Construct: sgRNA BRDN0001147254

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGAAAAGGCATCTCCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL4A3BP (10087)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77850

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 75400250 + CERT1 NNNGAAAAGGCATCTCCAGA NGG 0 1.0 Tier I
2 NC_000012.12 12 15551662 + PTPRO NNNGAAAAAGCATCTGCAGA NGG 2 0.0989 Tier I
3 NC_000001.11 1 154750464 - KCNN3 NNNGAGAAGGCATCTCCAGA NGG 1 0.7143 Tier II
4 NC_000003.12 3 140686078 - TRIM42 NNNAAAAAGGCATCTCCAGG NGG 2 0.6882 Tier II
5 NC_000023.11 X 80027054 + TBX22 NNNGAGAAGGCAACTCCAGA NGG 2 0.4945 Tier II
6 NC_000010.11 10 104063731 + COL17A1 NNNGAAAAGTCATCTCAAGA NGG 2 0.3792 Tier II
7 NC_000006.12 6 147282442 - STXBP5 NNNGAAAAGGAATCTCCACA NGG 2 0.3362 Tier II
8 NC_000006.12 6 42624788 + UBR2 NNNGAAAAGGCATCTCAAAA NGG 2 0.3333 Tier II
9 NC_000015.10 15 54044130 - UNC13C NNNGAAAAGGCAACTCCACA NGG 2 0.3103 Tier II
10 NC_000011.10 11 62350204 - ASRGL1 NNNGAAAAGCCATTTCCAGA NGG 2 0.2933 Tier II
11 NC_000003.12 3 38055554 + DLEC1 NNNGTAAAGGCATTTCCAGA NGG 2 0.2667 Tier II
12 NC_000009.12 9 121088102 - CNTRL NNNGAAAAGGGATCTCCAGA NGG 1 0.25 Tier II
13 NC_000007.14 7 112111248 + DOCK4 NNNGAAAAGCCATCTCCAGT NGG 2 0.24 Tier II
14 NC_000017.11 17 79239934 - RBFOX3 NNNGAAAAGGCCTCTCCAGG NGG 2 0.2012 Tier II
15 NC_000020.11 20 45843146 + ACOT8 NNNGAAAAGGCATCCCCAAA NGG 2 0.1948 Tier II
16 NC_000020.11 20 45843146 + SNX21 NNNGAAAAGGCATCCCCAAA NGG 2 0.1948 Tier II
17 NC_000007.14 7 70093519 + AUTS2 NNNGAAAAGCCATCTCTAGA NGG 2 0.1867 Tier II
18 NC_000023.11 X 124931215 - TENM1 NNNGAAAATGCATCTTCAGA NGG 2 0.1641 Tier II
19 NC_000011.10 11 35165310 + CD44 NNNGAAAAGGCAGCACCAGA NGG 2 0.151 Tier II
20 NC_000010.11 10 97984887 + CRTAC1 NNNGAAAAGGCAGCTCAAGA NGG 2 0.1217 Tier II
21 NC_000017.11 17 67682546 + PITPNC1 NNNGAAATGGCCTCTCCAGA NGG 2 0.1128 Tier II
22 NC_000008.11 8 22743577 - PEBP4 NNNGAAAAGGCAGCTCCGGA NGG 2 0.1043 Tier II
23 NC_000014.9 14 21526553 - SALL2 NNNGCAAAGGCATCTGCAGA NGG 2 0.0769 Tier II
24 NC_000002.12 2 161177745 + TANK NNNGAAAAGGCATCTACAGA NTG 2 0.039 Tier II
25 NC_000002.12 2 31076445 - GALNT14 NNNGAAAAGGGATCTCCAGA NGA 2 0.0174 Tier II
26 NC_000013.11 13 40574233 - FOXO1 NNNGAAAAGGCATCTCCAAA NGT 2 0.0115 Tier II
27 NC_000011.10 11 71208969 + SHANK2 NNNGAAAAGGCATCTGCAGA NGT 2 0.0025 Tier II
28 NC_000002.12 2 74008151 - TET3 NNNGAAAAGTCATGTCCAGA NGG 2 0.0 Tier II
29 NC_000020.11 20 33204863 + BPIFA4P NNNAAGAAGGCATCTCCAGA NGG 2 0.6429 Tier III
30 NC_000021.9 21 22081717 + LINC01687 NNNGAAAAGGCATCTCAAAA NGG 2 0.3333 Tier III
31 NC_000020.11 20 52535140 + LOC105372666 NNNGAAAAGCCAACTCCAGA NGG 2 0.2769 Tier III
32 NC_000018.10 18 47936370 - LOC124904297 NNNGAAAAAGCTTCTCCAGA NGG 2 0.2143 Tier III
33 NC_000009.12 9 2561052 + VLDLR-AS1 NNNGAAAAGCCTTCTCCAGA NGG 2 0.1333 Tier III
34 NC_000008.11 8 29737145 - LINC00589 NNNGAAAAGGCATCACCAGC NGG 2 0.1316 Tier III
35 NC_000017.11 17 67682546 + LOC101928045 NNNGAAATGGCCTCTCCAGA NGG 2 0.1128 Tier III
36 NC_000008.11 8 22743577 - LOC124901908 NNNGAAAAGGCAGCTCCGGA NGG 2 0.1043 Tier III
37 NC_000012.12 12 15551662 + LOC105369672 NNNGAAAAAGCATCTGCAGA NGG 2 0.0989 Tier III
38 NC_000009.12 9 117831343 + LOC105376244 NNNGAAAAGGCCTCTCCCGA NGG 2 0.0501 Tier III
39 NC_000004.12 4 123536633 - LOC105377405 NNNTAAAAGGCATCTCCAGA NGA 2 0.0253 Tier III
40 NC_000004.12 4 66974817 + LOC105377262 NNNGAACAGGCATCTCCAGA NTG 2 0.017 Tier III
41 NC_000013.11 13 69296336 - LINC00383 NNNTAAAAGGCATCTCCAGA NTG 2 0.0142 Tier III
42 NC_000001.11 1 146374537 - SEC22B4P NNNGAAAAGGCATCTCAAGA NGC 2 0.0104 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 119381902 - Ino80 NNNGAAAAGGTATCTCCAGA NGG 1 0.3077 Tier I
2 NC_000079.6 13 96616505 - Cert1 NNNGAAAAGGTGTCTCCAGA NGG 2 0.2222 Tier I
3 NC_000076.6 10 78459239 + Pdxk NNNGGAGAGGCATCTCCAGA NGG 2 0.5082 Tier II
4 NC_000078.6 12 52742519 + Akap6 NNNGAAAAAGCATTTCCAGA NGG 2 0.4714 Tier II
5 NC_000068.7 2 17558540 + Nebl NNNGCTAAGGCATCTCCAGA NGG 2 0.3571 Tier II
6 NC_000084.6 18 66263732 + Ccbe1 NNNGAAAAGGCATCTCCTGT NGG 2 0.3 Tier II
7 NC_000067.6 1 6776103 + St18 NNNGAAACAGCATCTCCAGA NGG 2 0.2755 Tier II
8 NC_000074.6 8 117378604 - Cmip NNNGTAAAGGCATTTCCAGA NGG 2 0.2667 Tier II
9 NC_000076.6 10 26816400 + Arhgap18 NNNGAAACCGCATCTCCAGA NGG 2 0.2308 Tier II
10 NC_000078.6 12 116663872 + Ptprn2 NNNGAAAAGGCATCTCCAAA NAG 2 0.1852 Tier II
11 NC_000070.6 4 42227183 - Ccl21b NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier II
12 NC_000070.6 4 147486376 - Zfp982 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier II
13 NC_000076.6 10 36614558 - Hs3st5 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier II
14 NC_000079.6 13 62552922 - Zfp934 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier II
15 NC_000080.6 14 87086092 + Diaph3 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier II
16 NC_000081.6 15 4560021 + Plcxd3 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier II
17 NC_000081.6 15 22700523 - Cdh18 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier II
18 NC_000083.6 17 6576751 - Gm29721 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier II
19 NC_000083.6 17 70649388 + Dlgap1 NNNGAAAAGACATCTGCAGA NGG 2 0.1436 Tier II
20 NC_000078.6 12 99422883 - Foxn3 NNNGAACAGGCATCCCCAGA NGG 2 0.1193 Tier II
21 NC_000071.6 5 19922866 - Magi2 NNNGAAAAGGCATCTCCTGC NGG 2 0.1136 Tier II
22 NC_000071.6 5 113902886 + Coro1c NNNGAATAGGCATCTCCAGA NAG 2 0.1134 Tier II
23 NC_000070.6 4 118232891 + Ptprf NNNGACAAGGCATCTCCAGC NGG 2 0.1033 Tier II
24 NC_000083.6 17 12622207 + Slc22a2 NNNGAAAAGGCATCTCCACC NGG 2 0.1019 Tier II
25 NC_000085.6 19 28840087 - Slc1a1 NNNGAAAAGGCAGATCCAGA NGG 2 0.0913 Tier II
26 NC_000081.6 15 37338867 - Grhl2 NNNGAAAAGGCTTCTCCAGA NAG 2 0.0864 Tier II
27 NC_000084.6 18 30310820 + Pik3c3 NNNGAAAAGGTAGCTCCAGA NGG 2 0.0803 Tier II
28 NC_000068.7 2 6880565 - Celf2 NNNGAAATGGCATCTGCAGA NGG 2 0.0659 Tier II
29 NC_000073.6 7 25071597 + Grik5 NNNGTAAAGGCATCTCCAGA NGA 2 0.0253 Tier II
30 NC_000073.6 7 83966535 + Cemip NNNGAAAAGGCTTCGCCAGA NGG 2 0.0167 Tier II
31 NC_000081.6 15 39908078 + Lrp12 NNNGAAAAGGGATCGCCAGA NGG 2 0.0125 Tier II
32 NC_000068.7 2 174611615 - Zfp831 NNNGAAAGGGCATCTCCAGA NGT 2 0.0118 Tier II
33 NC_000081.6 15 82093834 - Mei1 NNNGAAAAGGCATCTTCAGA NGT 2 0.005 Tier II
34 NC_000085.6 19 7525729 + Atl3 NNNGAAAAGGCAAGTCCAGA NGG 2 0.0 Tier II
35 NC_000067.6 1 45971969 + Gm9496 NNNGGAAAGGCATCTCCAGA NGG 1 0.72 Tier III
36 NC_000075.6 9 72408763 + BC065403 NNNGAAATGGCATCTCCAGA NGG 1 0.4286 Tier III
37 NC_000075.6 9 72408763 + Gm27230 NNNGAAATGGCATCTCCAGA NGG 1 0.4286 Tier III
38 NC_000071.6 5 134769622 - Gm30003 NNNCAAAAGGCAACTCCAGA NGG 2 0.3665 Tier III
39 NC_000067.6 1 103050211 - Gm18728 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier III
40 NC_000068.7 2 126529195 - Gm26697 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier III
41 NC_000079.6 13 62552922 - Gm38639 NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier III
42 NC_000084.6 18 17070016 - 1700001G01Rik NNNGAAAAAGCAGCTCCAGA NGG 2 0.1677 Tier III
43 NC_000085.6 19 28840087 - Gm52404 NNNGAAAAGGCAGATCCAGA NGG 2 0.0913 Tier III
44 NC_000076.6 10 50481157 + Gm6991 NNNGAAAAGGCATCTCCAAA NTG 2 0.0278 Tier III
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Other clones with same target sequence:

(none)