Construct: sgRNA BRDN0001147230
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TATTATTATCCCAAGCAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAF1 (6872)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77869
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000023.11 |
X |
71382829 |
+ |
TAF1 |
NNNTATTATCCCAAGCAACA |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000009.12 |
9 |
32633793 |
- |
TAF1L |
NNNTATTTCCCCAAGCAACA |
NGG |
2 |
0.3956 |
Tier I |
3 |
NC_000010.11 |
10 |
125138330 |
+ |
CTBP2 |
NNNGACTATCCCAAGCAACA |
NGG |
2 |
0.5682 |
Tier II |
4 |
NC_000010.11 |
10 |
50211167 |
+ |
ASAH2 |
NNNTATTATTCCAAGCAAAA |
NGG |
2 |
0.4034 |
Tier II |
5 |
NC_000001.11 |
1 |
185826319 |
+ |
HMCN1 |
NNNAATTATCCAAAGCAACA |
NGG |
2 |
0.3401 |
Tier II |
6 |
NC_000001.11 |
1 |
44667763 |
+ |
TMEM53 |
NNNTATTATCTCAAACAACA |
NGG |
2 |
0.2896 |
Tier II |
7 |
NC_000020.11 |
20 |
50690562 |
- |
RIPOR3 |
NNNAATTATCCCAAGCAGCA |
NGG |
2 |
0.1905 |
Tier II |
8 |
NC_000024.10 |
Y |
12781511 |
- |
USP9Y |
NNNTACTATCCCAAGCTACA |
NGG |
2 |
0.1212 |
Tier II |
9 |
NT_187363.1 |
1 |
97648 |
+ |
LOC105379522 |
NNNTTTTATCCCTAGCAACA |
NGG |
2 |
0.1091 |
Tier II |
10 |
NC_000012.12 |
12 |
63870439 |
- |
SRGAP1 |
NNNTATTATCCCTAGGAACA |
NGG |
2 |
0.0462 |
Tier II |
11 |
NC_000002.12 |
2 |
66476105 |
+ |
MEIS1 |
NNNTATTATCCCCAGGAACA |
NGG |
2 |
0.0324 |
Tier II |
12 |
NC_000008.11 |
8 |
50007245 |
- |
SNTG1 |
NNNTATTATCCCATGCAACA |
NTG |
2 |
0.0208 |
Tier II |
13 |
NC_000015.10 |
15 |
86956066 |
+ |
AGBL1 |
NNNAATTATCCCAAGCAACA |
NTG |
2 |
0.0186 |
Tier II |
14 |
NC_000007.14 |
7 |
46012037 |
+ |
LOC105375264 |
NNNTATTATCCTAGGCAACA |
NGG |
2 |
0.2513 |
Tier III |
15 |
NT_167218.1 |
|
50788 |
+ |
LOC100287610 |
NNNTTTTATCCCTAGCAACA |
NGG |
2 |
0.1091 |
Tier III |
16 |
NC_000003.12 |
3 |
165388557 |
- |
LINC01322 |
NNNTATTATCTCAAGCAACA |
NAG |
2 |
0.0798 |
Tier III |
17 |
NC_000009.12 |
9 |
104938995 |
+ |
LOC105376196 |
NNNTATTATTCCAAGCAACA |
NTG |
2 |
0.0367 |
Tier III |
18 |
NC_000015.10 |
15 |
99834978 |
+ |
LOC400464 |
NNNTATTACCCCAAGCAACA |
NTG |
2 |
0.036 |
Tier III |
19 |
NC_000015.10 |
15 |
86956066 |
+ |
LOC102724452 |
NNNAATTATCCCAAGCAACA |
NTG |
2 |
0.0186 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000086.7 |
X |
101548228 |
+ |
Taf1 |
NNNTACTATCCCAAGCAACA |
NGG |
1 |
0.9091 |
Tier I |
2 |
NC_000068.7 |
2 |
19246032 |
+ |
Armc3 |
NNNTATTATTCCGAGCAACA |
NGG |
2 |
0.6138 |
Tier II |
3 |
NC_000085.6 |
19 |
30597343 |
- |
Prkg1 |
NNNCACTATCCCAAGCAACA |
NGG |
2 |
0.5882 |
Tier II |
4 |
NC_000083.6 |
17 |
85003111 |
- |
Ppm1b |
NNNTATAATCCTAAGCAACA |
NGG |
2 |
0.4712 |
Tier II |
5 |
NC_000082.6 |
16 |
74093144 |
- |
Robo2 |
NNNAATTATCACAAGCAACA |
NGG |
2 |
0.3571 |
Tier II |
6 |
NC_000076.6 |
10 |
73430270 |
- |
Pcdh15 |
NNNTATTGTCCCAGGCAACA |
NGG |
2 |
0.3422 |
Tier II |
7 |
NC_000085.6 |
19 |
56363425 |
+ |
Nrap |
NNNTTTCATCCCAAGCAACA |
NGG |
2 |
0.25 |
Tier II |
8 |
NC_000075.6 |
9 |
28711644 |
- |
Opcml |
NNNTATTACCCCAATCAACA |
NGG |
2 |
0.1319 |
Tier II |
9 |
NC_000074.6 |
8 |
77437610 |
- |
Arhgap10 |
NNNTATCATCCCAAGCCACA |
NGG |
2 |
0.1213 |
Tier II |
10 |
NC_000070.6 |
4 |
46458744 |
- |
Anp32b |
NNNTATTATGGCAAGCAACA |
NGG |
2 |
0.0972 |
Tier II |
11 |
NC_000072.6 |
6 |
121058463 |
+ |
Mical3 |
NNNTATTATCCCAAGAAACA |
NTG |
2 |
0.039 |
Tier II |
12 |
NC_000074.6 |
8 |
56078928 |
- |
Glra3 |
NNNTATTTTCCCAAGCAACA |
NGA |
2 |
0.0298 |
Tier II |
13 |
NC_000072.6 |
6 |
36379604 |
- |
9330158H04Rik |
NNNTACAATCCCAAGCAACA |
NGG |
2 |
0.7955 |
Tier III |
14 |
NC_000085.6 |
19 |
56363425 |
+ |
Gm17197 |
NNNTTTCATCCCAAGCAACA |
NGG |
2 |
0.25 |
Tier III |
15 |
NC_000086.7 |
X |
70030932 |
- |
1700111N16Rik |
NNNTATAATCCCAAGCAACA |
NGA |
2 |
0.0608 |
Tier III |
Other clones with same target sequence:
(none)