGAA NGG 2 0.0307 Tier II
16 NC_000001.11 1 243480449 + SDCCAG8 NNNTGGGTGGGATGGATGAA NGG 2 0.025 Tier II
17 NC_000001.11 1 243480732 + SDCCAG8 NNNTGGGTGGGATGGATGAA NGG 2 0.025 Tier II
18 NC_000019.10 19 3153710 + GNA15 NNNTGGGTGGGATGGATGAA NGG 2 0.025 Tier II
19 NC_000001.11 1 243480648 + SDCCAG8 NNNTGGGTGTGATGGATGGA NGG 2 0.0188 Tier II
20 NC_000011.10 11 43755404 + HSD17B12 NNNTGGGTGTGATGCATGCA NGA 2 0.0144 Tier II
21 NC_000006.12 6 152130160 + SYNE1 NNNTGGGTGTGCTGGATGAA NGG 2 0.0132 Tier II
22 NC_000004.12 4 82745725 - SCD5 NNNTGGGTGTGATTCATGAA NTG 2 0.0104 Tier II
23 NC_000002.12 2 239159510 - HDAC4 NNNTGGGTGTGAGGCATGAA NTG 2 0.0102 Tier II
24 NC_000001.11 1 18667732 - PAX7 NNNTGGGTGTGATGCAGGAA NGT 2 0.0019 Tier II
25 NC_000001.11 1 58410399 - DAB1 NNNTGGGTGGGATGCTTGAA NGG 2 0.0 Tier II
26 NC_000002.12 2 100294037 + LONRF2 NNNTGGGTGTGATACTTGAA NGG 2 0.0 Tier II
27 NC_000013.11 13 73572905 - LINC00392 NNNTGGATGTGATGCAAGAA NGG 2 0.5333 Tier III
28 NC_000013.11 13 73572905 - LINC00393 NNNTGGATGTGATGCAAGAA NGG 2 0.5333 Tier III
29 NC_000004.12 4 169304721 + LOC105377529 NNNTGGGTGAGTTGCATGAA NGG 2 0.2857 Tier III
30 NC_000003.12 3 18163770 + BALR6 NNNTGAGTGTGATGCATGAC NGG 2 0.2273 Tier III
31 NC_000009.12 9 38363633 - LOC105376040 NNNTTGGTGTGGTGCATGAA NGG 2 0.2167 Tier III
32 NC_000009.12 9 38363633 - LOC107987065 NNNTTGGTGTGGTGCATGAA NGG 2 0.2167 Tier III
33 NC_000003.12 3 139515625 + COPB2-DT NNNTGTGTGTGATGAATGAA NGG 2 0.1481 Tier III
34 NC_000016.10 16 82965863 - LOC101928417 NNNTGGGTGTGATGTATGTA NGG 2 0.0359 Tier III
35 NC_000019.10 19 3153710 + GNA15-DT NNNTGGGTGGGATGGATGAA NGG 2 0.025 Tier III
36 NC_000014.9 14 105907076 + IGH NNNTGGGTGTGATGTATGGA NGG 2 0.025 Tier III
37 NC_000004.12 4 14508660 - LINC00504 NNNTGGGTGTGAAGCATGAA NGC 2 0.0154 Tier III
38 NC_000002.12 2 84213344 + LOC107985905 NNNTGGGTGTGATGCCAGAA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 136174095 + Pak7 NNNTGGGTGTGATGCATGAT NGG 1 0.6 Tier I
2 NC_000070.6 4 139423321 + Ubr4 NNNCAGGTGTGATGCATGAA NGG 2 0.5608 Tier II
3 NC_000081.6 15 59507029 - Nsmce2 NNNTGGGTCTGATGCATGAA NGG 1 0.5385 Tier II
4 NC_000073.6 7 115672036 - Sox6 NNNTGGGTGGGATGCATGAA NGG 1 0.5 Tier II
5 NC_000067.6 1 64696025 + Ccnyl1 NNNAGGGTGTAATGCATGAA NGG 2 0.4762 Tier II
6 NC_000073.6 7 142369531 - Ifitm10 NNNTGAGTGTGTTGCATGAA NGG 2 0.3333 Tier II
7 NC_000084.6 18 10175288 + Rock1 NNNTGGTTGGGATGCATGAA NGG 2 0.2857 Tier II
8 NC_000084.6 18 36368609 + Cystm1 NNNTGGATGTGATGCATGCA NGG 2 0.2069 Tier II
9 NC_000070.6 4 6735938 - Tox NNNTGGGTTTTATGCATGAA NGG 2 0.2051 Tier II
10 NC_000077.6 11 61738339 - Prpsap2 NNNTGGGTGTGATGCAAGGA NGG 2 0.2 Tier II
11 NC_000083.6 17 70381289 - Dlgap1 NNNTGGCTGTGATGCATGAC NGG 2 0.1563 Tier II
12 NC_000074.6 8 81878646 + Inpp4b NNNTGGGTGTGAAGCATGCA NGG 2 0.1432 Tier II
13 NC_000080.6 14 117591865 + Gpc6 NNNTGGGTCTGATGAATGAA NGG 2 0.1197 Tier II
14 NC_000080.6 14 28373051 - Erc2 NNNTGGGTGTGTGGCATGAA NGG 2 0.087 Tier II
15 NC_000067.6 1 12765327 - Sulf1 NNNTGGGTGTCATGCAGGAA NGG 2 0.0504 Tier II
16 NC_000071.6 5 125135608 - Ncor2 NNNTGTGTGTGATGCATGAA NGA 2 0.0463 Tier II
17 NC_000068.7 2 4351150 - Frmd4a NNNTGGGTGTGATAGATGAA NGG 2 0.0375 Tier II
18 NC_000075.6 9 18521411 + Muc16 NNNTGTGTGTGATGGATGAA NGG 2 0.0333 Tier II
19 NC_000072.6 6 127504205 - Parp11 NNNTGGGTGAGATGCATGAA NGC 2 0.019 Tier II
20 NC_000072.6 6 11919586 - Phf14 NNNTGGGTGTGAGGCATGAA NGA 2 0.0181 Tier II
21 NC_000077.6 11 9309765 - Abca13 NNNTGGGTGTGATGGATGAA NAG 2 0.013 Tier II
22 NC_000068.7 2 70739087 - Tlk1 NNNTGGGTGTGATGGATGAA NGC 2 0.0011 Tier II
23 NC_000081.6 15 84712010 + Gm41374 NNNTGGTTGGGATGCATGAA NGG 2 0.2857 Tier III
24 NC_000067.6 1 33147630 - Gm39616 NNNTAGGTGTGATGCATGCA NGG 2 0.1793 Tier III
25 NC_000067.6 1 169140644 - Gm39697 NNNTGTGTGTGATGCATGAA NAG 2 0.1728 Tier III
26 NC_000080.6 14 28373051 - Gm52097 NNNTGGGTGTGTGGCATGAA NGG 2 0.087 Tier III
27 NC_000082.6 16 95766086 - Gm32130 NNNTGGGTGTGTTGGATGAA NGG 2 0.0167 Tier III
28 NC_000068.7 2 166293045 - Gm11468 NNNTGGGTGTGAGGGATGAA NGG 2 0.013 Tier III
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Other clones with same target sequence:

(none)