Construct: sgRNA BRDN0001147227

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGGTGGATCTGATCCGCCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP4K1 (11184)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77610

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 38609654 + MAP4K1 NNNGTGGATCTGATCCGCCG NGG 0 1.0 Tier I
2 NC_000017.11 17 8413246 + NDEL1 NNNGTGGATCTGATCAGCTG NGG 2 0.4615 Tier II
3 NC_000003.12 3 138339105 - LOC124909438 NNNGTGGGTCTGATCCGCCA NGG 2 0.6875 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 28989695 - Map4k1 NNNGTAGATCTGATCCGCCG NGG 1 1.0 Tier I
2 NC_000082.6 16 7051285 - Rbfox1 NNNGTGGATCTGATCCGCAC NGG 2 0.1837 Tier II
3 NC_000080.6 14 30052361 - Cacna1d NNNGTGGTTCTGATCCGCAG NGG 2 0.1837 Tier II
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Other clones with same target sequence:

(none)