Construct: sgRNA BRDN0001147132
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTAGGAGAGATGGAGACCAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- PRKAG3 (53632)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000002.12 | 2 | 218827598 | + | PRKAG3 | NNNGGAGAGATGGAGACCAG | NGG | 0 | 1.0 | Tier I |
| 2 | NC_000003.12 | 3 | 142519747 | - | ATR | NNNAGAGAGATGGAGACCAA | NGG | 2 | 0.8438 | Tier I |
| 3 | NC_000007.14 | 7 | 105581456 | - | EFCAB10 | NNNGGAGCGATGGAGACCAG | NAG | 2 | 0.1111 | Tier I |
| 4 | NC_000002.12 | 2 | 236412489 | - | IQCA1 | NNNGGAGAGATAAAGACCAG | NGG | 2 | 0.8615 | Tier II |
| 5 | NC_000005.10 | 5 | 93788330 | + | ARB2A | NNNGGTGAGATGAAGACCAG | NGG | 2 | 0.6593 | Tier II |
| 6 | NC_000012.12 | 12 | 47865267 | - | VDR | NNNGGAGAAATGGAGACCAG | NGG | 1 | 0.6429 | Tier II |
| 7 | NC_000005.10 | 5 | 137965463 | - | FAM13B | NNNGAAGGGATGGAGACCAG | NGG | 2 | 0.6356 | Tier II |
| 8 | NC_000001.11 | 1 | 190105635 | - | BRINP3 | NNNGGAGAAATAGAGACCAG | NGG | 2 | 0.6 | Tier II |
| 9 | NC_000001.11 | 1 | 24342830 | + | GRHL3 | NNNGGGGAGAAGGAGACCAG | NGG | 2 | 0.5357 | Tier II |
| 10 | NC_000007.14 | 7 | 87369057 | - | ABCB4 | NNNGGGGAGAAGGAGACCAG | NGG | 2 | 0.5357 | Tier II |
| 11 | NC_000007.14 | 7 | 87369057 | - | CROT | NNNGGGGAGAAGGAGACCAG | NGG | 2 | 0.5357 | Tier II |
| 12 | NC_000022.11 | 22 | 37498955 | + | CARD10 | NNNGGAGAGGTGGAAACCAG | NGG | 2 | 0.5229 | Tier II |
| 13 | NC_000002.12 | 2 | 79513484 | - | CTNNA2 | NNNGAAGAGATGGAGACAAG | NGG | 2 | 0.4667 | Tier II |
| 14 | NC_000009.12 | 9 | 15887910 | - | CCDC171 | NNNGGAAAGATGGAGAACAG | NGG | 2 | 0.4667 | Tier II |
| 15 | NC_000011.10 | 11 | 30531391 | + | MPPED2 | NNNGGAGAGATGGAAAACAG | NGG | 2 | 0.4392 | Tier II |
| 16 | NC_000016.10 | 16 | 69925584 | - | WWP2 | NNNGGAGAGATGGAAAACAG | NGG | 2 | 0.4392 | Tier II |
| 17 | NC_000017.11 | 17 | 82088059 | + | FASN | NNNGGAGAGTTGGAGATCAG | NGG | 2 | 0.4118 | Tier II |
| 18 | NC_000005.10 | 5 | 138078227 | + | WNT8A | NNNGGAGGGGTGGAGACCAG | NGG | 2 | 0.4074 | Tier II |
| 19 | NC_000010.11 | 10 | 11206581 | - | CELF2 | NNNGGTGAGGTGGAGACCAG | NGG | 2 | 0.3968 | Tier II |
| 20 | NC_000023.11 | X | 103254326 | - | TCEAL8 | NNNGGAGTGTTGGAGACCAG | NGG | 2 | 0.3782 | Tier II |
| 21 | NC_000008.11 | 8 | 4590270 | + | CSMD1 | NNNGGAGAGATGGTGACCAT | NGG | 2 | 0.3733 | Tier II |
| 22 | NC_000005.10 | 5 | 133790066 | - | FSTL4 | NNNGGAGAGGCGGAGACCAG | NGG | 2 | 0.3704 | Tier II |
| 23 | NC_000018.10 | 18 | 54757092 | + | RAB27B | NNNTGAGAGATGGAGACCAG | NGG | 1 | 0.3636 | Tier II |
| 24 | NC_000020.11 | 20 | 44394331 | - | HNF4A | NNNGGGGAGATGGAGATCAG | NGG | 2 | 0.3333 | Tier II |
| 25 | NC_000002.12 | 2 | 162590720 | - | KCNH7 | NNNGGAGAAATGGAGATCAG | NGG | 2 | 0.3 | Tier II |
| 26 | NC_000019.10 | 19 | 51322526 | - | IGLON5 | NNNGGAGAGGTGGAGACCTG | NGG | 2 | 0.2991 | Tier II |
| 27 | NC_000009.12 | 9 | 110967883 | - | LPAR1 | NNNGGAGAGATGCAGACCAT | NGG | 2 | 0.2947 | Tier II |
| 28 | NC_000013.11 | 13 | 33884853 | + | RFC3 | NNNGGACAGATGCAGACCAG | NGG | 2 | 0.2895 | Tier II |
| 29 | NC_000009.12 | 9 | 123646321 | + | DENND1A | NNNGGAGAGAATGAGACCAG | NGG | 2 | 0.2885 | Tier II |
| 30 | NC_000020.11 | 20 | 34571149 | - | PIGU | NNNGGAGGGATTGAGACCAG | NGG | 2 | 0.2821 | Tier II |
| 31 | NC_000008.11 | 8 | 21793304 | + | GFRA2 | NNNTGAGGGATGGAGACCAG | NGG | 2 | 0.2667 | Tier II |
| 32 | NC_000011.10 | 11 | 19594700 | + | NAV2 | NNNGGAGAGGTGGAGAACAG | NGG | 2 | 0.2593 | Tier II |
| 33 | NC_000003.12 | 3 | 60400278 | + | FHIT | NNNGGAGACATGGAGAACAG | NGG | 2 | 0.2513 | Tier II |
| 34 | NC_000012.12 | 12 | 26063983 | - | RASSF8 | NNNGGAGACATGGGGACCAG | NGG | 2 | 0.2513 | Tier II |
| 35 | NC_000001.11 | 1 | 215590439 | - | KCTD3 | NNNGGAGAGATGGAGAAAAG | NGG | 2 | 0.2513 | Tier II |
| 36 | NC_000008.11 | 8 | 84289040 | + | RALYL | NNNGGTGAGCTGGAGACCAG | NGG | 2 | 0.2381 | Tier II |
| 37 | NC_000001.11 | 1 | 158071066 | - | KIRREL1 | NNNAGAGAGATGGAGACCAG | NAG | 2 | 0.2333 | Tier II |
| 38 | NC_000011.10 | 11 | 100127169 | - | CNTN5 | NNNGGATAGATTGAGACCAG | NGG | 2 | 0.2198 | Tier II |
| 39 | NC_000008.11 | 8 | 11707453 | + | GATA4 | NNNGGAGAGATGGGGAACAG | NGG | 2 | 0.2178 | Tier II |
| 40 | NC_000023.11 | X | 3015717 | + | ARSH | NNNGGAGACAGGGAGACCAG | NGG | 2 | 0.2154 | Tier II |
| 41 | NC_000009.12 | 9 | 136747982 | - | LCN6 | NNNGGAGAGCTGGAGACTAG | NGG | 2 | 0.2143 | Tier II |
| 42 | NC_000002.12 | 2 | 49014477 | - | FSHR | NNNGGAGAGAGGGAGAACAG | NGG | 2 | 0.1867 | Tier II |
| 43 | NC_000014.9 | 14 | 46893382 | + | MDGA2 | NNNGGAGAGATGGAGGCCAA | NGG | 2 | 0.1803 | Tier II |
| 44 | NC_000008.11 | 8 | 143595884 | + | EEF1D | NNNGGAGAGCTGGAGACCTG | NGG | 2 | 0.1795 | Tier II |
| 45 | NC_000023.11 | X | 97373961 | + | DIAPH2 | NNNGGAGCGAGGGAGACCAG | NGG | 2 | 0.1714 | Tier II |
| 46 | NC_000017.11 | 17 | 39688590 | - | ERBB2 | NNNGGAGTGAGGGAGACCAG | NGG | 2 | 0.1714 | Tier II |
| 47 | NC_000011.10 | 11 | 130462834 | + | ADAMTS15 | NNNGGAGGGATGGAGACCCG | NGG | 2 | 0.1517 | Tier II |
| 48 | NC_000014.9 | 14 | 103109175 | - | EXOC3L4 | NNNGGAGAGAGGGAGACCGG | NGG | 2 | 0.15 | Tier II |
| 49 | NC_000008.11 | 8 | 139981298 | - | TRAPPC9 | NNNGGGGAGATGGAGACCCG | NGG | 2 | 0.1478 | Tier II |
| 50 | NC_000019.10 | 19 | 7466989 | - | ARHGEF18 | NNNGGGGAGATGGAGACCCG | NGG | 2 | 0.1478 | Tier II |
| 51 | NC_000023.11 | X | 10121624 | + | WWC3 | NNNGGAGAGATGGTCACCAG | NGG | 2 | 0.1455 | Tier II |
| 52 | NC_000007.14 | 7 | 3539196 | + | SDK1 | NNNGGGGAGATGGAGGCCAG | NGG | 2 | 0.1374 | Tier II |
| 53 | NC_000014.9 | 14 | 104693767 | - | INF2 | NNNGGGGAGATGGAGGCCAG | NGG | 2 | 0.1374 | Tier II |
| 54 | NC_000016.10 | 16 | 10895947 | + | CIITA | NNNGGGGAGATGGAGGCCAG | NGG | 2 | 0.1374 | Tier II |
| 55 | NC_000017.11 | 17 | 57280437 | + | MSI2 | NNNGTAGTGATGGAGACCAG | NGG | 2 | 0.1286 | Tier II |
| 56 | NC_000022.11 | 22 | 33922065 | - | LARGE1 | NNNGGAGAGGTGGAGACCCG | NGG | 2 | 0.1149 | Tier II |
| 57 | NC_000006.12 | 6 | 107625286 | + | SOBP | NNNGCAGAGATGGATACCAG | NGG | 2 | 0.1122 | Tier II |
| 58 | NC_000019.10 | 19 | 49973475 | - | SIGLEC16 | NNNGGAGAGATGGAGGCAAG | NGG | 2 | 0.1036 | Tier II |
| 59 | NC_000002.12 | 2 | 98167132 | + | VWA3B | NNNGGAGAGATGGTGGCCAG | NGG | 2 | 0.1026 | Tier II |
| 60 | NC_000001.11 | 1 | 53274524 | + | LRP8 | NNNGTAGAGATGTAGACCAG | NGG | 2 | 0.09 | Tier II |
| 61 | NC_000001.11 | 1 | 41531248 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 62 | NC_000001.11 | 1 | 41531280 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 63 | NC_000001.11 | 1 | 41531312 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 64 | NC_000001.11 | 1 | 41531424 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 65 | NC_000001.11 | 1 | 41531536 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 66 | NC_000001.11 | 1 | 41531600 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 67 | NC_000001.11 | 1 | 41531662 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 68 | NC_000001.11 | 1 | 41531758 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 69 | NC_000001.11 | 1 | 41531822 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 70 | NC_000001.11 | 1 | 41531884 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 71 | NC_000001.11 | 1 | 41531996 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 72 | NC_000001.11 | 1 | 41532060 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 73 | NC_000001.11 | 1 | 41532122 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 74 | NC_000001.11 | 1 | 41532232 | + | HIVEP3 | NNNGGAGAGATGGAGGACAG | NGG | 2 | 0.0897 | Tier II |
| 75 | NC_000011.10 | 11 | 34266864 | + | ABTB2 | NNNGGAGAGATGGGGGCCAG | NGG | 2 | 0.0897 | Tier II |
| 76 | NC_000005.10 | 5 | 22743187 | - | CDH12 | NNNGGAGAGCTGGAGACCAG | NAG | 2 | 0.0864 | Tier II |
| 77 | NC_000002.12 | 2 | 65057002 | + | CEP68 | NNNGGAAAGATGGAGACCAG | NGA | 2 | 0.0694 | Tier II |
| 78 | NC_000003.12 | 3 | 79185230 | - | ROBO1 | NNNGGAGAGATAGAGACCAG | NGA | 2 | 0.0648 | Tier II |
| 79 | NC_000002.12 | 2 | 66476887 | + | MEIS1 | NNNGGAGAGATGGAGAAGAG | NGG | 2 | 0.0622 | Tier II |
| 80 | NC_000016.10 | 16 | 67002482 | + | CES4A | NNNGGAAAGATGGAGAGCAG | NGG | 2 | 0.0588 | Tier II |
| 81 | NC_000010.11 | 10 | 10777015 | - | CELF2 | NNNGGAGAGATGGAGACCAT | NGA | 2 | 0.0486 | Tier II |
| 82 | NC_000023.11 | X | 46615413 | + | SLC9A7 | NNNGGAGAGATGCAGACCAG | NCG | 2 | 0.0451 | Tier II |
| 83 | NC_000001.11 | 1 | 111859014 | - | KCND3 | NNNGGAGAAATGGAGACCAG | NGA | 2 | 0.0446 | Tier II |
| 84 | NC_000007.14 | 7 | 133581934 | + | EXOC4 | NNNGGAGAGAAGGAGAGCAG | NGG | 2 | 0.0441 | Tier II |
| 85 | NC_000001.11 | 1 | 90712128 | + | BARHL2 | NNNGGGGAGATGGAGAGCAG | NGG | 2 | 0.042 | Tier II |
| 86 | NC_000006.12 | 6 | 30158210 | + | TRIM10 | NNNGGAGAGGTGGAGACCAG | NGA | 2 | 0.0386 | Tier II |
| 87 | NC_000012.12 | 12 | 110126731 | + | IFT81 | NNNGGAGAGGTGGAGACCAG | NGA | 2 | 0.0386 | Tier II |
| 88 | NC_000007.14 | 7 | 110668387 | - | IMMP2L | NNNGAAGAGATGGAGACCAG | NTG | 2 | 0.0338 | Tier II |
| 89 | NC_000003.12 | 3 | 192800064 | - | MB21D2 | NNNGGAGAGATGGAGAACAG | NGA | 2 | 0.0324 | Tier II |
| 90 | NC_000019.10 | 19 | 48735570 | + | RASIP1 | NNNGGAGAGATGGAGAACAG | NGA | 2 | 0.0324 | Tier II |
| 91 | NC_000001.11 | 1 | 171063998 | + | MROH9 | NNNGGAGAGATGGAGAGAAG | NGG | 2 | 0.0317 | Tier II |
| 92 | NC_000012.12 | 12 | 112376624 | - | HECTD4 | NNNGGAGAGATGGGGAGCAG | NGG | 2 | 0.0275 | Tier II |
| 93 | NC_000017.11 | 17 | 82410025 | - | OGFOD3 | NNNGGAGAGATGGGGAGCAG | NGG | 2 | 0.0275 | Tier II |
| 94 | NC_000009.12 | 9 | 122383357 | + | PTGS1 | NNNGTAGAGATGGAGAGCAG | NGG | 2 | 0.0176 | Tier II |
| 95 | NC_000018.10 | 18 | 11149392 | + | PIEZO2 | NNNGGAGAAATGGAGACCAG | NGC | 2 | 0.0143 | Tier II |
| 96 | NC_000001.11 | 1 | 173522462 | + | SLC9C2 | NNNGGAGGGATGGAGACCAG | NGT | 2 | 0.0118 | Tier II |
| 97 | NC_000019.10 | 19 | 46068135 | - | IGFL4 | NNNGGAGAGATGGAGAACAG | NGC | 2 | 0.0104 | Tier II |
| 98 | NC_000003.12 | 3 | 43393643 | - | ANO10 | NNNGGAGACATGGAGACCAG | NGT | 2 | 0.0087 | Tier II |
| 99 | NC_000019.10 | 19 | 51084068 | + | KLK14 | NNNGGAGAGATGGAGAGGAG | NGG | 2 | 0.0078 | Tier II |
| 100 | NC_000020.11 | 20 | 43049085 | - | PTPRT | NNNGGAGAGATGGAGAACAG | NGT | 2 | 0.0075 | Tier II |
| 101 | NC_000007.14 | 7 | 39179702 | + | POU6F2 | NNNGGAGAGATGGAGAGCAG | NTG | 2 | 0.0023 | Tier II |
| 102 | NC_000007.14 | 7 | 70620757 | - | AUTS2 | NNNGGAGAAATGGAGCCCAG | NGG | 2 | 0.0 | Tier II |
| 103 | NC_000001.11 | 1 | 3697337 | - | TP73 | NNNGGAGAGACGGAGCCCAG | NGG | 2 | 0.0 | Tier II |
| 104 | NC_000007.14 | 7 | 100970119 | + | MUC12 | NNNGGAGAGAGGGAGTCCAG | NGG | 2 | 0.0 | Tier II |
| 105 | NC_000002.12 | 2 | 85697882 | - | GNLY | NNNGGAGAGATGGAGACCAG | NTT | 2 | 0.0 | Tier II |
| 106 | NC_000005.10 | 5 | 54054138 | - | ARL15 | NNNGGAGAGATGGAGCACAG | NGG | 2 | 0.0 | Tier II |
| 107 | NC_000022.11 | 22 | 29789338 | + | ASCC2 | NNNGGAGAGATGGAGCCCAG | NAG | 2 | 0.0 | Tier II |
| 108 | NC_000019.10 | 19 | 44498338 | - | ZNF180 | NNNGGAGAGATGGAGTGCAG | NGG | 2 | 0.0 | Tier II |
| 109 | NC_000020.11 | 20 | 61900737 | + | CDH4 | NNNGGAGAGATGGGGCCCAG | NGG | 2 | 0.0 | Tier II |
| 110 | NC_000011.10 | 11 | 125921459 | + | DDX25 | NNNGGAGAGCTGGAGTCCAG | NGG | 2 | 0.0 | Tier II |
| 111 | NC_000002.12 | 2 | 98207343 | - | VWA3B | NNNTGAGAGATGGAGTCCAG | NGG | 2 | 0.0 | Tier II |
| 112 | NC_000017.11 | 17 | 36940997 | + | LHX1 | NNNTGAGAGATGGAGTCCAG | NGG | 2 | 0.0 | Tier II |
| 113 | NC_000022.11 | 22 | 39293456 | - | LOC124905119 | NNNGGAGAGATGGAAACCAG | NGG | 1 | 0.9412 | Tier III |
| 114 | NC_000002.12 | 2 | 74748909 | - | LOC102724497 | NNNGGAGAGATGGAGACCAA | NGG | 1 | 0.9375 | Tier III |
| 115 | NC_000013.11 | 13 | 22977093 | + | LOC105370111 | NNNGGGAAGATGGAGACCAG | NGG | 2 | 0.7143 | Tier III |
| 116 | NC_000018.10 | 18 | 21915500 | + | LOC105372016 | NNNGGAGAGACGGAAACCAG | NGG | 2 | 0.6275 | Tier III |
| 117 | NC_000011.10 | 11 | 116440173 | + | LOC107987166 | NNNGAAGAGATGGAGACTAG | NGG | 2 | 0.5571 | Tier III |
| 118 | NC_000018.10 | 18 | 76783716 | + | LOC124904327 | NNNGGAGAGGTGAAGACCAG | NGG | 2 | 0.5128 | Tier III |
| 119 | NC_000010.11 | 10 | 11206581 | - | LOC124902375 | NNNGGTGAGGTGGAGACCAG | NGG | 2 | 0.3968 | Tier III |
| 120 | NC_000005.10 | 5 | 157576336 | + | LOC124901121 | NNNGGAGTGATGAAGACCAG | NGG | 2 | 0.3956 | Tier III |
| 121 | NC_000022.11 | 22 | 38677758 | - | TOMM22-DT | NNNGGAGAGAGGGAAACCAG | NGG | 2 | 0.3765 | Tier III |
| 122 | NC_000020.11 | 20 | 44394331 | - | HNF4A-AS1 | NNNGGGGAGATGGAGATCAG | NGG | 2 | 0.3333 | Tier III |
| 123 | NC_000007.14 | 7 | 156476162 | + | RNF32-DT | NNNGAAGAGATGGAGACCAG | NAG | 2 | 0.2247 | Tier III |
| 124 | NC_000009.12 | 9 | 136747982 | - | LOC100128593 | NNNGGAGAGCTGGAGACTAG | NGG | 2 | 0.2143 | Tier III |
| 125 | NC_000005.10 | 5 | 4846926 | - | LOC107986400 | NNNGGAGAGATTTAGACCAG | NGG | 2 | 0.1154 | Tier III |
| 126 | NC_000022.11 | 22 | 33922065 | - | LARGE1-AS1 | NNNGGAGAGGTGGAGACCCG | NGG | 2 | 0.1149 | Tier III |
| 127 | NC_000020.11 | 20 | 50253118 | + | LOC105372656 | NNNGGAGACATGGAGGCCAG | NGG | 2 | 0.1036 | Tier III |
| 128 | NC_000018.10 | 18 | 55906277 | + | LINC01416 | NNNGTAGAGATGGAGACCAG | NAG | 2 | 0.0778 | Tier III |
| 129 | NC_000016.10 | 16 | 84934227 | - | LINC02176 | NNNGGAGAGAGGGAGGCCAG | NGG | 2 | 0.0769 | Tier III |
| 130 | NC_000002.12 | 2 | 241892405 | - | LINC01237 | NNNGGAGAGAAGGAGACCAG | NGA | 2 | 0.0521 | Tier III |
| 131 | NC_000001.11 | 1 | 230879924 | - | LOC124904547 | NNNGGAGAGATGGAGGCCAG | NAG | 2 | 0.0499 | Tier III |
| 132 | NC_000018.10 | 18 | 2363908 | - | LOC105371961 | NNNGGAGAGATGGAGGCCAG | NAG | 2 | 0.0499 | Tier III |
| 133 | NC_000023.11 | X | 46615413 | + | LOC124905182 | NNNGGAGAGATGCAGACCAG | NCG | 2 | 0.0451 | Tier III |
| 134 | NC_000011.10 | 11 | 102684561 | + | MMP20-AS1 | NNNGGAGAGAGGGAGAGCAG | NGG | 2 | 0.0235 | Tier III |
| 135 | NC_000017.11 | 17 | 5863150 | - | LOC339166 | NNNGGAGAGAGGGAGAGCAG | NGG | 2 | 0.0235 | Tier III |
| 136 | NC_000001.11 | 1 | 213914950 | - | PROX1-AS1 | NNNAGAGAGATGGAGACCAG | NGC | 2 | 0.02 | Tier III |
| 137 | NC_000023.11 | X | 128504760 | + | LOC107985698 | NNNGGAGAGATAGAGACCAG | NGT | 2 | 0.0151 | Tier III |
| 138 | NC_000010.11 | 10 | 42756077 | - | LOC105378269 | NNNGGAGAGATGGAGACAAG | NGT | 2 | 0.0087 | Tier III |
| 139 | NC_000001.11 | 1 | 243982982 | + | LINC02774 | NNNGGAGAGAAGGAGTCCAG | NGG | 2 | 0.0 | Tier III |
| 140 | NC_000022.11 | 22 | 44624107 | + | LINC00229 | NNNGGAGAGAGGGAGCCCAG | NGG | 2 | 0.0 | Tier III |
| 141 | NC_000022.11 | 22 | 44624107 | + | LOC105373060 | NNNGGAGAGAGGGAGCCCAG | NGG | 2 | 0.0 | Tier III |
| 142 | NC_000016.10 | 16 | 56986256 | + | LOC124903696 | NNNGGAGATATGGAGTCCAG | NGG | 2 | 0.0 | Tier III |
| 143 | NC_000002.12 | 2 | 98207343 | - | ATP5F1BP1 | NNNTGAGAGATGGAGTCCAG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | NC_000067.6 | 1 | 74745340 | + | Prkag3 | NNNGGAGAGATGGAGACTAG | NGG | 1 | 0.6429 | Tier I |
| 2 | NC_000081.6 | 15 | 98815206 | + | Prkag1 | NNNGGAGAGATGCAGACAAG | NGG | 2 | 0.2267 | Tier I |
| 3 | NC_000073.6 | 7 | 125843242 | + | D430042O09Rik | NNNGGAGAGGTGGAGACCAG | NGA | 2 | 0.0386 | Tier I |
| 4 | NC_000067.6 | 1 | 37665042 | - | Cracdl | NNNAGAGAGATGGAAACCAG | NGG | 2 | 0.8471 | Tier II |
| 5 | NC_000067.6 | 1 | 132332754 | - | Nuak2 | NNNGCAGAGATGGAAACCAG | NGG | 2 | 0.7395 | Tier II |
| 6 | NC_000079.6 | 13 | 8711868 | - | Adarb2 | NNNGGGAAGATGGAGACCAG | NGG | 2 | 0.7143 | Tier II |
| 7 | NC_000081.6 | 15 | 79395955 | + | Tmem184b | NNNACAGAGATGGAGACCAG | NGG | 2 | 0.7071 | Tier II |
| 8 | NC_000072.6 | 6 | 65411224 | + | Qrfprl | NNNGGAGAGATAGAGACTAG | NGG | 2 | 0.6 | Tier II |
| 9 | NC_000076.6 | 10 | 88776559 | + | Utp20 | NNNAGAGAAATGGAGACCAG | NGG | 2 | 0.5786 | Tier II |
| 10 | NC_000076.6 | 10 | 18193545 | - | Ect2l | NNNGGAGAGGTGGAGACCAG | NGG | 1 | 0.5556 | Tier II |
| 11 | NC_000073.6 | 7 | 97251427 | - | Gab2 | NNNGCAGAAATGGAGACCAG | NGG | 2 | 0.5051 | Tier II |
| 12 | NC_000082.6 | 16 | 87555379 | - | Map3k7cl | NNNGGAGAGATAGAGACAAG | NGG | 2 | 0.5026 | Tier II |
| 13 | NC_000074.6 | 8 | 25572320 | - | Fgfr1 | NNNAGAGAGGTGGAGACCAG | NGG | 2 | 0.5 | Tier II |
| 14 | NC_000082.6 | 16 | 9681765 | + | Grin2a | NNNGGAGAGATGATGACCAG | NGG | 2 | 0.4923 | Tier II |
| 15 | NC_000070.6 | 4 | 127225850 | - | Dlgap3 | NNNAGAGAGATGGAGACAAG | NGG | 2 | 0.4846 | Tier II |
| 16 | NC_000069.6 | 3 | 122139566 | - | Abca4 | NNNGGAGAGTTGGAGACCTG | NGG | 2 | 0.4751 | Tier II |
| 17 | NC_000079.6 | 13 | 18169319 | - | Pou6f2 | NNNGAAGAGATGGAGACCTG | NGG | 2 | 0.4667 | Tier II |
| 18 | NC_000081.6 | 15 | 78023958 | - | Cacng2 | NNNGGAAAGATGGAGACCAC | NGG | 2 | 0.4286 | Tier II |
| 19 | NC_000071.6 | 5 | 101925597 | - | Wdfy3 | NNNGGAATGATGGAGACCAG | NGG | 2 | 0.4286 | Tier II |
| 20 | NC_000085.6 | 19 | 16157360 | + | Gnaq | NNNGGAGAGAAGGAGACAAG | NGG | 2 | 0.4038 | Tier II |
| 21 | NC_000071.6 | 5 | 134229220 | - | Ncf1 | NNNGGAGAGAGGGAGACCAG | NGG | 1 | 0.4 | Tier II |
| 22 | NC_000081.6 | 15 | 79816565 | - | Npcd | NNNGGGGAGGTGGAGACCAG | NGG | 2 | 0.3968 | Tier II |
| 23 | NC_000084.6 | 18 | 20958246 | - | Rnf125 | NNNGGGGAGGTGGAGACCAG | NGG | 2 | 0.3968 | Tier II |
| 24 | NC_000085.6 | 19 | 42175426 | + | Zfyve27 | NNNGGGGAGGTGGAGACCAG | NGG | 2 | 0.3968 | Tier II |
| 25 | NC_000073.6 | 7 | 142459371 | - | Lsp1 | NNNGGAGAGGTGGAGACCAT | NGG | 2 | 0.3889 | Tier II |
| 26 | NC_000083.6 | 17 | 43877651 | - | Rcan2 | NNNAGAGTGATGGAGACCAG | NGG | 2 | 0.3857 | Tier II |
| 27 | NC_000071.6 | 5 | 139968419 | + | Elfn1 | NNNAGAGAGATGCAGACCAG | NGG | 2 | 0.3789 | Tier II |
| 28 | NC_000074.6 | 8 | 46500428 | - | Acsl1 | NNNGGAGAGAGGGAAACCAG | NGG | 2 | 0.3765 | Tier II |
| 29 | NC_000071.6 | 5 | 81031830 | - | Adgrl3 | NNNGGAGAGAGAGAGACCAG | NGG | 2 | 0.3733 | Tier II |
| 30 | NC_000079.6 | 13 | 63075658 | + | Aopep | NNNGGAGAGATTAAGACCAG | NGG | 2 | 0.355 | Tier II |
| 31 | NC_000068.7 | 2 | 145790665 | + | Rin2 | NNNGGAGAGAAGGAGAACAG | NGG | 2 | 0.35 | Tier II |
| 32 | NC_000076.6 | 10 | 14531760 | - | Adgrg6 | NNNGGACAGATGGAGATCAG | NGG | 2 | 0.3208 | Tier II |
| 33 | NC_000072.6 | 6 | 38949819 | - | Tbxas1 | NNNGGAGAGCTGAAGACCAG | NGG | 2 | 0.3077 | Tier II |
| 34 | NC_000074.6 | 8 | 22084215 | - | Nek5 | NNNGTAGAGATGGAGACCAG | NGG | 1 | 0.3 | Tier II |
| 35 | NC_000070.6 | 4 | 140203467 | - | Igsf21 | NNNGGAGAGGTGGAGACAAG | NGG | 2 | 0.2991 | Tier II |
| 36 | NC_000070.6 | 4 | 108259205 | + | Zyg11b | NNNGGAGATGTGGAGACCAG | NGG | 2 | 0.2963 | Tier II |
| 37 | NC_000084.6 | 18 | 5102769 | - | Svil | NNNGGAGAGATGGAGACATG | NGG | 2 | 0.2899 | Tier II |
| 38 | NC_000068.7 | 2 | 31459027 | - | Hmcn2 | NNNGGGGAGAGGGAGACCAG | NGG | 2 | 0.2857 | Tier II |
| 39 | NC_000068.7 | 2 | 172923281 | - | Bmp7 | NNNTGGGAGATGGAGACCAG | NGG | 2 | 0.2597 | Tier II |
| 40 | NC_000082.6 | 16 | 21853268 | + | Map3k13 | NNNGGAGACATGGAGAACAG | NGG | 2 | 0.2513 | Tier II |
| 41 | NC_000076.6 | 10 | 51729427 | + | Rfx6 | NNNGGAGAGATGGTGATCAG | NGG | 2 | 0.2489 | Tier II |
| 42 | NC_000076.6 | 10 | 80812894 | + | Jsrp1 | NNNGGAGAAATTGAGACCAG | NGG | 2 | 0.2473 | Tier II |
| 43 | NC_000068.7 | 2 | 153197301 | - | Tm9sf4 | NNNGGAGAGATGGAAACCAG | NAG | 2 | 0.244 | Tier II |
| 44 | NC_000071.6 | 5 | 88911168 | - | Slc4a4 | NNNGAAGAGATGGAGACCAG | NAG | 2 | 0.2247 | Tier II |
| 45 | NC_000067.6 | 1 | 188163716 | + | Esrrg | NNNGGAGAGGTGGAGACCGG | NGG | 2 | 0.2083 | Tier II |
| 46 | NC_000067.6 | 1 | 164309573 | + | Nme7 | NNNGGAGAGATGGAGACAGG | NGG | 2 | 0.2019 | Tier II |
| 47 | NC_000070.6 | 4 | 44986879 | - | Grhpr | NNNGGAGAGATGGAGACAGG | NGG | 2 | 0.2019 | Tier II |
| 48 | NC_000071.6 | 5 | 89794684 | - | Adamts3 | NNNGGAGAGATGGAGACAGG | NGG | 2 | 0.2019 | Tier II |
| 49 | NC_000081.6 | 15 | 39145436 | - | Dcaf13 | NNNGGAGAGATGGAGACAGG | NGG | 2 | 0.2019 | Tier II |
| 50 | NC_000078.6 | 12 | 98955795 | + | Ttc8 | NNNGGAGAGATGGAGAACAC | NGG | 2 | 0.2 | Tier II |
| 51 | NC_000068.7 | 2 | 126750418 | - | Usp8 | NNNGGAGAGATGCAGACCAC | NGG | 2 | 0.1805 | Tier II |
| 52 | NC_000080.6 | 14 | 64999622 | + | Ints9 | NNNTGAGAGATGGGGACCAG | NGG | 2 | 0.1697 | Tier II |
| 53 | NC_000072.6 | 6 | 97359136 | + | Frmd4b | NNNTGAGAGATGGAGACCAC | NGG | 2 | 0.1558 | Tier II |
| 54 | NC_000070.6 | 4 | 57037081 | + | Epb41l4b | NNNGGAGAGATGGACACAAG | NGG | 2 | 0.1469 | Tier II |
| 55 | NC_000074.6 | 8 | 77047332 | - | Nr3c2 | NNNGGAGAGAAGGAGGCCAG | NGG | 2 | 0.1442 | Tier II |
| 56 | NC_000070.6 | 4 | 109001232 | - | Nrd1 | NNNGGAGACATGGAGACCAG | NAG | 2 | 0.1396 | Tier II |
| 57 | NC_000077.6 | 11 | 76536328 | - | Abr | NNNGGAGATATGGAGACCAG | NAG | 2 | 0.1383 | Tier II |
| 58 | NC_000068.7 | 2 | 72078940 | + | Rapgef4 | NNNGGAGAGATGGAGGCCAT | NGG | 2 | 0.1346 | Tier II |
| 59 | NC_000085.6 | 19 | 9892590 | - | Incenp | NNNGGAGAGATAGATACCAG | NGG | 2 | 0.1333 | Tier II |
| 60 | NC_000074.6 | 8 | 84830606 | - | Dand5 | NNNGGAGAGATGGAGGCAAG | NGG | 2 | 0.1036 | Tier II |
| 61 | NC_000067.6 | 1 | 52139062 | - | Stat1 | NNNGGAGAGATGGAGGCCTG | NGG | 2 | 0.1036 | Tier II |
| 62 | NC_000068.7 | 2 | 155387829 | + | Trp53inp2 | NNNGGAGAGATGCAGGCCAG | NGG | 2 | 0.081 | Tier II |
| 63 | NC_000076.6 | 10 | 6834836 | - | Oprm1 | NNNGGAGAGGTGGATACCAG | NGG | 2 | 0.0794 | Tier II |
| 64 | NC_000071.6 | 5 | 125445418 | - | Bri3bp | NNNGTAGAGATGGAGACCAG | NAG | 2 | 0.0778 | Tier II |
| 65 | NC_000071.6 | 5 | 102389267 | - | Arhgap24 | NNNGGAGAGATGGAGAAGAG | NGG | 2 | 0.0622 | Tier II |
| 66 | NC_000067.6 | 1 | 100067334 | - | Cntnap5b | NNNGTAGAGATGGAGGCCAG | NGG | 2 | 0.0577 | Tier II |
| 67 | NC_000082.6 | 16 | 12081523 | + | Shisa9 | NNNGTAGAGATGGAGGCCAG | NGG | 2 | 0.0577 | Tier II |
| 68 | NC_000085.6 | 19 | 58994996 | + | Shtn1 | NNNGGAGAGAAGGAGAGCAG | NGG | 2 | 0.0441 | Tier II |
| 69 | NC_000070.6 | 4 | 98429854 | + | Patj | NNNGGAGGGATGGAGAGCAG | NGG | 2 | 0.0431 | Tier II |
| 70 | NC_000080.6 | 14 | 32641797 | - | Prrxl1 | NNNGGAGAGATGGAGAGTAG | NGG | 2 | 0.0378 | Tier II |
| 71 | NC_000085.6 | 19 | 27939952 | - | Rfx3 | NNNGGAGAGATGGGGACCAG | NGA | 2 | 0.0324 | Tier II |
| 72 | NC_000079.6 | 13 | 114850682 | - | Itga2 | NNNGGAGAGATGGAGAGAAG | NGG | 2 | 0.0317 | Tier II |
| 73 | NC_000078.6 | 12 | 98539354 | - | Kcnk10 | NNNGGAGAGATGGAGAGCTG | NGG | 2 | 0.0317 | Tier II |
| 74 | NC_000073.6 | 7 | 101352159 | - | Arap1 | NNNGGAGAGATGGATGCCAG | NGG | 2 | 0.0275 | Tier II |
| 75 | NC_000085.6 | 19 | 42596176 | + | Loxl4 | NNNTGAGAGATGGAGACCAG | NGA | 2 | 0.0253 | Tier II |
| 76 | NC_000070.6 | 4 | 70337577 | + | Cdk5rap2 | NNNGGAGAGAGGGAGAGCAG | NGG | 2 | 0.0235 | Tier II |
| 77 | NC_000080.6 | 14 | 9620163 | - | Fhit | NNNGGAGAGAGGGAGAGCAG | NGG | 2 | 0.0235 | Tier II |
| 78 | NC_000075.6 | 9 | 47840304 | + | Cadm1 | NNNTGAGAGATGGAGAGCAG | NGG | 2 | 0.0214 | Tier II |
| 79 | NC_000070.6 | 4 | 127192440 | + | Dlgap3 | NNNGGAGAGATGGAGACCAA | NGC | 2 | 0.0208 | Tier II |
| 80 | NC_000071.6 | 5 | 120174211 | + | Rbm19 | NNNGGAGAGCTGGAGAGCAG | NGG | 2 | 0.0196 | Tier II |
| 81 | NC_000073.6 | 7 | 19656284 | - | Clptm1 | NNNGGAGAGCTGGAGAGCAG | NGG | 2 | 0.0196 | Tier II |
| 82 | NC_000073.6 | 7 | 19725533 | - | Nectin2 | NNNGGAGAGATGGAGACCAC | NTG | 2 | 0.0167 | Tier II |
| 83 | NC_000074.6 | 8 | 18644600 | - | Mcph1 | NNNGGAGAGATGGAGACCAG | NGT | 1 | 0.0161 | Tier II |
| 84 | NC_000077.6 | 11 | 78157248 | - | Fam222b | NNNTGAGAGATGGAGACCAG | NTG | 2 | 0.0142 | Tier II |
| 85 | NC_000085.6 | 19 | 6115351 | - | Naaladl1 | NNNGGAGAGATGGCGAGCAG | NGG | 2 | 0.0126 | Tier II |
| 86 | NC_000076.6 | 10 | 50848862 | - | Ascc3 | NNNGGAGAGATGGAGACAAG | NGC | 2 | 0.012 | Tier II |
| 87 | NC_000068.7 | 2 | 37992757 | + | Dennd1a | NNNGGAGAGAGGGAGACCAG | NGC | 2 | 0.0089 | Tier II |
| 88 | NC_000084.6 | 18 | 75617205 | - | Ctif | NNNGGAGAGATGCAGACCAG | NGT | 2 | 0.0068 | Tier II |
| 89 | NC_000068.7 | 2 | 60547320 | + | Pla2r1 | NNNAGAGAGATGGAGTCCAG | NGG | 2 | 0.0 | Tier II |
| 90 | NC_000075.6 | 9 | 105660500 | - | Pik3r4 | NNNGGAAAGATGGAGTCCAG | NGG | 2 | 0.0 | Tier II |
| 91 | NC_000068.7 | 2 | 120036275 | - | Pla2g4b | NNNGGAGAGATGGAGCCCAG | NGG | 1 | 0.0 | Tier II |
| 92 | NC_000078.6 | 12 | 118259494 | - | Sp4 | NNNGGAGAGATGGAGTCCAG | NGG | 1 | 0.0 | Tier II |
| 93 | NC_000068.7 | 2 | 173119646 | + | Gm40011 | NNNGGAGAGATGGAGTCCTG | NGG | 2 | 0.0 | Tier II |
| 94 | NC_000086.7 | X | 100730277 | + | Gdpd2 | NNNGGAGGGATGGAGCCCAG | NGG | 2 | 0.0 | Tier II |
| 95 | NC_000067.6 | 1 | 37665042 | - | 4930470B04Rik | NNNAGAGAGATGGAAACCAG | NGG | 2 | 0.8471 | Tier III |
| 96 | NC_000073.6 | 7 | 98873101 | - | Gm33827 | NNNGGAGAGACAGAGACCAG | NGG | 2 | 0.6222 | Tier III |
| 97 | NC_000075.6 | 9 | 61041827 | - | Gm33699 | NNNGCAGGGATGGAGACCAG | NGG | 2 | 0.5762 | Tier III |
| 98 | NC_000071.6 | 5 | 5485907 | + | Gm30325 | NNNGGAGAGATGGAGACAAA | NGG | 2 | 0.5048 | Tier III |
| 99 | NC_000070.6 | 4 | 127225850 | - | Gm52707 | NNNAGAGAGATGGAGACAAG | NGG | 2 | 0.4846 | Tier III |
| 100 | NC_000071.6 | 5 | 57659993 | - | 4932441J04Rik | NNNGAAGACATGGAGACCAG | NGG | 2 | 0.4667 | Tier III |
| 101 | NC_000072.6 | 6 | 73009485 | + | Gm31547 | NNNGGAGAGATGGGAACCAG | NGG | 2 | 0.4392 | Tier III |
| 102 | NC_000081.6 | 15 | 78023958 | - | Gm46520 | NNNGGAAAGATGGAGACCAC | NGG | 2 | 0.4286 | Tier III |
| 103 | NC_000073.6 | 7 | 98483117 | + | Gm38588 | NNNGGAGGGATGCAGACCAG | NGG | 2 | 0.3088 | Tier III |
| 104 | NC_000072.6 | 6 | 38949819 | - | Gm33767 | NNNGGAGAGCTGAAGACCAG | NGG | 2 | 0.3077 | Tier III |
| 105 | NC_000068.7 | 2 | 172923281 | - | Gm40010 | NNNTGGGAGATGGAGACCAG | NGG | 2 | 0.2597 | Tier III |
| 106 | NC_000075.6 | 9 | 98787111 | - | E330023G01Rik | NNNGGAGACATGTAGACCAG | NGG | 2 | 0.1615 | Tier III |
| 107 | NC_000074.6 | 8 | 121069104 | + | Fendrr | NNNGGAGAGCTGGGGACCAG | NGG | 2 | 0.1556 | Tier III |
| 108 | NC_000074.6 | 8 | 8482225 | - | Gm31135 | NNNGGAGAGATGCAGGCCAG | NGG | 2 | 0.081 | Tier III |
| 109 | NC_000078.6 | 12 | 49463405 | + | Gm34381 | NNNGGAGAGATGGAGGCCAG | NAG | 2 | 0.0499 | Tier III |
| 110 | NC_000070.6 | 4 | 124524057 | - | Gm2164 | NNNGGAGAGATGGAGACAAG | NGA | 2 | 0.0374 | Tier III |
| 111 | NC_000070.6 | 4 | 119703026 | + | Gm35957 | NNNGGAGAGATGGACACGAG | NGG | 2 | 0.0364 | Tier III |
| 112 | NC_000073.6 | 7 | 101352159 | - | Gm35219 | NNNGGAGAGATGGATGCCAG | NGG | 2 | 0.0275 | Tier III |
| 113 | NC_000080.6 | 14 | 61635129 | - | Dleu2 | NNNGGAGAGATGGAGACCAA | NGT | 2 | 0.0151 | Tier III |
| 114 | NC_000079.6 | 13 | 60031693 | + | A530065N20Rik | NNNGGAGAGATGGAGAGCAG | NGC | 2 | 0.0013 | Tier III |
| 115 | NC_000068.7 | 2 | 120036275 | - | Gm28042 | NNNGGAGAGATGGAGCCCAG | NGG | 1 | 0.0 | Tier III |