Construct: sgRNA BRDN0001147213

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CATACCATGCACAATGACCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLT4 (2324)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75630

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 180629271 + FLT4 NNNACCATGCACAATGACCT NGG 0 1.0 Tier I
2 NC_000003.12 3 49834617 + TRAIP NNNACTATGTACAATGACCT NGG 2 0.8739 Tier II
3 NC_000003.12 3 12558410 - MKRN2 NNNACAGTGCACAATGACCT NGG 2 0.6555 Tier II
4 NC_000003.12 3 12558410 - MKRN2OS NNNACAGTGCACAATGACCT NGG 2 0.6555 Tier II
5 NC_000010.11 10 131926060 + PPP2R2D NNNCCCATGCACAATGACCT NGG 1 0.3529 Tier II
6 NC_000021.9 21 44863525 + PTTG1IP NNNCCCATCCACAATGACCT NGG 2 0.19 Tier II
7 NC_000023.11 X 136509868 - HTATSF1 NNNACCAAGCACAATGTCCT NGG 2 0.1067 Tier II
8 NC_000003.12 3 119187532 - UPK1B NNNACCATCCACAATGTCCT NGG 2 0.0718 Tier II
9 NC_000009.12 9 122151817 + NDUFA8 NNNATCATGCACAATGACCT NCG 2 0.0682 Tier II
10 NC_000010.11 10 80620105 - SH2D4B NNNTCCATGCACAATGACCT NGA 2 0.0442 Tier II
11 NC_000019.10 19 11013823 - SMARCA4 NNNACCATGCCCAAGGACCT NGG 2 0.02 Tier II
12 NC_000009.12 9 76218339 - PCSK5 NNNACCAAGCACAATGACCT NGC 2 0.0178 Tier II
13 NC_000009.12 9 14083268 - NFIB NNNACCCTGCACAATGACCT NTG 2 0.017 Tier II
14 NC_000013.11 13 100672358 + TMTC4 NNNACCATGCACAGTGACCT NGT 2 0.0075 Tier II
15 NC_000005.10 5 25216353 + LINC02211 NNNACCATGGAAAATGACCT NGG 2 0.2778 Tier III
16 NC_000010.11 10 80050635 + TMEM254-AS1 NNNACCATGCACAATGAACC NGG 2 0.049 Tier III
17 NC_000002.12 2 47250110 - EPCAM-DT NNNACCATGCACAATCACCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 49626826 - Flt4 NNNACCGTGCACAATGACCT NTG 2 0.0275 Tier I
2 NC_000070.6 4 108122959 + Scp2 NNNACCACGCACAATGATCT NGG 2 0.6429 Tier II
3 NC_000067.6 1 24189348 + Col9a1 NNNATCATGCACAATAACCT NGG 2 0.6364 Tier II
4 NC_000070.6 4 5716340 - Fam110b NNNACCACCCACAATGACCT NGG 2 0.5385 Tier II
5 NC_000077.6 11 76966051 - Blmh NNNACCATGCACATTAACCT NGG 2 0.5333 Tier II
6 NC_000078.6 12 52895783 - Akap6 NNNACCTTGAACAATGACCT NGG 2 0.3792 Tier II
7 NC_000067.6 1 53531876 + Dnah7a NNNAGCATGCACAAAGACCT NGG 2 0.3474 Tier II
8 NC_000071.6 5 17729367 + Sema3c NNNAGCATGCACAAAGACCT NGG 2 0.3474 Tier II
9 NC_000077.6 11 77167147 - Efcab5 NNNAGCATGCACAAAGACCT NGG 2 0.3474 Tier II
10 NC_000079.6 13 95646089 + Iqgap2 NNNAGCATGCACAAAGACCT NGG 2 0.3474 Tier II
11 NC_000069.6 3 54187747 + Trpc4 NNNACCATCCACAATGATCT NGG 2 0.3462 Tier II
12 NC_000076.6 10 32386444 - Nkain2 NNNACCATCCACAATGATCT NGG 2 0.3462 Tier II
13 NC_000068.7 2 170063848 + Tshz2 NNNAACATGCACAAAGACCT NGG 2 0.3308 Tier II
14 NC_000075.6 9 28418575 + Opcml NNNAACATGCACATTGACCT NGG 2 0.3048 Tier II
15 NC_000070.6 4 63447772 - Whrn NNNTCCATGCACAGTGACCT NGG 2 0.297 Tier II
16 NC_000081.6 15 35559905 - Vps13b NNNACCATCCACAATGAACT NGG 2 0.2899 Tier II
17 NC_000085.6 19 18996675 - Rorb NNNACCATCCACAATGAACT NGG 2 0.2899 Tier II
18 NC_000086.7 X 103409593 - Tsx NNNACCATCCACAATGAACT NGG 2 0.2899 Tier II
19 NC_000072.6 6 77783952 + Ctnna2 NNNACCATTCACAATGAACT NGG 2 0.2872 Tier II
20 NC_000080.6 14 22521074 - Lrmda NNNACCCTACACAATGACCT NGG 2 0.2812 Tier II
21 NC_000067.6 1 130839159 - Pigr NNNTCCATGCCCAATGACCT NGG 2 0.2545 Tier II
22 NC_000072.6 6 111446446 - Grm7 NNNACCTTGCACAAAGACCT NGG 2 0.2533 Tier II
23 NC_000074.6 8 105399248 - Kctd19 NNNACCATCCACAGTGACCT NGG 2 0.2513 Tier II
24 NC_000071.6 5 127685888 - Glt1d1 NNNACCAAGCACAATGACCT NAG 2 0.2074 Tier II
25 NC_000077.6 11 66055367 + Dnah9 NNNACCACGCACAATGAGCT NGG 2 0.1333 Tier II
26 NC_000082.6 16 84818016 - Jam2 NNNACCATGAACAATGTCCT NGG 2 0.1156 Tier II
27 NC_000074.6 8 37602205 + Sgcz NNNACCATGCACAATGGTCT NGG 2 0.1134 Tier II
28 NC_000075.6 9 65845035 + Trip4 NNNAGCATGCACAATGCCCT NGG 2 0.1059 Tier II
29 NC_000077.6 11 57167372 - Gria1 NNNAGCATGCACAATGCCCT NGG 2 0.1059 Tier II
30 NC_000071.6 5 134664822 - Limk1 NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier II
31 NC_000073.6 7 105392673 + Fam160a2 NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier II
32 NC_000074.6 8 108658824 - Zfhx3 NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier II
33 NC_000075.6 9 64669845 + Megf11 NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier II
34 NC_000077.6 11 70508377 + Tm4sf5 NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier II
35 NC_000078.6 12 112788727 + Ahnak2 NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier II
36 NC_000080.6 14 59207431 - Rcbtb1 NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier II
37 NC_000081.6 15 100289037 - Tmprss12 NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier II
38 NC_000067.6 1 153011581 - Nmnat2 NNNACCATGCACAAAGGCCT NGG 2 0.1022 Tier II
39 NC_000085.6 19 50443204 - Sorcs1 NNNACCAGGCACAATGAGCT NGG 2 0.0978 Tier II
40 NC_000080.6 14 23866904 + Kcnma1 NNNACCATGCATAATGGCCT NGG 2 0.095 Tier II
41 NC_000068.7 2 128870883 - Fbln7 NNNACCATGCACCATGACAT NGG 2 0.0902 Tier II
42 NC_000072.6 6 108881569 - Gm6749 NNNAGCATGCACAATGTCCT NGG 2 0.08 Tier II
43 NC_000073.6 7 109944875 + Dennd5a NNNAGCATGCACAATGTCCT NGG 2 0.08 Tier II
44 NC_000083.6 17 6017253 + Synj2 NNNAGCATGCACAATGTCCT NGG 2 0.08 Tier II
45 NC_000086.7 X 151259715 - Wnk3 NNNAGCATGCACAATGTCCT NGG 2 0.08 Tier II
46 NC_000075.6 9 71582080 + Myzap NNNAACATGCACAATGTCCT NGG 2 0.0762 Tier II
47 NC_000076.6 10 28416438 - Ptprk NNNAACATGCACAATGTCCT NGG 2 0.0762 Tier II
48 NC_000067.6 1 119662652 - Ptpn4 NNNACCATCCACAATGAGCT NGG 2 0.0718 Tier II
49 NC_000074.6 8 10521254 - Myo16 NNNACCATCCACAATGAGCT NGG 2 0.0718 Tier II
50 NC_000075.6 9 55735517 + Scaper NNNACCATCCACAATGAGCT NGG 2 0.0718 Tier II
51 NC_000080.6 14 72626921 + Fndc3a NNNACCATCCACAATGAGCT NGG 2 0.0718 Tier II
52 NC_000074.6 8 24016884 - Zmat4 NNNACCATGCACAGTGAGCT NGG 2 0.0622 Tier II
53 NC_000075.6 9 77176741 + Mlip NNNACCATACACAAGGACCT NGG 2 0.0321 Tier II
54 NC_000070.6 4 132855701 - Stx12 NNNAGCATGCACAAGGACCT NGG 2 0.03 Tier II
55 NC_000071.6 5 131728099 + Auts2 NNNAGCATGCACAAGGACCT NGG 2 0.03 Tier II
56 NC_000071.6 5 136750669 - Col26a1 NNNAGCATGCACAAGGACCT NGG 2 0.03 Tier II
57 NC_000080.6 14 13384116 + Synpr NNNACCATGCACAATGGGCT NGG 2 0.0235 Tier II
58 NC_000082.6 16 64490114 + Csnka2ip NNNACCATGCACAATGGGCT NGG 2 0.0235 Tier II
59 NC_000084.6 18 46756336 - Ap3s1 NNNACCATGCACAATGGGCT NGG 2 0.0235 Tier II
60 NC_000068.7 2 73365644 - Gpr155 NNNACCCTGCACAAGGACCT NGG 2 0.0219 Tier II
61 NC_000072.6 6 64291229 - Grid2 NNNACCATGCATAATGACCT NTG 2 0.021 Tier II
62 NC_000071.6 5 107753551 - Evi5 NNNACCATGGACAAGGACCT NGG 2 0.0194 Tier II
63 NC_000070.6 4 82291644 - Nfib NNNACCCTGCACAATGACCT NTG 2 0.017 Tier II
64 NC_000068.7 2 161838962 - Ptprt NNNACCATGCACAATGACCT NGT 1 0.0161 Tier II
65 NC_000071.6 5 121827510 + Sh2b3 NNNACCATGCACAATGCCCC NGG 2 0.016 Tier II
66 NC_000068.7 2 29363095 + Med27 NNNACCATGCACCATGACCT NGA 2 0.0146 Tier II
67 NC_000067.6 1 182472391 - Capn2 NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier II
68 NC_000068.7 2 128061273 + Acoxl NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier II
69 NC_000072.6 6 83210013 - Slc4a5 NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier II
70 NC_000075.6 9 62711888 + Itga11 NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier II
71 NC_000075.6 9 69175773 + Rora NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier II
72 NC_000075.6 9 72081844 + Tcf12 NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier II
73 NC_000082.6 16 76366530 + Nrip1 NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier II
74 NC_000084.6 18 44672661 - Mcc NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier II
75 NC_000071.6 5 124544047 - Tmed2 NNNACCATGCACAATGCCCT NTG 2 0.0069 Tier II
76 NC_000073.6 7 79883227 - Ap3s2 NNNACCATGCACAATGCCCT NTG 2 0.0069 Tier II
77 NC_000083.6 17 42594367 - Opn5 NNNACCATGCACAATGCCCT NTG 2 0.0069 Tier II
78 NC_000083.6 17 24973673 + Cramp1l NNNACCATGCACAATGAGCT NTG 2 0.0052 Tier II
79 NC_000072.6 6 33367475 - Exoc4 NNNACCATGCACAATGTCCT NTG 2 0.0052 Tier II
80 NC_000074.6 8 84025577 + Palm3 NNNACCCTGCACAATCACCT NGG 2 0.0 Tier II
81 NC_000074.6 8 118121045 - Gm25200 NNNATCAGGCACAATGACCT NGG 2 0.4667 Tier III
82 NC_000079.6 13 95646089 + Gm52063 NNNAGCATGCACAAAGACCT NGG 2 0.3474 Tier III
83 NC_000068.7 2 170063848 + A630075F10Rik NNNAACATGCACAAAGACCT NGG 2 0.3308 Tier III
84 NC_000080.6 14 22521074 - 4930405A10Rik NNNACCCTACACAATGACCT NGG 2 0.2812 Tier III
85 NC_000072.6 6 53223482 - Gm4872 NNNACCATGAACAATGGCCT NGG 2 0.1529 Tier III
86 NC_000082.6 16 84818016 - Gm46557 NNNACCATGAACAATGTCCT NGG 2 0.1156 Tier III
87 NC_000079.6 13 23343086 + 4930586N03Rik NNNACCATGCACAAAGCCCT NGG 2 0.1022 Tier III
88 NC_000073.6 7 54594810 - Gm6290 NNNAGCATGCACAATGTCCT NGG 2 0.08 Tier III
89 NC_000068.7 2 144787655 - Gm39951 NNNAGCATGCACAAGGACCT NGG 2 0.03 Tier III
90 NC_000073.6 7 33638678 + Scgb2b23-ps NNNAACATGCACAAGGACCT NGG 2 0.0286 Tier III
91 NC_000082.6 16 8883634 - Gm30310 NNNAACATGCACAAGGACCT NGG 2 0.0286 Tier III
92 NC_000068.7 2 132499444 - 1700026D11Rik NNNACCATGCACAATGGGCT NGG 2 0.0235 Tier III
93 NC_000068.7 2 73365644 - Gm13709 NNNACCCTGCACAAGGACCT NGG 2 0.0219 Tier III
94 NC_000084.6 18 44672661 - A930012L18Rik NNNACCATGCACAAGGCCCT NGG 2 0.0088 Tier III
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Other clones with same target sequence:

(none)