Construct: sgRNA BRDN0001147290

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATGAGCAGGAGCAAACATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCLK3 (85443)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77313

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 36737845 - DCLK3 NNNAGCAGGAGCAAACATGG NGG 0 1.0 Tier I
2 NC_000019.10 19 32868534 - SLC7A9 NNNAGCAGGAGGAAACATGG NGG 1 0.4444 Tier I
3 NC_000020.11 20 16368028 + KIF16B NNNAGCAGGAGCAAACACGC NGG 2 0.1837 Tier I
4 NC_000004.12 4 102607194 - NFKB1 NNNAGCAGCAGCAAACATGG NAG 2 0.1396 Tier I
5 NC_000008.11 8 22617708 + CCAR2 NNNAGCAGGAGCAAAGCTGG NGG 2 0.0271 Tier I
6 NC_000023.11 X 75131620 - ABCB7 NNNAGCAGCAGCAAAAATGG NGG 2 0.5385 Tier II
7 NC_000020.11 20 35690381 - NFS1 NNNAGGAGGAGCAAAAATGG NGG 2 0.5 Tier II
8 NC_000012.12 12 102117819 + NUP37 NNNAGCAGGAGTAAACATGT NGG 2 0.3769 Tier II
9 NC_000008.11 8 144391514 + ADCK5 NNNAGCAGGAGCAAACACGT NGG 2 0.3 Tier II
10 NC_000011.10 11 111926484 - C11orf52 NNNAGCAGGAGCAACAATGG NGG 2 0.2727 Tier II
11 NC_000008.11 8 14133274 - SGCZ NNNATCAGGAGCAAACATTG NGG 2 0.2 Tier II
12 NC_000015.10 15 57387563 + CGNL1 NNNAGCAGGAGCACACATAG NGG 2 0.1531 Tier II
13 NC_000007.14 7 70452522 - AUTS2 NNNAGCAGGAGGAAACAGGG NGG 2 0.1481 Tier II
14 NC_000008.11 8 70660619 - LACTB2 NNNTGCAGGAGCAAAGATGG NGG 2 0.0979 Tier II
15 NC_000004.12 4 143521137 - SMARCA5 NNNAGCAGGAGCAAACTTTG NGG 2 0.0889 Tier II
16 NC_000011.10 11 46340606 - DGKZ NNNAGCAGGAGGAAACCTGG NGG 2 0.0784 Tier II
17 NC_000020.11 20 47001332 - EYA2 NNNAGCAGGAGCAGACTTGG NGG 2 0.0622 Tier II
18 NC_000017.11 17 79209301 - RBFOX3 NNNAGCAGGTGCAAACATGG NGA 2 0.0613 Tier II
19 NC_000001.11 1 3662087 - TP73 NNNAGCAGGAGGAAACATGG NTG 2 0.0173 Tier II
20 NC_000008.11 8 101718638 + NCALD NNNAGCAGGAGCTAACATGG NGC 2 0.0067 Tier II
21 NC_000008.11 8 133156991 + LOC105375769 NNNAGCAGAAGAAAACATGG NGG 2 0.4592 Tier III
22 NC_000002.12 2 58559068 - LINC01122 NNNAGCAGGAGGAAAAATGG NGG 2 0.4444 Tier III
23 NC_000001.11 1 235933627 - LINC02768 NNNAGCAGGGGCAAGCATGG NGG 2 0.3611 Tier III
24 NC_000001.11 1 235933627 - LOC105373215 NNNAGCAGGGGCAAGCATGG NGG 2 0.3611 Tier III
25 NC_000013.11 13 46200104 + LOC107984578 NNNAGAAGGCGCAAACATGG NGG 2 0.3095 Tier III
26 NC_000002.12 2 218915145 + LINC01494 NNNAGCAGGACAAAACATGG NGG 2 0.3061 Tier III
27 NC_000002.12 2 218915145 + LOC124906119 NNNAGCAGGACAAAACATGG NGG 2 0.3061 Tier III
28 NC_000006.12 6 8646775 - LOC100506207 NNNAGCAGGAACAACCATGG NGG 2 0.2727 Tier III
29 NC_000011.10 11 111926484 - HSPB2-C11orf52 NNNAGCAGGAGCAACAATGG NGG 2 0.2727 Tier III
30 NC_000009.12 9 1367466 + LOC102723803 NNNAGCAGGAGCAAGCAGGG NGG 2 0.2167 Tier III
31 NC_000006.12 6 169887275 + LOC105378149 NNNAGCAGGAGCACAAATGG NGG 2 0.2143 Tier III
32 NC_000011.10 11 87671819 - LOC107984361 NNNAGCAGGAACAAAGATGG NGG 2 0.1538 Tier III
33 NC_000004.12 4 102607194 - LOC105377347 NNNAGCAGCAGCAAACATGG NAG 2 0.1396 Tier III
34 NC_000012.12 12 102707000 - LINC02456 NNNAGCAGGAGCAAAGAAGG NGG 2 0.1026 Tier III
35 NC_000008.11 8 70660619 - LACTB2-AS1 NNNTGCAGGAGCAAAGATGG NGG 2 0.0979 Tier III
36 NC_000006.12 6 156251815 - LOC101928923 NNNAGCAGGAGGAAACTTGG NGG 2 0.0593 Tier III
37 NC_000016.10 16 52593020 - CASC16 NNNAGCAGCAGCAAACATGG NTG 2 0.021 Tier III
38 NC_000001.11 1 3662087 - TP73-AS3 NNNAGCAGGAGGAAACATGG NTG 2 0.0173 Tier III
39 NC_000002.12 2 52965920 - LOC105369165 NNNAGCAGGAGCAAACATGG NGT 1 0.0161 Tier III
40 NC_000013.11 13 90907329 + LINC00410 NNNAGCAGGAGCAAACTTGG NGC 2 0.003 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 82037146 - Xrcc6 NNNACCAAGAGCAAACATGG NGG 2 0.7857 Tier II
2 NC_000070.6 4 59333060 + Susd1 NNNAGAAGGAGAAAACATGG NGG 2 0.6633 Tier II
3 NC_000075.6 9 70255732 - Myo1e NNNAGAAGGAGAAAACATGG NGG 2 0.6633 Tier II
4 NC_000075.6 9 117112539 - Rbms3 NNNGGCAGGAGCAAAAATGG NGG 2 0.625 Tier II
5 NC_000072.6 6 50387279 + Osbpl3 NNNACCAGGAGAAAACATGG NGG 2 0.5612 Tier II
6 NC_000077.6 11 115323863 + Ush1g NNNAGGAAGAGCAAACATGG NGG 2 0.5 Tier II
7 NC_000077.6 11 115323863 + Otop2 NNNAGGAAGAGCAAACATGG NGG 2 0.5 Tier II
8 NC_000083.6 17 8950764 + Pde10a NNNAGCAGGAGAAAACAAGG NGG 2 0.4762 Tier II
9 NC_000071.6 5 29782370 + Dnajb6 NNNAGCAGAAGCAAACATAG NGG 2 0.4592 Tier II
10 NC_000084.6 18 80079572 + Pard6g NNNAGCAGGAGGAAACATGG NGG 1 0.4444 Tier II
11 NC_000071.6 5 105217684 - Gbp10 NNNAGCTGGAACAAACATGG NGG 2 0.4375 Tier II
12 NC_000071.6 5 105273482 - Gbp6 NNNAGCTGGAACAAACATGG NGG 2 0.4375 Tier II
13 NC_000086.7 X 150851971 + Itih5l-ps NNNAGAAGGAGCAGACATGG NGG 2 0.4333 Tier II
14 NC_000073.6 7 132087338 - Cpxm2 NNNAGCAGAAGCAAGCATGG NGG 2 0.4179 Tier II
15 NC_000077.6 11 32191645 - Il9r NNNGGCAGGAGCAAGCATGG NGG 2 0.4063 Tier II
16 NC_000075.6 9 60103397 - Thsd4 NNNTGCATGAGCAAACATGG NGG 2 0.3977 Tier II
17 NC_000068.7 2 84983134 - Prg2 NNNAGCAGAAGCATACATGG NGG 2 0.3429 Tier II
18 NC_000069.6 3 27607513 - Fndc3b NNNAGAAGGAGCAAACAGGG NGG 2 0.3095 Tier II
19 NC_000071.6 5 137037961 - Ap1s1 NNNAGCAGGAGCAGGCATGG NGG 2 0.3033 Tier II
20 NC_000073.6 7 143179480 + Kcnq1 NNNAGCAGGAGCAGGCATGG NGG 2 0.3033 Tier II
21 NC_000079.6 13 42983120 - Phactr1 NNNAGCAGAAGCAGACATGG NGG 2 0.3 Tier II
22 NC_000072.6 6 29443939 + Flnc NNNAGCAGAAGGAAACATGG NGG 2 0.2857 Tier II
23 NC_000070.6 4 114603576 + Trabd2b NNNAGCAGGAGCAAACATGG NAG 1 0.2593 Tier II
24 NC_000083.6 17 34904575 - Ehmt2 NNNAGCAGCAGCAGACATGG NGG 2 0.2513 Tier II
25 NC_000067.6 1 125928510 + Nckap5 NNNAGCTGGAGTAAACATGG NGG 2 0.2356 Tier II
26 NC_000067.6 1 162275461 + Dnm3 NNNAGCTGTAGCAAACATGG NGG 2 0.2333 Tier II
27 NC_000077.6 11 79648653 + Rab11fip4 NNNCGCAGGAGCAAGCATGG NGG 2 0.2294 Tier II
28 NC_000068.7 2 35956913 - Ttll11 NNNAGCAGGAGATAACATGG NGG 2 0.2143 Tier II
29 NC_000082.6 16 11576405 + Snx29 NNNAGCAGGAGAACACATGG NGG 2 0.1531 Tier II
30 NC_000076.6 10 89076492 - Ano4 NNNAGCAGGAGAAATCATGG NGG 2 0.1429 Tier II
31 NC_000080.6 14 56605542 + Parp4 NNNAGCAGGAGCTGACATGG NGG 2 0.14 Tier II
32 NC_000071.6 5 27477112 + Dpp6 NNNAGCATGAGCACACATGG NGG 2 0.1339 Tier II
33 NC_000068.7 2 27171711 + Dbh NNNAGCAGCAGCAATCATGG NGG 2 0.1077 Tier II
34 NC_000070.6 4 63032251 + Zfp618 NNNAGCAGGAGCAGAGATGG NGG 2 0.0718 Tier II
35 NC_000081.6 15 85977581 - Celsr1 NNNAGCAGGAGGAAAGATGG NGG 2 0.0684 Tier II
36 NC_000079.6 13 24460856 + Cmah NNNAGCAGGAGCAAGCATGG NTG 2 0.0253 Tier II
37 NC_000068.7 2 135180827 - Plcb1 NNNAGCAGGAGCAAATATGG NGA 2 0.0214 Tier II
38 NC_000069.6 3 59166842 + Med12l NNNAGCAGCAGCAAACATGG NTG 2 0.021 Tier II
39 NC_000076.6 10 59003033 - Sh3rf3 NNNAGCAGCAGCAAACATGG NTG 2 0.021 Tier II
40 NC_000079.6 13 8459701 + Adarb2 NNNAGCAGCAGCAAACATGG NTG 2 0.021 Tier II
41 NC_000083.6 17 50805154 - Tbc1d5 NNNAGCAGCAGCAAACATGG NTG 2 0.021 Tier II
42 NC_000084.6 18 12438216 + Lama3 NNNAGAAGGAGCAAACATGG NGT 2 0.015 Tier II
43 NC_000078.6 12 112994388 + Brf1 NNNAGCAGGAGGAAACATGG NGC 2 0.0099 Tier II
44 NC_000082.6 16 6435493 - Rbfox1 NNNAGCAGGAGCACACATGG NTG 2 0.0083 Tier II
45 NC_000084.6 18 31864061 + Wdr33 NNNAGGAGGAGCAAACATGG NGT 2 0.0081 Tier II
46 NC_000078.6 12 75140417 + Kcnh5 NNNAGCAGGAGGAAACATGG NGT 2 0.0072 Tier II
47 NC_000072.6 6 50387279 + Gm52900 NNNACCAGGAGAAAACATGG NGG 2 0.5612 Tier III
48 NC_000071.6 5 105217684 - Gm31785 NNNAGCTGGAACAAACATGG NGG 2 0.4375 Tier III
49 NC_000082.6 16 73216893 - 4930500H12Rik NNNAGCAGGAGGAAACAAGG NGG 2 0.2963 Tier III
50 NC_000078.6 12 79113916 - Gm8255 NNNAGCAGGAGCAACAATGG NGG 2 0.2727 Tier III
51 NC_000070.6 4 114603576 + Gm33655 NNNAGCAGGAGCAAACATGG NAG 1 0.2593 Tier III
52 NC_000078.6 12 22090961 + Gm9283 NNNAGCAGGAGAAATCATGG NGG 2 0.1429 Tier III
53 NC_000078.6 12 23249917 + Gm9291 NNNAGCAGGAGAAATCATGG NGG 2 0.1429 Tier III
54 NC_000079.6 13 57385459 - Gm33294 NNNATCTGGAGCAAACATGG NGG 2 0.1313 Tier III
55 NC_000073.6 7 133139348 + 4930483O08Rik NNNAGCAGGAGCAAAGATGC NGG 2 0.0659 Tier III
56 NC_000074.6 8 45236052 + Gm30504 NNNAGCAGGAGCAAACATGA NTG 2 0.0365 Tier III
57 NC_000068.7 2 135180827 - 4930545L23Rik NNNAGCAGGAGCAAATATGG NGA 2 0.0214 Tier III
58 NC_000070.6 4 54858039 + Gm34133 NNNAGCAGGAGCAGACATGG NTG 2 0.0182 Tier III
59 NC_000075.6 9 100311698 + Gm28167 NNNAGCAGGAGCTAACATGG NGC 2 0.0067 Tier III
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Other clones with same target sequence:

(none)