Construct: sgRNA BRDN0001147291
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGCTGTCGGTGATCCACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K3 (5606)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76379
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
21303230 |
+ |
MAP2K3 |
NNNCTGTCGGTGATCCACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
140756004 |
+ |
TBC1D9 |
NNNCTCTCGGTGATCCACAG |
NGC |
2 |
0.0152 |
Tier I |
| 3 |
NC_000001.11 |
1 |
226736659 |
+ |
ITPKB |
NNNCTGTGGGTGAGCCACAG |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000013.11 |
13 |
30527466 |
+ |
HMGB1 |
NNNATGTCGGTGGTCCACAG |
NGG |
2 |
0.5492 |
Tier II |
| 5 |
NC_000001.11 |
1 |
13968388 |
- |
KAZN |
NNNCTGTCGGGGATCCACAG |
NGG |
1 |
0.4 |
Tier II |
| 6 |
NC_000017.11 |
17 |
32153960 |
+ |
RHOT1 |
NNNCTGTCTGTGATCCACAG |
NGA |
2 |
0.037 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
60945558 |
+ |
Map2k3 |
NNNCTGTCTGTGATCCACAG |
NGG |
1 |
0.5333 |
Tier I |
| 2 |
NC_000074.6 |
8 |
33581895 |
- |
Tex15 |
NNNCTGTGTGTGATCCACAG |
NGG |
2 |
0.3429 |
Tier I |
| 3 |
NC_000068.7 |
2 |
181621947 |
- |
Prpf6 |
NNNCTGTCGGGGATCCACAG |
NAG |
2 |
0.1037 |
Tier I |
| 4 |
NC_000074.6 |
8 |
83166117 |
- |
Tbc1d9 |
NNNCTCTCGGTGATCCACAG |
NGC |
2 |
0.0152 |
Tier I |
| 5 |
NC_000084.6 |
18 |
84731334 |
+ |
Dipk1c |
NNNCTATCTGTGATCCACAG |
NGG |
2 |
0.5333 |
Tier II |
| 6 |
NC_000072.6 |
6 |
6154442 |
- |
Slc25a13 |
NNNCTGTCGGTGACCCACAG |
NGG |
1 |
0.2857 |
Tier II |
| 7 |
NC_000067.6 |
1 |
167770147 |
+ |
Lmx1a |
NNNCTGTGGGGGATCCACAG |
NGG |
2 |
0.2571 |
Tier II |
| 8 |
NC_000077.6 |
11 |
20647271 |
- |
Sertad2 |
NNNCTGTCGGGTATCCACAG |
NGG |
2 |
0.1538 |
Tier II |
| 9 |
NC_000082.6 |
16 |
20539253 |
+ |
Ap2m1 |
NNNCTGTAGGTGATCCTCAG |
NGG |
2 |
0.0867 |
Tier II |
| 10 |
NC_000084.6 |
18 |
54941268 |
- |
Zfp608 |
NNNCTGTCTGTGATTCACAG |
NGG |
2 |
0.0356 |
Tier II |
| 11 |
NC_000071.6 |
5 |
125091251 |
- |
Ncor2 |
NNNCTGTCGATGAGCCACAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000082.6 |
16 |
42793760 |
- |
4932412D23Rik |
NNNTTTTCGGTGATCCACAG |
NGG |
2 |
0.5333 |
Tier III |
Other clones with same target sequence:
(none)
