Construct: sgRNA BRDN0001147252

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGAAGGGGCTATGTCAGAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITK (3702)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77449

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 157243761 + ITK NNNAGGGGCTATGTCAGAAG NGG 0 1.0 Tier I
2 NC_000014.9 14 23534422 + ZFHX2 NNNAAGGGGTATGTCAGAAG NGG 2 0.5365 Tier I
3 NC_000017.11 17 8393455 - RNF222 NNNAGTGGCCATGTCAGAAG NGG 2 0.3556 Tier I
4 NC_000003.12 3 49697499 - RNF123 NNNTGGGGCTATGTCAGAAG NGG 1 0.6364 Tier II
5 NC_000003.12 3 183970748 - ABCC5 NNNAAGGGCTATGACAGAAG NGG 2 0.5365 Tier II
6 NC_000014.9 14 23534422 + THTPA NNNAAGGGGTATGTCAGAAG NGG 2 0.5365 Tier II
7 NC_000009.12 9 137427283 + NOXA1 NNNAGGAGCTAGGTCAGAAG NGG 2 0.5 Tier II
8 NC_000006.12 6 13291908 + TBC1D7-LOC100130357 NNNAGTGGCTATGACAGAAG NGG 2 0.4127 Tier II
9 NC_000008.11 8 72024636 + TRPA1 NNNAGGGGATATGTCAGGAG NGG 2 0.3429 Tier II
10 NC_000006.12 6 148967244 + UST NNNAGGGTCTATGTCAGATG NGG 2 0.3365 Tier II
11 NC_000021.9 21 41200945 - BACE2 NNNAGGGCCTAGGTCAGAAG NGG 2 0.3077 Tier II
12 NC_000003.12 3 138204708 - ARMC8 NNNAGGGGCTATTTCAAAAG NGG 2 0.28 Tier II
13 NC_000015.10 15 27045131 + GABRG3 NNNAGGGGTTATTTCAGAAG NGG 2 0.2625 Tier II
14 NC_000010.11 10 119159384 + SFXN4 NNNAGGGGCTGTGTCAGAGG NGG 2 0.2438 Tier II
15 NC_000011.10 11 107564029 - ALKBH8 NNNAGTGGCTTTGTCAGAAG NGG 2 0.2051 Tier II
16 NC_000005.10 5 169099138 - SLIT3 NNNAGGGGCTAGGTCAGAAG NAG 2 0.1296 Tier II
17 NC_000011.10 11 12715643 + TEAD1 NNNAGGGGCTTTGCCAGAAG NGG 2 0.0879 Tier II
18 NC_000006.12 6 41787359 - PRICKLE4 NNNAGGGGCTATCTCAGCAG NGG 2 0.0802 Tier II
19 NC_000002.12 2 23519152 + KLHL29 NNNAGAGGCTATGTCAGAAG NTG 2 0.039 Tier II
20 NC_000020.11 20 61530676 + CDH4 NNNAGGGGCTGTGTCAGAAG NGT 2 0.0105 Tier II
21 NC_000016.10 16 6321347 + RBFOX1 NNNAGGGGCTAGGTCAGAAG NGT 2 0.0081 Tier II
22 NC_000007.14 7 154221511 - DPP6 NNNAGGGGCTATGTGAGAAG NTG 2 0.0019 Tier II
23 NC_000007.14 7 149978118 - ACTR3C NNNAAGGGCTATGGCAGAAG NGG 2 0.0 Tier II
24 NC_000007.14 7 154118730 + DPP6 NNNAAGGGCTATGGCAGAAG NGG 2 0.0 Tier II
25 NC_000003.12 3 73695396 - LOC105377164 NNNTGAGGCTATGTCAGAAG NGG 2 0.6364 Tier III
26 NC_000006.12 6 13291908 + LOC100130357 NNNAGTGGCTATGACAGAAG NGG 2 0.4127 Tier III
27 NC_000019.10 19 54567101 + LOC105372461 NNNTGGGGCTATGACAGAAG NGG 2 0.3939 Tier III
28 NC_000011.10 11 43262508 - HNRNPKP3 NNNAGGGCCTATGACAGAAG NGG 2 0.381 Tier III
29 NC_000008.11 8 72024636 + MSC-AS1 NNNAGGGGATATGTCAGGAG NGG 2 0.3429 Tier III
30 NC_000002.12 2 8247111 + LINC00299 NNNAGGGGCTATGACAGAAC NGG 2 0.2653 Tier III
31 NC_000015.10 15 27045131 + LOC124903449 NNNAGGGGTTATTTCAGAAG NGG 2 0.2625 Tier III
32 NC_000010.11 10 124146960 - LOC105378536 NNNAGGGGCTATTTCAGAGG NGG 2 0.1125 Tier III
33 NC_000002.12 2 23519152 + LOC105374325 NNNAGAGGCTATGTCAGAAG NTG 2 0.039 Tier III
34 NC_000002.12 2 37849234 - PIRAT1 NNNAGGGGCCATGTTAGAAG NGG 2 0.0356 Tier III
35 NC_000022.11 22 24442567 + ADORA2A-AS1 NNNAGGGGCTATGGGAGAAG NGG 2 0.0 Tier III
36 NC_000006.12 6 25133792 + CMAHP NNNAGGGGCTATTGCAGAAG NGG 2 0.0 Tier III
37 NC_000006.12 6 25133792 + LOC124901282 NNNAGGGGCTATTGCAGAAG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 46338139 - Itk NNNAGGGGCGATGTCAGAAG NAG 2 0.1296 Tier I
2 NC_000077.6 11 78117198 + Fam222b NNNAGGAGCTATGTCAGAAG NGG 1 1.0 Tier II
3 NC_000080.6 14 56459250 - Rnf17 NNNAGGAGTTATGTCAGAAG NGG 2 0.875 Tier II
4 NC_000070.6 4 119184666 + Ermap NNNAGAGGCAATGTCAGAAG NGG 2 0.8571 Tier II
5 NC_000081.6 15 9368866 - Ugt3a2 NNNAGGCGCTATGTCAGAAG NGG 1 0.6875 Tier II
6 NC_000081.6 15 55114470 - Deptor NNNAGGGGCTGTGTCAAAAG NGG 2 0.6067 Tier II
7 NC_000072.6 6 136647553 + Plbd1 NNNAGGGGCCATATCAGAAG NGG 2 0.4923 Tier II
8 NC_000077.6 11 47158349 + Sgcd NNNAGGGGCTAAGTCAGATG NGG 2 0.4308 Tier II
9 NC_000074.6 8 48252143 + Tenm3 NNNAGGGGCAAGGTCAGAAG NGG 2 0.4286 Tier II
10 NC_000079.6 13 81791547 + Cetn3 NNNAGGGCCTAGGTCAGAAG NGG 2 0.3077 Tier II
11 NC_000074.6 8 9381574 - Fam155a NNNACGGGCTATGTCAGAGG NGG 2 0.2946 Tier II
12 NC_000080.6 14 32862435 - Vstm4 NNNAGGGGCCATGTCAGATG NGG 2 0.2872 Tier II
13 NC_000084.6 18 80451798 - Ctdp1 NNNTGGGGCTCTGTCAGAAG NGG 2 0.2545 Tier II
14 NC_000080.6 14 122264433 - Clybl NNNAGGGGCTATGTCAGCAG NGG 1 0.1905 Tier II
15 NC_000070.6 4 137747855 + Alpl NNNAGGGGCTGTGTCAGCAG NGG 2 0.1238 Tier II
16 NC_000067.6 1 32230313 - Khdrbs2 NNNAGGGGCTATGTCACTAG NGG 2 0.1176 Tier II
17 NC_000081.6 15 9313108 - Ugt3a1 NNNAGGGGCTATGTCAGCTG NGG 2 0.1026 Tier II
18 NC_000077.6 11 121715686 - Metrnl NNNATGGGCTATGTCAGCAG NGG 2 0.0571 Tier II
19 NC_000082.6 16 60268464 + Epha6 NNNAGGGGCTATGTGAGAAA NGG 2 0.0469 Tier II
20 NC_000073.6 7 65908211 - Pcsk6 NNNAGGGGCTGTGTGAGAAG NGG 2 0.0325 Tier II
21 NC_000071.6 5 36605781 + D5Ertd579e NNNAGGTGCTATGTGAGAAG NGG 2 0.0286 Tier II
22 NC_000072.6 6 33772681 - Exoc4 NNNAGGGGCTATGTGAGAGG NGG 2 0.0188 Tier II
23 NC_000076.6 10 5499929 - Syne1 NNNAGGGGCTAAGTCAGAAG NGC 2 0.0178 Tier II
24 NC_000075.6 9 45541943 - Dscaml1 NNNAGGGGCTTTGTCAGAAG NGT 2 0.005 Tier II
25 NC_000075.6 9 75480843 + Gm7972 NNNAGGGGCCATGTCTGAAG NGG 2 0.0 Tier II
26 NC_000078.6 12 30322664 + Sntg2 NNNAGGGGCTATGGAAGAAG NGG 2 0.0 Tier II
27 NC_000076.6 10 61244472 - Adamts14 NNNCGGGGCTATGGCAGAAG NGG 2 0.0 Tier II
28 NC_000071.6 5 150002114 - Gm21048 NNNAGAGGCTATGTCAGATG NGG 2 0.5385 Tier III
29 NC_000070.6 4 24881462 + Gm46848 NNNAGGGACCATGTCAGAAG NGG 2 0.5333 Tier III
30 NC_000081.6 15 7512954 + Gm41264 NNNAGGGGATATTTCAGAAG NGG 2 0.2571 Tier III
31 NC_000081.6 15 9320318 - Gm2360 NNNAGGGGCTATGTCAGCTG NGG 2 0.1026 Tier III
32 NC_000068.7 2 70554489 - Gad1os NNNAGGGGCTCTGTAAGAAG NGG 2 0.0889 Tier III
33 NC_000072.6 6 69684115 - Igk NNNAGGGGCTATGTCAAAAG NTG 2 0.0364 Tier III
34 NC_000076.6 10 57120186 - Gm40652 NNNAGGGGCTTTGTGAGAAG NGG 2 0.0154 Tier III
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Other clones with same target sequence:

(none)