Construct: sgRNA BRDN0001147294
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTGACCGACTGCCTTCTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK12 (51755)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75916
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
39462883 |
- |
CDK12 |
NNNGACCGACTGCCTTCTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
133532852 |
+ |
CYP2E1 |
NNNCACCGACTGCCTGCTCG |
NGG |
2 |
0.1833 |
Tier I |
| 3 |
NC_000011.10 |
11 |
26292895 |
- |
ANO3 |
NNNGACAGACTGCCTCCTCG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000019.10 |
19 |
454437 |
- |
SHC2 |
NNNGACCGACCGCCTTCCCG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000003.12 |
3 |
42664671 |
+ |
ZBTB47 |
NNNGACCCACTGCCTTCCCG |
NGG |
2 |
0.2637 |
Tier II |
| 6 |
NC_000018.10 |
18 |
79916996 |
+ |
SLC66A2 |
NNNGACCGACTGCCTGCTCG |
NTG |
2 |
0.0135 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
128694630 |
- |
Inpp5f |
NNNGACCGACTGCCTAATCG |
NGG |
2 |
0.4242 |
Tier I |
| 2 |
NC_000076.6 |
10 |
108856618 |
+ |
Syt1 |
NNNGACAGACTGCCTTCTTG |
NGG |
2 |
0.3462 |
Tier II |
| 3 |
NC_000068.7 |
2 |
9922585 |
- |
Taf3 |
NNNGACTGACTGGCTTCTCG |
NGG |
2 |
0.1108 |
Tier II |
| 4 |
NC_000078.6 |
12 |
106479781 |
- |
Gm32876 |
NNNGACCGACTGCATTCTCC |
NGG |
2 |
0.15 |
Tier III |
| 5 |
NC_000068.7 |
2 |
9922585 |
- |
Gm31290 |
NNNGACTGACTGGCTTCTCG |
NGG |
2 |
0.1108 |
Tier III |
Other clones with same target sequence:
(none)
