Construct: sgRNA BRDN0001147257
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTGTGGCAGCGTCAACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MET (4233)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76063
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
116699495 |
+ |
MET |
NNNTGTGGCAGCGTCAACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
10183427 |
+ |
RRM2 |
NNNTGTGACAGAGTCAACAG |
NGG |
2 |
0.7143 |
Tier II |
| 3 |
NC_000008.11 |
8 |
73090704 |
- |
SBSPON |
NNNTGTGGCAGAGTCAACAG |
NGG |
1 |
0.7143 |
Tier II |
| 4 |
NC_000022.11 |
22 |
41507699 |
- |
ACO2 |
NNNTGTGGCAACCTCAACAG |
NGG |
2 |
0.4211 |
Tier II |
| 5 |
NC_000001.11 |
1 |
107402508 |
- |
NTNG1 |
NNNTGTGGCAGACTCAACAG |
NGG |
2 |
0.3008 |
Tier II |
| 6 |
NC_000003.12 |
3 |
58999398 |
+ |
CFAP20DC |
NNNAGTGGCAGTGTCAACAG |
NGG |
2 |
0.2564 |
Tier II |
| 7 |
NC_000007.14 |
7 |
130539706 |
+ |
COPG2 |
NNNTGAGGCAGCGCCAACAG |
NGG |
2 |
0.2476 |
Tier II |
| 8 |
NC_000019.10 |
19 |
3508980 |
- |
FZR1 |
NNNTGTGGCAGTGCCAACAG |
NGG |
2 |
0.1538 |
Tier II |
| 9 |
NC_000011.10 |
11 |
76796801 |
+ |
TSKU |
NNNTGTGGCAGCGTCACCAC |
NGG |
2 |
0.0756 |
Tier II |
| 10 |
NC_000022.11 |
22 |
45327659 |
+ |
FAM118A |
NNNTGTGGCAGCGTCACCGG |
NGG |
2 |
0.0662 |
Tier II |
| 11 |
NC_000020.11 |
20 |
63959717 |
+ |
ZNF512B |
NNNTGTGGCAGCCTTAACAG |
NGG |
2 |
0.0281 |
Tier II |
| 12 |
NC_000022.11 |
22 |
24170659 |
+ |
CABIN1 |
NNNTGTGGCAGAGTCCACAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000003.12 |
3 |
25509390 |
- |
RARB |
NNNTGTGGCAGCAGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000006.12 |
6 |
15524355 |
- |
DTNBP1 |
NNNTGTGGCAGCTGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000020.11 |
20 |
41472888 |
- |
CHD6 |
NNNTGTGGCAGGGGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000018.10 |
18 |
46482833 |
+ |
LOXHD1 |
NNNTGTGGGAGCGTCCACAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000006.12 |
6 |
139833863 |
+ |
FILNC1 |
NNNTGTGGCAGCATCAACAC |
NGG |
2 |
0.3956 |
Tier III |
| 18 |
NC_000003.12 |
3 |
58999398 |
+ |
CFAP20DC-AS1 |
NNNAGTGGCAGTGTCAACAG |
NGG |
2 |
0.2564 |
Tier III |
| 19 |
NC_000008.11 |
8 |
137966316 |
- |
LOC401478 |
NNNTGTGGCAGTGTCAACAG |
NGT |
2 |
0.0087 |
Tier III |
| 20 |
NC_000023.11 |
X |
76029606 |
+ |
ARL5AP5 |
NNNTGTGGCAGCGGCAGCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
17491651 |
+ |
Met |
NNNTGTGGCAGTGTCAACAG |
NGG |
1 |
0.5385 |
Tier I |
| 2 |
NC_000077.6 |
11 |
46137626 |
+ |
Adam19 |
NNNGGTGGCAGCGTCGACAG |
NGG |
2 |
0.1202 |
Tier I |
| 3 |
NC_000076.6 |
10 |
79762312 |
+ |
Rnf126 |
NNNTGTGGCAGCGTGAACAG |
NTG |
2 |
0.0019 |
Tier I |
| 4 |
NC_000075.6 |
9 |
105362062 |
+ |
Nek11 |
NNNTGTGACAGCATCAACAG |
NGG |
2 |
0.9231 |
Tier II |
| 5 |
NC_000068.7 |
2 |
127247397 |
- |
Ciao1 |
NNNTGTGCCAGCGTCAACAG |
NGG |
1 |
0.6154 |
Tier II |
| 6 |
NC_000075.6 |
9 |
65853539 |
+ |
Trip4 |
NNNTGTGTCAGAGTCAACAG |
NGG |
2 |
0.4464 |
Tier II |
| 7 |
NC_000072.6 |
6 |
115909287 |
+ |
Ift122 |
NNNTGAGGCAGCTTCAACAG |
NGG |
2 |
0.26 |
Tier II |
| 8 |
NC_000071.6 |
5 |
122462109 |
+ |
Atp2a2 |
NNNTTTGGCAGCCTCAACAG |
NGG |
2 |
0.1263 |
Tier II |
| 9 |
NC_000067.6 |
1 |
43164093 |
- |
Fhl2 |
NNNTGTGGTAGCGTCATCAG |
NGG |
2 |
0.1167 |
Tier II |
| 10 |
NC_000071.6 |
5 |
125058522 |
+ |
Ncor2 |
NNNCGTGGCAGCGTCAGCAG |
NGG |
2 |
0.1142 |
Tier II |
| 11 |
NC_000077.6 |
11 |
118562826 |
- |
Rbfox3 |
NNNTGTGGCAGTGTCAAGAG |
NGG |
2 |
0.0718 |
Tier II |
| 12 |
NC_000084.6 |
18 |
7891921 |
+ |
Wac |
NNNTGTGGCATCGTCAGCAG |
NGG |
2 |
0.0679 |
Tier II |
| 13 |
NC_000080.6 |
14 |
66647030 |
- |
Adra1a |
NNNTGTGGCAGGGTCATCAG |
NGG |
2 |
0.0593 |
Tier II |
| 14 |
NC_000077.6 |
11 |
103824469 |
- |
Nsf |
NNNTGTGGCAGCGTCACCAG |
NAG |
2 |
0.0458 |
Tier II |
| 15 |
NC_000077.6 |
11 |
20629814 |
- |
Sertad2 |
NNNAGTGGCAGCGTCAACAG |
NTG |
2 |
0.0186 |
Tier II |
| 16 |
NC_000085.6 |
19 |
10430495 |
+ |
Syt7 |
NNNTGCGGCAGCGGCAACAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000081.6 |
15 |
31242876 |
- |
Dap |
NNNTGGGGCAGCGTCCACAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000068.7 |
2 |
170058794 |
- |
Tshz2 |
NNNTGTGGCAGCGTCTACAC |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000075.6 |
9 |
22592569 |
+ |
Bbs9 |
NNNTGTGGCATCGTCTACAG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000069.6 |
3 |
94721166 |
- |
Gm15264 |
NNNTGTGGAAGCATCAACAG |
NGG |
2 |
0.7912 |
Tier III |
| 21 |
NC_000080.6 |
14 |
67482251 |
- |
Gm38409 |
NNNTGTGGCAGAGTAAACAG |
NGG |
2 |
0.1587 |
Tier III |
| 22 |
NC_000071.6 |
5 |
105654673 |
- |
Gm32276 |
NNNTGTGGCAGGGTCCACAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
