Construct: sgRNA BRDN0001147215
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGATTGAGTTCTTCGAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MKNK2 (2872)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77762
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
2043169 |
- |
MKNK2 |
NNNGATTGAGTTCTTCGAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
70207159 |
- |
PPA1 |
NNNGATTGGGTTCTTAGAGG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000007.14 |
7 |
139800325 |
- |
TBXAS1 |
NNNCATTGAGGTCTTCGAGG |
NGG |
2 |
0.2118 |
Tier II |
| 4 |
NC_000016.10 |
16 |
6679726 |
+ |
RBFOX1 |
NNNGAGTGAGTTCTTTGAGG |
NGG |
2 |
0.1758 |
Tier II |
| 5 |
NC_000005.10 |
5 |
111116264 |
+ |
WDR36 |
NNNGATTGAGTTCTTTGAGG |
NGT |
2 |
0.005 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
125176800 |
- |
Ncapd2 |
NNNGATTGAGTTCTTCGTGA |
NGG |
2 |
0.4688 |
Tier I |
| 2 |
NC_000076.6 |
10 |
80669675 |
- |
Mknk2 |
NNNGATTGAGTTCTTTGAGG |
NGG |
1 |
0.3077 |
Tier I |
| 3 |
NC_000071.6 |
5 |
136126461 |
- |
Lrwd1 |
NNNGATTGAGTACTTCAAGG |
NGG |
2 |
0.7467 |
Tier II |
| 4 |
NC_000075.6 |
9 |
57387638 |
- |
Ppcdc |
NNNGATTGAGTTCTTAGAGT |
NGG |
2 |
0.7 |
Tier II |
| 5 |
NC_000077.6 |
11 |
3178165 |
- |
Sfi1 |
NNNGAGTGAGTTCTTCAAGG |
NGG |
2 |
0.5333 |
Tier II |
| 6 |
NC_000067.6 |
1 |
182778240 |
- |
Susd4 |
NNNGATGGAGTTCATCGAGG |
NGG |
2 |
0.3641 |
Tier II |
| 7 |
NC_000069.6 |
3 |
90731861 |
- |
Pglyrp4 |
NNNGAATGAGTTCTTTGAGG |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000073.6 |
7 |
144127197 |
- |
Shank2 |
NNNGATTGAGTTCATTGAGG |
NGG |
2 |
0.1905 |
Tier II |
| 9 |
NC_000074.6 |
8 |
83914753 |
+ |
Adgrl1 |
NNNGAGTGAGTTCTTCTAGG |
NGG |
2 |
0.1429 |
Tier II |
| 10 |
NC_000079.6 |
13 |
68633650 |
+ |
Adcy2 |
NNNGATGGAGTTCTTGGAGG |
NGG |
2 |
0.0905 |
Tier II |
| 11 |
NC_000086.7 |
X |
66658981 |
- |
Slitrk2 |
NNNGTTTGAGTTCTTCCAGG |
NGG |
2 |
0.0856 |
Tier II |
| 12 |
NT_187064.1 |
|
6277 |
+ |
LOC100861749 |
NNNGAGTGAGTTCTTCAAGG |
NGG |
2 |
0.5333 |
Tier III |
Other clones with same target sequence:
(none)
