Construct: sgRNA BRDN0001147273

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CACCAGAAGTAATTTCAGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAA1 (5562)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76254

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 40769421 - PRKAA1 NNNCAGAAGTAATTTCAGGA NGG 0 1.0 Tier I
2 NC_000014.9 14 79688984 + NRXN3 NNNCAGAAGTAATTTAAGAA NGG 2 0.7143 Tier II
3 NC_000008.11 8 107349421 + ANGPT1 NNNCAGAAGAAATTTCAGGG NGG 2 0.6555 Tier II
4 NC_000020.11 20 32711638 - COMMD7 NNNCAGAGGTAACTTCAGGA NGG 2 0.5789 Tier II
5 NC_000012.12 12 131904852 - ULK1 NNNCAGAAATAACTTCAGGA NGG 2 0.5075 Tier II
6 NC_000003.12 3 124924648 + MUC13 NNNCATAGGTAATTTCAGGA NGG 2 0.4889 Tier II
7 NC_000009.12 9 8806608 - PTPRD NNNTAGAATTAATTTCAGGA NGG 2 0.4267 Tier II
8 NC_000017.11 17 2935605 - RAP1GAP2 NNNTAGAATTAATTTCAGGA NGG 2 0.4267 Tier II
9 NC_000021.9 21 33898576 + ITSN1 NNNCAGAAATGATTTCAGGA NGG 2 0.4179 Tier II
10 NC_000021.9 21 42263134 - ABCG1 NNNTAGAAGGAATTTCAGGA NGG 2 0.4 Tier II
11 NC_000007.14 7 8664420 - NXPH1 NNNCAGAAGCAATTTCAGAA NGG 2 0.381 Tier II
12 NC_000018.10 18 27008359 + CHST9 NNNAAGAAGTAATTTCAGCA NGG 2 0.3775 Tier II
13 NC_000015.10 15 30979635 + MTMR10 NNNCAGAAGTAATAACAGGA NGG 2 0.3584 Tier II
14 NC_000012.12 12 41412362 + PDZRN4 NNNGAGAAGTGATTTCAGGA NGG 2 0.325 Tier II
15 NC_000014.9 14 56240158 + PELI2 NNNCAGAAGTAGTTTCAGCA NGG 2 0.3238 Tier II
16 NC_000009.12 9 109716745 + PALM2AKAP2 NNNCAGAAGCAATTACAGGA NGG 2 0.3088 Tier II
17 NC_000007.14 7 90888500 + CDK14 NNNCAAAAGTTATTTCAGGA NGG 2 0.3077 Tier II
18 NC_000014.9 14 61345469 + PRKCH NNNCAGCAGTAATTTCATGA NGG 2 0.2917 Tier II
19 NC_000016.10 16 67956940 + SLC12A4 NNNCAGTAGTGATTTCAGGA NGG 2 0.2844 Tier II
20 NC_000014.9 14 47669306 - MDGA2 NNNCAAAAGTAATTCCAGGA NGG 2 0.2727 Tier II
21 NC_000017.11 17 46813451 + WNT3 NNNCAGATGTAATATCAGGA NGG 2 0.2653 Tier II
22 NC_000017.11 17 46813451 + LRRC37A2 NNNCAGATGTAATATCAGGA NGG 2 0.2653 Tier II
23 NC_000006.12 6 118118760 + SLC35F1 NNNCAGAACTCATTTCAGGA NGG 2 0.2154 Tier II
24 NC_000006.12 6 159175521 - FNDC1 NNNCAGAAGTAAACTCAGGA NGG 2 0.1978 Tier II
25 NC_000001.11 1 89174259 + GBP7 NNNCAGGAGTAATTTCAGGA NAG 2 0.183 Tier II
26 NC_000007.14 7 15301924 - AGMO NNNCAGAAGTAACTTCAGGC NGG 2 0.1794 Tier II
27 NC_000015.10 15 41184956 - EXD1 NNNCAGAAGTGATTTCAGGC NGG 2 0.1477 Tier II
28 NC_000011.10 11 70098925 + ANO1 NNNCAGAAGCAATTCCAGGA NGG 2 0.1455 Tier II
29 NC_000008.11 8 106319199 + OXR1 NNNCAGAAGTAATTTCGGGT NGG 2 0.1059 Tier II
30 NC_000008.11 8 140119355 + TRAPPC9 NNNCAGAAGTCCTTTCAGGA NGG 2 0.1053 Tier II
31 NC_000021.9 21 45695688 - PCBP3 NNNCAGAAGTAATTTGAGTA NGG 2 0.1026 Tier II
32 NC_000018.10 18 45031833 + SETBP1 NNNCAGAAGTATGTTCAGGA NGG 2 0.087 Tier II
33 NC_000008.11 8 138192484 - FAM135B NNNCAGAAGCAATTTGAGGA NGG 2 0.0821 Tier II
34 NC_000001.11 1 10435874 - CENPS NNNCAGAAGTATTTTCAGGC NGG 2 0.0758 Tier II
35 NC_000001.11 1 10435874 - CENPS-CORT NNNCAGAAGTATTTTCAGGC NGG 2 0.0758 Tier II
36 NC_000010.11 10 82083361 + NRG3 NNNCAGAAGTAATTTAAGGA NGA 2 0.0694 Tier II
37 NC_000002.12 2 114752482 - DPP10 NNNCACAAGTAATTTCAGGA NGA 2 0.0473 Tier II
38 NC_000007.14 7 8498531 + NXPH1 NNNCAGAAGGAATTTCAGGA NGA 2 0.0347 Tier II
39 NC_000002.12 2 230793871 - CAB39 NNNCAGTAGTAATTTCAGGA NGA 2 0.0304 Tier II
40 NC_000004.12 4 517543 + PIGG NNNAAGAAGTAATTTCAGGA NGC 2 0.0187 Tier II
41 NC_000010.11 10 35155070 + CREM NNNCAGAGGTAATTTCAGGA NGT 2 0.0118 Tier II
42 NC_000009.12 9 105620113 + FKTN NNNCAGAAATAATTTCAGGA NGT 2 0.0104 Tier II
43 NC_000004.12 4 53384962 + FIP1L1 NNNCAGAAGTCATTTCAGGA NGC 2 0.0089 Tier II
44 NC_000005.10 5 40769421 - LOC124900968 NNNCAGAAGTAATTTCAGGA NGG 0 1.0 Tier III
45 NC_000023.11 X 128689962 + LOC107985698 NNNCAAAAATAATTTCAGGA NGG 2 0.6429 Tier III
46 NC_000012.12 12 129974669 - LOC105370076 NNNTTGAAGTAATTTCAGGA NGG 2 0.2909 Tier III
47 NC_000003.12 3 181064028 - SOX2-OT NNNCAGATGGAATTTCAGGA NGG 2 0.2143 Tier III
48 NC_000005.10 5 36244290 - LOC124900962 NNNCAGAAGTAGTCTCAGGA NGG 2 0.2063 Tier III
49 NC_000005.10 5 126055552 + LOC124901056 NNNCAGAAGGCATTTCAGGA NGG 2 0.2 Tier III
50 NC_000004.12 4 100848071 - LINC01218 NNNCAGAAGTAGTTCCAGGA NGG 2 0.197 Tier III
51 NC_000013.11 13 78197974 - OBI1-AS1 NNNCAGCTGTAATTTCAGGA NGG 2 0.1875 Tier III
52 NC_000001.11 1 89174259 + LOC105378842 NNNCAGGAGTAATTTCAGGA NAG 2 0.183 Tier III
53 NC_000007.14 7 15301924 - LOC124901592 NNNCAGAAGTAACTTCAGGC NGG 2 0.1794 Tier III
54 NC_000003.12 3 164565166 - LOC105374191 NNNCAGAAGTTATTTTAGGA NGG 2 0.0947 Tier III
55 NC_000003.12 3 117070844 - LOC124909415 NNNCAGAAGTAATTACAGGA NGA 2 0.0402 Tier III
56 NC_000002.12 2 51231361 + NRXN1-DT NNNCAGCAGTAATTTCAGGA NTG 2 0.017 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 5170012 + Prkaa1 NNNCAGAAGTCATTTCAGGA NGG 1 0.4 Tier I
2 NC_000067.6 1 58016765 + Sgo2a NNNCAGAAGTAATTTCTGGA NTG 2 0.0052 Tier I
3 NC_000081.6 15 78965183 + Triobp NNNCAGAAGAAATTTAAGGA NGG 2 0.8571 Tier II
4 NC_000081.6 15 20483956 + Cdh12 NNNCAGAAGAAACTTCAGGA NGG 2 0.6767 Tier II
5 NC_000073.6 7 66932282 - Adamts17 NNNAAGAAGTAATTTCAGGG NGG 2 0.644 Tier II
6 NC_000086.7 X 73539287 - Atp2b3 NNNCAGAAGAAATTTCAAGA NGG 2 0.5934 Tier II
7 NC_000085.6 19 57728022 - Atrnl1 NNNAAGAAGTAATTTCAGTA NGG 2 0.5614 Tier II
8 NC_000075.6 9 94948456 + Slc9a9 NNNCAGAAGCAATTTAAGGA NGG 2 0.5333 Tier II
9 NC_000078.6 12 104714020 - Dicer1 NNNCAGAAGCAATTTCAGGA NGG 1 0.5333 Tier II
10 NC_000079.6 13 92850644 - Mtx3 NNNCAGAAATAACTTCAGGA NGG 2 0.5075 Tier II
11 NC_000068.7 2 67913414 - B3galt1 NNNCAGAAATAATTTCAGGG NGG 2 0.4916 Tier II
12 NC_000069.6 3 33095558 + Pex5l NNNCATAAGTAATTTCATGA NGG 2 0.4444 Tier II
13 NC_000068.7 2 135377250 + Plcb1 NNNCAGAAGTAATTACAGGG NGG 2 0.4427 Tier II
14 NC_000069.6 3 154199525 + Slc44a5 NNNCAGAAGCAACTTCAGGA NGG 2 0.4211 Tier II
15 NC_000078.6 12 41200115 - Immp2l NNNCAGAAGGAATTTCAGTA NGG 2 0.3333 Tier II
16 NC_000075.6 9 95916933 - Atr NNNCAGAATGAATTTCAGGA NGG 2 0.2667 Tier II
17 NC_000070.6 4 120594038 + Gm8439 NNNCAGAAGTAATTTAAGGA NAG 2 0.2593 Tier II
18 NC_000072.6 6 73091232 - Dnah6 NNNCAGAAGTAATTTTAGAA NGG 2 0.2198 Tier II
19 NC_000086.7 X 139720180 + Tbc1d8b NNNCAGAAGTTAATTCAGGA NGG 2 0.213 Tier II
20 NC_000082.6 16 36597905 + Cd86 NNNCAGAAGTAATTTTATGA NGG 2 0.2051 Tier II
21 NC_000073.6 7 144115424 + Shank2 NNNCAGAAGTAAATCCAGGA NGG 2 0.1888 Tier II
22 NC_000067.6 1 17765615 + Crispld1 NNNCAGAAGTAATTTCAGAA NAG 2 0.1852 Tier II
23 NC_000075.6 9 70484482 + Rnf111 NNNCAGAATTAATTTCAGGA NAG 2 0.1383 Tier II
24 NC_000084.6 18 41996239 - Grxcr2 NNNCAGAAGTCTTTTCAGGA NGG 2 0.1333 Tier II
25 NC_000077.6 11 80887392 + Asic2 NNNCAGAAGTCATCTCAGGA NGG 2 0.1143 Tier II
26 NC_000072.6 6 85944708 + Dusp11 NNNCAGATGTAATTTCAGGA NAG 2 0.1111 Tier II
27 NC_000081.6 15 86404888 - Tbc1d22a NNNCTGAAGTAAGTTCAGGA NGG 2 0.0949 Tier II
28 NC_000075.6 9 72673227 - Nedd4 NNNCAGAAGTAATTTTAGGA NAG 2 0.0798 Tier II
29 NC_000068.7 2 109956339 + Lgr4 NNNGAGAAGTAATTTCTGGA NGG 2 0.0667 Tier II
30 NC_000077.6 11 50758084 + Adamts2 NNNCAGAAGCAATTTCAGGA NCG 2 0.0571 Tier II
31 NC_000067.6 1 46206912 - Dnah7b NNNCAGAAGTAATTTCAGTA NGA 2 0.0463 Tier II
32 NC_000067.6 1 46631571 - Dnah7c NNNCAGAAGTAATTTCAGTA NGA 2 0.0463 Tier II
33 NC_000072.6 6 46389041 - Cntnap2 NNNCAGAAGTAAGTTGAGGA NGG 2 0.0401 Tier II
34 NC_000071.6 5 9153233 + Dmtf1 NNNCAGAAGTAATTTCGGGC NGG 2 0.0401 Tier II
35 NC_000072.6 6 4904675 - Ppp1r9a NNNCAGAAGTAAGTTCTGGA NGG 2 0.0348 Tier II
36 NC_000072.6 6 8154828 - Col28a1 NNNAAGAAGTAATTTCAGGA NTG 2 0.0328 Tier II
37 NC_000072.6 6 127587569 + Cracr2a NNNCAGAAGTATTTTCAGGA NGA 2 0.0231 Tier II
38 NC_000067.6 1 24203868 + Col9a1 NNNCAGAAGTTATTTCAGGA NTG 2 0.012 Tier II
39 NC_000073.6 7 117262502 - Gm39075 NNNTAGAAGTAATTTAAGGA NGG 2 0.8 Tier III
40 NC_000068.7 2 169627904 + Gm34294 NNNCAGAAGCAACTTCAGGA NGG 2 0.4211 Tier III
41 NC_000067.6 1 153411089 - E330020D12Rik NNNCAGAAGTAAGTTCATGA NGG 2 0.1739 Tier III
42 NC_000070.6 4 10788325 + 1700123O12Rik NNNCAGAATTAATTTCTGGA NGG 2 0.0711 Tier III
43 NC_000077.6 11 50758084 + Gm51860 NNNCAGAAGCAATTTCAGGA NCG 2 0.0571 Tier III
44 NC_000080.6 14 53898462 + Tcra NNNCAGAGGTAATTTCAGGA NGA 2 0.0509 Tier III
45 NC_000080.6 14 53898462 + Gm30392 NNNCAGAGGTAATTTCAGGA NGA 2 0.0509 Tier III
46 NC_000071.6 5 129636804 + Gm18390 NNNCAGAAGTAATTTCTGGA NTG 2 0.0052 Tier III
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Other clones with same target sequence:

(none)