Construct: sgRNA BRDN0001147230

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TATTATTATCCCAAGCAACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF1 (6872)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77869

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 71382829 + TAF1 NNNTATTATCCCAAGCAACA NGG 0 1.0 Tier I
2 NC_000009.12 9 32633793 - TAF1L NNNTATTTCCCCAAGCAACA NGG 2 0.3956 Tier I
3 NC_000010.11 10 125138330 + CTBP2 NNNGACTATCCCAAGCAACA NGG 2 0.5682 Tier II
4 NC_000010.11 10 50211167 + ASAH2 NNNTATTATTCCAAGCAAAA NGG 2 0.4034 Tier II
5 NC_000001.11 1 185826319 + HMCN1 NNNAATTATCCAAAGCAACA NGG 2 0.3401 Tier II
6 NC_000001.11 1 44667763 + TMEM53 NNNTATTATCTCAAACAACA NGG 2 0.2896 Tier II
7 NC_000020.11 20 50690562 - RIPOR3 NNNAATTATCCCAAGCAGCA NGG 2 0.1905 Tier II
8 NC_000024.10 Y 12781511 - USP9Y NNNTACTATCCCAAGCTACA NGG 2 0.1212 Tier II
9 NT_187363.1 1 97648 + LOC105379522 NNNTTTTATCCCTAGCAACA NGG 2 0.1091 Tier II
10 NC_000012.12 12 63870439 - SRGAP1 NNNTATTATCCCTAGGAACA NGG 2 0.0462 Tier II
11 NC_000002.12 2 66476105 + MEIS1 NNNTATTATCCCCAGGAACA NGG 2 0.0324 Tier II
12 NC_000008.11 8 50007245 - SNTG1 NNNTATTATCCCATGCAACA NTG 2 0.0208 Tier II
13 NC_000015.10 15 86956066 + AGBL1 NNNAATTATCCCAAGCAACA NTG 2 0.0186 Tier II
14 NC_000007.14 7 46012037 + LOC105375264 NNNTATTATCCTAGGCAACA NGG 2 0.2513 Tier III
15 NT_167218.1 50788 + LOC100287610 NNNTTTTATCCCTAGCAACA NGG 2 0.1091 Tier III
16 NC_000003.12 3 165388557 - LINC01322 NNNTATTATCTCAAGCAACA NAG 2 0.0798 Tier III
17 NC_000009.12 9 104938995 + LOC105376196 NNNTATTATTCCAAGCAACA NTG 2 0.0367 Tier III
18 NC_000015.10 15 99834978 + LOC400464 NNNTATTACCCCAAGCAACA NTG 2 0.036 Tier III
19 NC_000015.10 15 86956066 + LOC102724452 NNNAATTATCCCAAGCAACA NTG 2 0.0186 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 101548228 + Taf1 NNNTACTATCCCAAGCAACA NGG 1 0.9091 Tier I
2 NC_000068.7 2 19246032 + Armc3 NNNTATTATTCCGAGCAACA NGG 2 0.6138 Tier II
3 NC_000085.6 19 30597343 - Prkg1 NNNCACTATCCCAAGCAACA NGG 2 0.5882 Tier II
4 NC_000083.6 17 85003111 - Ppm1b NNNTATAATCCTAAGCAACA NGG 2 0.4712 Tier II
5 NC_000082.6 16 74093144 - Robo2 NNNAATTATCACAAGCAACA NGG 2 0.3571 Tier II
6 NC_000076.6 10 73430270 - Pcdh15 NNNTATTGTCCCAGGCAACA NGG 2 0.3422 Tier II
7 NC_000085.6 19 56363425 + Nrap NNNTTTCATCCCAAGCAACA NGG 2 0.25 Tier II
8 NC_000075.6 9 28711644 - Opcml NNNTATTACCCCAATCAACA NGG 2 0.1319 Tier II
9 NC_000074.6 8 77437610 - Arhgap10 NNNTATCATCCCAAGCCACA NGG 2 0.1213 Tier II
10 NC_000070.6 4 46458744 - Anp32b NNNTATTATGGCAAGCAACA NGG 2 0.0972 Tier II
11 NC_000072.6 6 121058463 + Mical3 NNNTATTATCCCAAGAAACA NTG 2 0.039 Tier II
12 NC_000074.6 8 56078928 - Glra3 NNNTATTTTCCCAAGCAACA NGA 2 0.0298 Tier II
13 NC_000072.6 6 36379604 - 9330158H04Rik NNNTACAATCCCAAGCAACA NGG 2 0.7955 Tier III
14 NC_000085.6 19 56363425 + Gm17197 NNNTTTCATCCCAAGCAACA NGG 2 0.25 Tier III
15 NC_000086.7 X 70030932 - 1700111N16Rik NNNTATAATCCCAAGCAACA NGA 2 0.0608 Tier III
Download CSV

Other clones with same target sequence:

(none)