Construct: sgRNA BRDN0001147315

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAAAAATTGGTCAAAGATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAR2B (5577)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76151

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 107140896 + PRKAR2B NNNAAAATTGGTCAAAGATG NGG 0 1.0 Tier I
2 NC_000013.11 13 79512098 - NDFIP2 NNNAAAATTAGTCAAAAATG NGG 2 0.8711 Tier II
3 NC_000003.12 3 57234272 - APPL1 NNNAAAATTTGTCAAAGATA NGG 2 0.7617 Tier II
4 NC_000004.12 4 122919707 - NUDT6 NNNAAGACTGGTCAAAGATG NGG 2 0.7143 Tier II
5 NC_000009.12 9 93532951 + FAM120A NNNAAAGTTGGTCAAAGATG NGG 1 0.7059 Tier II
6 NC_000008.11 8 14954310 + SGCZ NNNAAAATTGATCAAAGATT NGG 2 0.7 Tier II
7 NC_000008.11 8 711172 - ERICH1 NNNAAAATGGGCCAAAGATG NGG 2 0.5865 Tier II
8 NC_000003.12 3 70198951 + MDFIC2 NNNAAAGATGGTCAAAGATG NGG 2 0.5647 Tier II
9 NC_000014.9 14 89184284 + FOXN3 NNNAAAGTTGGACAAAGATG NGG 2 0.5647 Tier II
10 NC_000009.12 9 72563004 + TMC1 NNNAAAATTGGACAGAGATG NGG 2 0.52 Tier II
11 NC_000010.11 10 54868548 - PCDH15 NNNAAAGGTGGTCAAAGATG NGG 2 0.5176 Tier II
12 NC_000006.12 6 73706664 + CD109 NNNGAAATTGGTCAAAGATT NGG 2 0.4375 Tier II
13 NC_000001.11 1 220778946 - MTARC2 NNNAGAATTGGGCAAAGATG NGG 2 0.36 Tier II
14 NC_000013.11 13 41579003 + VWA8 NNNAATTTTGGTCAAAGATG NGG 2 0.3125 Tier II
15 NC_000020.11 20 9785624 + PAK5 NNNAGAATTGCTCAAAGATG NGG 2 0.3086 Tier II
16 NC_000003.12 3 30771664 - GADL1 NNNAAAAATGTTCAAAGATG NGG 2 0.3077 Tier II
17 NC_000003.12 3 192306470 + FGF12 NNNAAAATTGGTCAAAGAAG NGG 1 0.2857 Tier II
18 NC_000005.10 5 136981564 - SPOCK1 NNNAAAATTGATCAAAGATG NAG 2 0.2593 Tier II
19 NC_000012.12 12 25501790 - LMNTD1 NNNAAAATTGTTCAGAGATG NGG 2 0.25 Tier II
20 NC_000014.9 14 60269099 - PPM1A NNNTAAATTGGTAAAAGATG NGG 2 0.2448 Tier II
21 NC_000003.12 3 119298059 - ARHGAP31 NNNAAAAGTGGTCAAAGAAG NGG 2 0.2095 Tier II
22 NC_000003.12 3 78603220 - ROBO1 NNNAAAAGTGGTCAAAGATG NAG 2 0.1901 Tier II
23 NC_000001.11 1 180276398 - ACBD6 NNNAAAATTGGTCAGAGAAG NGG 2 0.1857 Tier II
24 NC_000001.11 1 180276398 - LHX4 NNNAAAATTGGTCAGAGAAG NGG 2 0.1857 Tier II
25 NC_000009.12 9 9257518 + PTPRD NNNAAAATTGGCCAAGGATG NGG 2 0.1822 Tier II
26 NC_000011.10 11 88897931 - GRM5 NNNAAAGTTGGTCAAACATG NGG 2 0.1661 Tier II
27 NC_000012.12 12 23730183 + SOX5 NNNAAAATTGCTAAAAGATG NGG 2 0.1648 Tier II
28 NC_000002.12 2 85547472 + GGCX NNNAAAATTGGTCAGAGAGG NGG 2 0.1625 Tier II
29 NC_000023.11 X 13797828 + GPM6B NNNAAAATTGGACAAAGCTG NGG 2 0.1524 Tier II
30 NC_000021.9 21 29107371 + MAP3K7CL NNNAAAATTGGTCTAAGATG NAG 2 0.1383 Tier II
31 NC_000014.9 14 39272411 + MIA2 NNNAAAATAGGTGAAAGATG NGG 2 0.1266 Tier II
32 NC_000013.11 13 51852543 - TMEM272 NNNAAAATTGCTCAAAGATG NAG 2 0.1111 Tier II
33 NC_000023.11 X 103330569 - TCEAL7 NNNAAAATTGGAGAAAGATG NGG 2 0.1091 Tier II
34 NC_000008.11 8 99850476 + VPS13B NNNAAAATTGGTAACAGATG NGG 2 0.1049 Tier II
35 NC_000002.12 2 27517783 - GCKR NNNAAAATTGTTCAAACATG NGG 2 0.0905 Tier II
36 NC_000011.10 11 64134821 - MACROD1 NNNAAAATGGGTGAAAGATG NGG 2 0.0844 Tier II
37 NC_000003.12 3 6869269 - GRM7 NNNAAAATTGGTGAAAGGTG NGG 2 0.0545 Tier II
38 NC_000008.11 8 132239692 - KCNQ3 NNNAAAATTGGTCATATATG NGG 2 0.05 Tier II
39 NC_000014.9 14 99482803 + CCNK NNNAAAATTGGTCATAGCTG NGG 2 0.0381 Tier II
40 NC_000014.9 14 99482803 + SETD3 NNNAAAATTGGTCATAGCTG NGG 2 0.0381 Tier II
41 NC_000006.12 6 145919176 - SHPRH NNNAAAAATGGTCAAAGATG NGC 2 0.0178 Tier II
42 NC_000008.11 8 543439 - TDRP NNNAATATTGGTCAAAGATG NGC 2 0.0159 Tier II
43 NC_000014.9 14 69346391 + GALNT16 NNNAATATTGGTCAAAGATG NGT 2 0.0115 Tier II
44 NC_000005.10 5 128284510 - FBN2 NNNAAAATTGTTCAAAGATG NGT 2 0.0062 Tier II
45 NC_000004.12 4 181645088 + TENM3 NNNAAAATTGGTGAATGATG NGG 2 0.0 Tier II
46 NC_000003.12 3 182174495 + LOC105374243 NNNAAAATAGGCCAAAGATG NGG 2 0.8797 Tier III
47 NC_000004.12 4 62126298 - ADGRL3-AS1 NNNAAAAATAGTCAAAGATG NGG 2 0.7467 Tier III
48 NC_000003.12 3 110939505 + LOC151760 NNNAAAATAGGACAAAGATG NGG 2 0.7429 Tier III
49 NC_000014.9 14 30551639 - G2E3-AS1 NNNAATATAGGTCAAAGATG NGG 2 0.6633 Tier III
50 NC_000003.12 3 70198951 + SAMMSON NNNAAAGATGGTCAAAGATG NGG 2 0.5647 Tier III
51 NC_000009.12 9 72563004 + LOC101927191 NNNAAAATTGGACAGAGATG NGG 2 0.52 Tier III
52 NC_000005.10 5 90182635 + LINC01339 NNNATAATTGGTCAAAGATG NGG 1 0.3636 Tier III
53 NC_000001.11 1 199066419 + LINC01221 NNNAAAATTGGTTGAAGATG NGG 2 0.3267 Tier III
54 NC_000013.11 13 32848305 - LINC00423 NNNAAAAATGGTCAAAGACG NGG 2 0.2207 Tier III
55 NC_000011.10 11 58968106 - LOC283194 NNNAAAGTTGGTCAAAGAAG NGG 2 0.2017 Tier III
56 NC_000011.10 11 58968106 - LOC100422398 NNNAAAGTTGGTCAAAGAAG NGG 2 0.2017 Tier III
57 NC_000011.10 11 49725200 + GRM5P1 NNNAAAGTTGGTCAAACATG NGG 2 0.1661 Tier III
58 NC_000002.12 2 191179388 - LOC105373804 NNNAATATTGGTCAAGGATG NGG 2 0.1374 Tier III
59 NC_000013.11 13 85109228 + LINC00375 NNNAAAATTGGTGAAAGGTG NGG 2 0.0545 Tier III
60 NC_000001.11 1 190780528 - LINC01720 NNNAAAATTAGTCAAAGATG NTG 2 0.0364 Tier III
61 NC_000020.11 20 52849756 + LOC105372668 NNNAAAATTGGTGAAAGAGG NGG 2 0.0341 Tier III
62 NC_000015.10 15 96213473 + NR2F2-AS1 NNNAAAATTTGTCAATGATG NGG 2 0.0 Tier III
63 NC_000015.10 15 96213473 + LOC112268156 NNNAAAATTTGTCAATGATG NGG 2 0.0 Tier III
64 NC_000003.12 3 181077202 + SOX2-OT NNNGAAATTGGTCAATGATG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 113955737 + Ssh1 NNNAAAGATGGTCAAAGATG NGG 2 0.5647 Tier I
2 NC_000078.6 12 31975977 - Prkar2b NNNAAAATTGGTCAAAGAAG NGG 1 0.2857 Tier I
3 NC_000078.6 12 78201821 + Gm6657 NNNAAAATGGGTCAAATATG NGG 2 0.1548 Tier I
4 NC_000085.6 19 22808185 + Trpm3 NNNAAAAATAGTCAAAGATG NGG 2 0.7467 Tier II
5 NC_000069.6 3 158530787 + Lrrc7 NNNAAGATTTGTCAAAGATG NGG 2 0.5804 Tier II
6 NC_000074.6 8 18989878 - Defb37 NNNAAAATGAGTCAAAGATG NGG 2 0.5778 Tier II
7 NC_000075.6 9 29186789 - Ntm NNNGAAATTTGTCAAAGATG NGG 2 0.5078 Tier II
8 NC_000072.6 6 106565574 - Cntn4 NNNAGAATTGGTCAGAGATG NGG 2 0.468 Tier II
9 NC_000067.6 1 175655044 - Kmo NNNGAAATGGGTCAAAGATG NGG 2 0.3869 Tier II
10 NC_000086.7 X 71443743 + Cd99l2 NNNAACATTTGTCAAAGATG NGG 2 0.3693 Tier II
11 NC_000081.6 15 57427852 - Slc22a22 NNNAAAATAGTTCAAAGATG NGG 2 0.3571 Tier II
12 NC_000083.6 17 64665766 + Man2a1 NNNAAATTTTGTCAAAGATG NGG 2 0.3555 Tier II
13 NC_000067.6 1 25661581 + Adgrb3 NNNAAAAATGGTCAAAGGTG NGG 2 0.32 Tier II
14 NC_000077.6 11 58158013 - Gemin5 NNNAAAATTTTTCAAAGATG NGG 2 0.3125 Tier II
15 NC_000074.6 8 84099449 - Dcaf15 NNNAAAAATGTTCAAAGATG NGG 2 0.3077 Tier II
16 NC_000086.7 X 72433720 - Gabra3 NNNAGAATTGTTCAAAGATG NGG 2 0.2769 Tier II
17 NC_000079.6 13 58899047 + Ntrk2 NNNAAAACTGGTCAAAGATG NAG 2 0.2593 Tier II
18 NC_000068.7 2 140875569 - Macrod2 NNNAAAATTGGCCACAGATG NGG 2 0.2584 Tier II
19 NC_000086.7 X 166459280 - Rab9 NNNATAATTGGTTAAAGATG NGG 2 0.2545 Tier II
20 NC_000069.6 3 132157721 - Dkk2 NNNAAAATTGGTCAAAGATA NAG 2 0.2431 Tier II
21 NC_000079.6 13 57845789 + Spock1 NNNAAAAATGGTCAAAGATG NAG 2 0.2074 Tier II
22 NC_000078.6 12 29745505 - Myt1l NNNAAAATTGGACAAAGATG NAG 2 0.2074 Tier II
23 NC_000079.6 13 62052228 - Gm3325 NNNAAAATTGGCCAAAGCTG NGG 2 0.1805 Tier II
24 NC_000077.6 11 58144470 - Gemin5 NNNAAAATTGCTCACAGATG NGG 2 0.1169 Tier II
25 NC_000067.6 1 64864729 + Plekhm3 NNNAAAATGGGTGAAAGATG NGG 2 0.0844 Tier II
26 NC_000075.6 9 95708523 + Trpc1 NNNAGAATTGGTCAAAGATG NGA 2 0.05 Tier II
27 NC_000067.6 1 97859598 + Pam NNNAAAATTGGTCAAAGATT NGA 2 0.0486 Tier II
28 NC_000068.7 2 97043686 + Lrrc4c NNNAAAATTGTTCAAAGATG NTG 2 0.015 Tier II
29 NC_000078.6 12 72256621 - Rtn1 NNNAAGATTGGTCAAAGATG NGT 2 0.0115 Tier II
30 NC_000074.6 8 18989878 - Gm39138 NNNAAAATGAGTCAAAGATG NGG 2 0.5778 Tier III
31 NC_000081.6 15 79168266 + Gm10863 NNNAATAGTGGTCAAAGATG NGG 2 0.5238 Tier III
32 NC_000084.6 18 68633978 + Gm30821 NNNAAAATGGGACAAAGATG NGG 2 0.4952 Tier III
33 NC_000070.6 4 84073053 - Gm33410 NNNAAAATTGGGCAAAGATT NGG 2 0.35 Tier III
34 NC_000070.6 4 84073053 - Gm33480 NNNAAAATTGGGCAAAGATT NGG 2 0.35 Tier III
35 NC_000074.6 8 84099449 - Gm39210 NNNAAAAATGTTCAAAGATG NGG 2 0.3077 Tier III
36 NC_000068.7 2 172334212 + Gm14273 NNNAAAATAGGTCAAAGAAG NGG 2 0.2653 Tier III
37 NC_000074.6 8 121202306 - 5033426O07Rik NNNACAATTGGTCAAAGATC NGG 2 0.2143 Tier III
38 NC_000079.6 13 62237178 - 2810408B13Rik NNNAAAATTGGCCAAAGCTG NGG 2 0.1805 Tier III
39 NC_000082.6 16 19225868 - Igl NNNATAATTGGTCAAAGAAG NGG 2 0.1039 Tier III
40 NC_000082.6 16 19225868 - Gm35545 NNNATAATTGGTCAAAGAAG NGG 2 0.1039 Tier III
41 NC_000075.6 9 95708523 + Gm39407 NNNAGAATTGGTCAAAGATG NGA 2 0.05 Tier III
42 NC_000069.6 3 54600807 - Gm40050 NNNAACATTGGTCAAAGATG NTG 2 0.0177 Tier III
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Other clones with same target sequence:

(none)