Construct: sgRNA BRDN0001147217
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGCTATCCAAGAAGCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BRD3 (8019)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76094
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
134048190 |
- |
BRD3 |
NNNGCTATCCAAGAAGCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
95074141 |
- |
AOPEP |
NNNGGTATCCAAAAAGCACG |
NGG |
2 |
0.5538 |
Tier II |
| 3 |
NC_000002.12 |
2 |
70986971 |
+ |
TEX261 |
NNNGCCATCCAAGAAGCAAG |
NGG |
2 |
0.3896 |
Tier II |
| 4 |
NC_000005.10 |
5 |
133006322 |
- |
ZCCHC10 |
NNNGCTATCCAAGAAGTACC |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000006.12 |
6 |
32321243 |
+ |
TSBP1 |
NNNGCTATCCAAAAAGCAGG |
NGG |
2 |
0.1154 |
Tier II |
| 6 |
NC_000003.12 |
3 |
60018089 |
+ |
FHIT |
NNNGCTGTCCAAGAAGCACG |
NTG |
2 |
0.0275 |
Tier II |
| 7 |
NC_000006.12 |
6 |
32321243 |
+ |
TSBP1-AS1 |
NNNGCTATCCAAAAAGCAGG |
NGG |
2 |
0.1154 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
16224144 |
+ |
Malrd1 |
NNNGCTATCCAACAAGCACT |
NGG |
2 |
0.2947 |
Tier II |
| 2 |
NC_000069.6 |
3 |
127244430 |
+ |
Ank2 |
NNNGCTAACCAATAAGCACG |
NGG |
2 |
0.24 |
Tier II |
| 3 |
NC_000084.6 |
18 |
64584058 |
- |
Atp8b1 |
NNNGCTATCCAAGAAGCTTG |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000075.6 |
9 |
34911104 |
- |
Kirrel3 |
NNNGCTTTCCAAGAAGAACG |
NGG |
2 |
0.2042 |
Tier II |
| 5 |
NC_000068.7 |
2 |
66105184 |
+ |
Galnt3 |
NNNGCTATCCAGGAAGCAGG |
NGG |
2 |
0.0903 |
Tier II |
| 6 |
NC_000082.6 |
16 |
6740154 |
+ |
Rbfox1 |
NNNGCTATCCAGGAAGCAGG |
NGG |
2 |
0.0903 |
Tier II |
| 7 |
NC_000081.6 |
15 |
76742160 |
+ |
Arhgap39 |
NNNGCTATCCAAGAGGCAGG |
NGG |
2 |
0.0813 |
Tier II |
| 8 |
NC_000076.6 |
10 |
18056502 |
+ |
Reps1 |
NNNGGTATCCAAGAAGCAGG |
NGG |
2 |
0.075 |
Tier II |
| 9 |
NC_000085.6 |
19 |
32169857 |
- |
Sgms1 |
NNNGCTATCAAAGAAGCACG |
NGA |
2 |
0.0602 |
Tier II |
Other clones with same target sequence:
(none)
