Construct: sgRNA BRDN0001147211
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGGGACCCAAAAACAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ERN2 (10595)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75846
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
23710185 |
- |
ERN2 |
NNNGGGACCCAAAAACAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
6112875 |
- |
USP42 |
NNNAGGACTCAAAAACAACA |
NGG |
2 |
0.7875 |
Tier II |
| 3 |
NC_000001.11 |
1 |
172217340 |
+ |
DNM3 |
NNNGAGATCCAAAAACAACA |
NGG |
2 |
0.7583 |
Tier II |
| 4 |
NC_000013.11 |
13 |
75688568 |
- |
LMO7 |
NNNGGAAACCAAAAACAACA |
NGG |
2 |
0.65 |
Tier II |
| 5 |
NC_000010.11 |
10 |
13764873 |
+ |
FRMD4A |
NNNGGAACCCAAAAACATCA |
NGG |
2 |
0.5 |
Tier II |
| 6 |
NC_000001.11 |
1 |
227641870 |
+ |
ZNF678 |
NNNGGGACCCAGAAACAACT |
NGG |
2 |
0.4333 |
Tier II |
| 7 |
NC_000002.12 |
2 |
42485381 |
+ |
KCNG3 |
NNNGGGACCAAAAAACAAAA |
NGG |
2 |
0.3714 |
Tier II |
| 8 |
NC_000020.11 |
20 |
32023370 |
- |
CCM2L |
NNNGGGACACAAAAACAAAA |
NGG |
2 |
0.3673 |
Tier II |
| 9 |
NC_000012.12 |
12 |
101113310 |
+ |
ANO4 |
NNNGGGAACCAAAAACAAAA |
NGG |
2 |
0.2786 |
Tier II |
| 10 |
NC_000007.14 |
7 |
22438194 |
- |
STEAP1B |
NNNGGAACCCAACAACAACA |
NGG |
2 |
0.2105 |
Tier II |
| 11 |
NC_000001.11 |
1 |
22729445 |
- |
EPHB2 |
NNNGTGACCCAAAAGCAACA |
NGG |
2 |
0.195 |
Tier II |
| 12 |
NC_000014.9 |
14 |
35855228 |
- |
BRMS1L |
NNNAGGACCCAAAATCAACA |
NGG |
2 |
0.18 |
Tier II |
| 13 |
NC_000019.10 |
19 |
4159137 |
- |
CREB3L3 |
NNNGGGACCCAAATTCAACA |
NGG |
2 |
0.1067 |
Tier II |
| 14 |
NC_000002.12 |
2 |
1117905 |
- |
SNTG2 |
NNNTGGACCCAAAACCAACA |
NGG |
2 |
0.0992 |
Tier II |
| 15 |
NC_000010.11 |
10 |
114708471 |
+ |
ABLIM1 |
NNNGGGACCCCAAAACTACA |
NGG |
2 |
0.0533 |
Tier II |
| 16 |
NC_000008.11 |
8 |
29164050 |
+ |
KIF13B |
NNNGGGACACAAAAACAACA |
NTG |
2 |
0.0334 |
Tier II |
| 17 |
NC_000004.12 |
4 |
48084673 |
+ |
TXK |
NNNGGGACCCAAAAACATCA |
NTG |
2 |
0.0195 |
Tier II |
| 18 |
NC_000012.12 |
12 |
117652765 |
+ |
KSR2 |
NNNGGGACCCAAAAACATCA |
NTG |
2 |
0.0195 |
Tier II |
| 19 |
NC_000013.11 |
13 |
75688568 |
- |
FAM204CP |
NNNGGAAACCAAAAACAACA |
NGG |
2 |
0.65 |
Tier III |
| 20 |
NC_000013.11 |
13 |
83744501 |
+ |
LOC105370286 |
NNNGGGAACCAAAAACAATA |
NGG |
2 |
0.3 |
Tier III |
| 21 |
NC_000008.11 |
8 |
71868622 |
+ |
MSC-AS1 |
NNNGAGACCCAAAACCAACA |
NGG |
2 |
0.2364 |
Tier III |
| 22 |
NC_000008.11 |
8 |
64178377 |
- |
LINC01414 |
NNNGTGTCCCAAAAACAACA |
NGG |
2 |
0.1313 |
Tier III |
| 23 |
NC_000009.12 |
9 |
115807925 |
+ |
LOC105376234 |
NNNGGGACCCAAAAACAAAA |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
122182848 |
- |
Ern2 |
NNNGGAACCCAGAAACAACA |
NGG |
2 |
0.7222 |
Tier I |
| 2 |
NC_000081.6 |
15 |
39824171 |
- |
Dpys |
NNNGGGACCCAGAAGCAACA |
NGG |
2 |
0.4694 |
Tier I |
| 3 |
NC_000067.6 |
1 |
133929809 |
- |
Prelp |
NNNGGGACACAAAGACAACA |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000068.7 |
2 |
14877418 |
- |
Cacnb2 |
NNNGGGACCCAGAAACATCA |
NGG |
2 |
0.3611 |
Tier II |
| 5 |
NC_000069.6 |
3 |
125727568 |
+ |
Ndst4 |
NNNGGGACTCAAAAATAACA |
NGG |
2 |
0.2692 |
Tier II |
| 6 |
NC_000084.6 |
18 |
67595635 |
+ |
Spire1 |
NNNGGGACCCAAAAAAAACA |
NAG |
2 |
0.2593 |
Tier II |
| 7 |
NC_000070.6 |
4 |
42893307 |
- |
Phf24 |
NNNTGGACCCAAAAGCAACA |
NGG |
2 |
0.2364 |
Tier II |
| 8 |
NC_000081.6 |
15 |
72889244 |
+ |
Trappc9 |
NNNGGAACCCAACAACAACA |
NGG |
2 |
0.2105 |
Tier II |
| 9 |
NC_000077.6 |
11 |
62495907 |
- |
Pigl |
NNNGGGACCCCAAAACATCA |
NGG |
2 |
0.2 |
Tier II |
| 10 |
NC_000085.6 |
19 |
48220053 |
+ |
Sorcs3 |
NNNGGGACCCAATAGCAACA |
NGG |
2 |
0.195 |
Tier II |
| 11 |
NC_000082.6 |
16 |
16450898 |
+ |
Fgd4 |
NNNGGGTCCCCAAAACAACA |
NGG |
2 |
0.175 |
Tier II |
| 12 |
NC_000075.6 |
9 |
115960701 |
- |
Gadl1 |
NNNGGGACCCAAAAAAAAGA |
NGG |
2 |
0.125 |
Tier II |
| 13 |
NC_000073.6 |
7 |
45945791 |
- |
Ccdc114 |
NNNGGGACCCAAAAGCCACA |
NGG |
2 |
0.1147 |
Tier II |
| 14 |
NC_000072.6 |
6 |
86985834 |
- |
Aak1 |
NNNAGGACCCAAAAACAACA |
NTG |
2 |
0.0351 |
Tier II |
| 15 |
NC_000079.6 |
13 |
46421573 |
- |
Rbm24 |
NNNGAGACCCAAAAACAACA |
NTG |
2 |
0.0338 |
Tier II |
| 16 |
NC_000067.6 |
1 |
32634471 |
- |
Khdrbs2 |
NNNGGGACCCAACAAGAACA |
NGG |
2 |
0.0324 |
Tier II |
| 17 |
NC_000069.6 |
3 |
76399090 |
- |
Fstl5 |
NNNGGGACCCAAAAAGGACA |
NGG |
2 |
0.0271 |
Tier II |
| 18 |
NC_000077.6 |
11 |
20217076 |
- |
Rab1a |
NNNGGGACCCAAAAAGTACA |
NGG |
2 |
0.0205 |
Tier II |
| 19 |
NC_000081.6 |
15 |
98906160 |
+ |
Lmbr1l |
NNNGGGACCCAACAACAACA |
NTG |
2 |
0.0082 |
Tier II |
| 20 |
NC_000074.6 |
8 |
122275014 |
- |
Gm39269 |
NNNGGGACCCAGAAACATCA |
NGG |
2 |
0.3611 |
Tier III |
| 21 |
NC_000072.6 |
6 |
75438478 |
- |
Gm38835 |
NNNGGTTCCCAAAAACAACA |
NGG |
2 |
0.2917 |
Tier III |
| 22 |
NC_000070.6 |
4 |
114798840 |
+ |
Gm33655 |
NNNGGGACACAACAACAACA |
NGG |
2 |
0.1805 |
Tier III |
| 23 |
NC_000075.6 |
9 |
115960701 |
- |
Gm31234 |
NNNGGGACCCAAAAAAAAGA |
NGG |
2 |
0.125 |
Tier III |
Other clones with same target sequence:
(none)
