Construct: sgRNA BRDN0001147291

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGCTGTCGGTGATCCACAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K3 (5606)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76379

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 21303230 + MAP2K3 NNNCTGTCGGTGATCCACAG NGG 0 1.0 Tier I
2 NC_000004.12 4 140756004 + TBC1D9 NNNCTCTCGGTGATCCACAG NGC 2 0.0152 Tier I
3 NC_000001.11 1 226736659 + ITPKB NNNCTGTGGGTGAGCCACAG NGG 2 0.0 Tier I
4 NC_000013.11 13 30527466 + HMGB1 NNNATGTCGGTGGTCCACAG NGG 2 0.5492 Tier II
5 NC_000001.11 1 13968388 - KAZN NNNCTGTCGGGGATCCACAG NGG 1 0.4 Tier II
6 NC_000017.11 17 32153960 + RHOT1 NNNCTGTCTGTGATCCACAG NGA 2 0.037 Tier II
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 60945558 + Map2k3 NNNCTGTCTGTGATCCACAG NGG 1 0.5333 Tier I
2 NC_000074.6 8 33581895 - Tex15 NNNCTGTGTGTGATCCACAG NGG 2 0.3429 Tier I
3 NC_000068.7 2 181621947 - Prpf6 NNNCTGTCGGGGATCCACAG NAG 2 0.1037 Tier I
4 NC_000074.6 8 83166117 - Tbc1d9 NNNCTCTCGGTGATCCACAG NGC 2 0.0152 Tier I
5 NC_000084.6 18 84731334 + Dipk1c NNNCTATCTGTGATCCACAG NGG 2 0.5333 Tier II
6 NC_000072.6 6 6154442 - Slc25a13 NNNCTGTCGGTGACCCACAG NGG 1 0.2857 Tier II
7 NC_000067.6 1 167770147 + Lmx1a NNNCTGTGGGGGATCCACAG NGG 2 0.2571 Tier II
8 NC_000077.6 11 20647271 - Sertad2 NNNCTGTCGGGTATCCACAG NGG 2 0.1538 Tier II
9 NC_000082.6 16 20539253 + Ap2m1 NNNCTGTAGGTGATCCTCAG NGG 2 0.0867 Tier II
10 NC_000084.6 18 54941268 - Zfp608 NNNCTGTCTGTGATTCACAG NGG 2 0.0356 Tier II
11 NC_000071.6 5 125091251 - Ncor2 NNNCTGTCGATGAGCCACAG NGG 2 0.0 Tier II
12 NC_000082.6 16 42793760 - 4932412D23Rik NNNTTTTCGGTGATCCACAG NGG 2 0.5333 Tier III
Download CSV

Other clones with same target sequence:

(none)

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