Construct: sgRNA BRDN0001147277

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGGATTGTATGTACCCGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKG1 (5592)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77006

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 51153259 + PRKG1 NNNGGATTGTATGTACCCGG NGG 0 1.0 Tier I
2 NC_000004.12 4 2171485 + POLN NNNGGATTGTATGAACCCGA NGG 2 0.5804 Tier II
3 NC_000016.10 16 81770639 + PLCG2 NNNGGATTGTATGAGCCCGG NGG 2 0.4024 Tier II
4 NC_000006.12 6 110833402 + AMD1 NNNTGATTGTATGTACCAGG NGG 2 0.1958 Tier II
5 NC_000016.10 16 81770639 + LOC105369213 NNNGGATTGTATGAGCCCGG NGG 2 0.4024 Tier III
6 NC_000003.12 3 158926286 - LOC124909452 NNNGGATTGTATGTACCTCG NGG 2 0.2882 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 31585639 - Prkg1 NNNGGACTGTATGTACCCCG NGG 2 0.3082 Tier I
2 NC_000079.6 13 24962702 + Gpld1 NNNGGATTGTATGTACACGG NGC 2 0.0104 Tier II
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Other clones with same target sequence:

(none)