Construct: sgRNA BRDN0001147352

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCCAAGATAGCTCTCCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KC1 (26750)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75978

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 213240962 + RPS6KC1 NNNAAGATAGCTCTCCAAAG NGG 0 1.0 Tier I
2 NC_000011.10 11 85960716 + PICALM NNNAAGAGAGCTCTGCAAAG NGG 2 0.0367 Tier I
3 NC_000010.11 10 59223357 - PHYHIPL NNNAAGATAGCACTCAAAAG NGG 2 0.8 Tier II
4 NC_000016.10 16 47113178 + NETO2 NNNGAAATAGCTCTCCAAAG NGG 2 0.625 Tier II
5 NC_000012.12 12 93598926 + SOCS2 NNNAAAATTGCTCTCCAAAG NGG 2 0.6 Tier II
6 NC_000019.10 19 10153097 - DNMT1 NNNAAGATTGCTCTCCAAAA NGG 2 0.5625 Tier II
7 NC_000014.9 14 24086586 + NRL NNNAATGTAGCTCTCCAAAG NGG 2 0.4706 Tier II
8 NC_000017.11 17 17777232 + RAI1 NNNCAGAAAGCTCTCCAAAG NGG 2 0.2824 Tier II
9 NC_000007.14 7 114305501 - FOXP2 NNNAAGATAGCTCTCAAAAG NAG 2 0.2593 Tier II
10 NC_000006.12 6 7152903 + RREB1 NNNAAGATAGCCCTCCAAAG NAG 2 0.2456 Tier II
11 NC_000012.12 12 129647536 + TMEM132D NNNCAGATTGCTCTCCAAAG NGG 2 0.2118 Tier II
12 NC_000018.10 18 35620942 + GALNT1 NNNCAGATTGCTCTCCAAAG NGG 2 0.2118 Tier II
13 NC_000020.11 20 46653055 - SLC13A3 NNNAAGATAGTTCACCAAAG NGG 2 0.1905 Tier II
14 NC_000018.10 18 61889268 - RNF152 NNNAAGATAGCTCTCCAAAT NAG 2 0.1815 Tier II
15 NC_000006.12 6 148254559 + SASH1 NNNTAGATAGCTCTCCAAAG NAG 2 0.165 Tier II
16 NC_000004.12 4 153709017 - TLR2 NNNAAGAAAGCTCTCCACAG NGG 2 0.1524 Tier II
17 NC_000019.10 19 51356186 - ETFB NNNAAGAAAGCTCTCCACAG NGG 2 0.1524 Tier II
18 NC_000013.11 13 77065663 - MYCBP2 NNNAAGAGAGCTCTCGAAAG NGG 2 0.1128 Tier II
19 NC_000015.10 15 57488203 - CGNL1 NNNCAGATAGCTCTCCAAAG NAG 2 0.0915 Tier II
20 NC_000001.11 1 158010865 + KIRREL1 NNNCAGATAGCTCTCCCAAG NGG 2 0.0623 Tier II
21 NC_000003.12 3 123577258 - HACD2 NNNAAGATTGCTCTGCAAAG NGG 2 0.03 Tier II
22 NC_000005.10 5 62420965 + IPO11 NNNTAGATAGCTCTCCAAAG NTG 2 0.0248 Tier II
23 NC_000016.10 16 5034981 + NAGPA-AS1 NNNAAGAAAGCTCTCCAAAG NGG 1 0.8 Tier III
24 NC_000012.12 12 74049502 - LINC02394 NNNAAAATTGCTCTCCAAAG NGG 2 0.6 Tier III
25 NC_000002.12 2 46441588 + LINC02583 NNNAACCTAGCTCTCCAAAG NGG 2 0.2983 Tier III
26 NC_000005.10 5 159456558 - LINC01845 NNNCAGAAAGCTCTCCAAAG NGG 2 0.2824 Tier III
27 NC_000017.11 17 17777232 + SMCR5 NNNCAGAAAGCTCTCCAAAG NGG 2 0.2824 Tier III
28 NC_000017.11 17 17777232 + LOC124903942 NNNCAGAAAGCTCTCCAAAG NGG 2 0.2824 Tier III
29 NC_000004.12 4 55387313 - LINC02928 NNNAAGAAAGCTCTCCACAG NGG 2 0.1524 Tier III
30 NC_000011.10 11 129779309 + LOC124902793 NNNAAGAAAGCTCTCCACAG NGG 2 0.1524 Tier III
31 NC_000013.11 13 41150107 + KBTBD6-DT NNNAAGAAAGCTCTCCACAG NGG 2 0.1524 Tier III
32 NC_000024.10 Y 18884791 + TTTY14 NNNAAGAAAGCTCTCCACAG NGG 2 0.1524 Tier III
33 NC_000020.11 20 16183914 - LOC124904874 NNNAAGATAGCTCTCAAAAG NTG 2 0.039 Tier III
34 NC_000001.11 1 38195777 + LOC105378654 NNNAAGATAGCTCTCCAAAG NCT 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 76118764 - Nufip1 NNNAAAATAGCTCTCCAAAA NGG 2 0.9375 Tier II
2 NC_000078.6 12 24839664 - Mboat2 NNNAAGACAGCTCTCCAAAA NGG 2 0.9375 Tier II
3 NC_000068.7 2 102179134 + Ldlrad3 NNNAAGATATCTCTCAAAAG NGG 2 0.8125 Tier II
4 NC_000077.6 11 84524871 - Lhx1 NNNAAGAAAACTCTCCAAAG NGG 2 0.7467 Tier II
5 NC_000083.6 17 57927503 + Cntnap5c NNNAAAATAGCTCTCCAAAT NGG 2 0.7 Tier II
6 NC_000071.6 5 131537098 + Auts2 NNNAAGGTAGCTCTCCAAAA NGG 2 0.6618 Tier II
7 NC_000072.6 6 118639627 + Cacna1c NNNGAGAAAGCTCTCCAAAG NGG 2 0.5 Tier II
8 NC_000077.6 11 79365175 - Nf1 NNNAAGATTGATCTCCAAAG NGG 2 0.45 Tier II
9 NC_000070.6 4 146741877 + Zfp993 NNNATGATAGCTCTCCAAAA NGG 2 0.3409 Tier II
10 NC_000067.6 1 189742007 + Ptpn14 NNNAAGATTGCTCTCCAGAG NGG 2 0.24 Tier II
11 NC_000085.6 19 6259075 + Atg2a NNNAAGAAAGCTCCCCAAAG NGG 2 0.2286 Tier II
12 NC_000075.6 9 104698774 - Cpne4 NNNAAGAGAGCTCTCTAAAG NGG 2 0.2256 Tier II
13 NC_000074.6 8 68376357 + Csgalnact1 NNNAAGATAACTCTCCTAAG NGG 2 0.1244 Tier II
14 NC_000078.6 12 79373368 - Rad51b NNNAAGATAGCTCTTCAAAG NGG 1 0.0667 Tier II
15 NC_000081.6 15 92611968 - Pdzrn4 NNNATGATAGCTCTCCGAAG NGG 2 0.0642 Tier II
16 NC_000079.6 13 74766893 + Cast NNNAAGATAGCTCTCTGAAG NGG 2 0.0543 Tier II
17 NC_000068.7 2 38498614 - Nek6 NNNAAAATAGCTCTGCAAAG NGG 2 0.05 Tier II
18 NC_000084.6 18 42071963 + Sh3rf2 NNNAAGATAGCTCTCCAAAA NTG 2 0.0365 Tier II
19 NC_000079.6 13 81282688 + Adgrv1 NNNAAGATATCTCTCCAAAG NTG 2 0.0317 Tier II
20 NC_000069.6 3 118624564 + Dpyd NNNAAGAAAGCTCTCCAAAG NTG 2 0.0312 Tier II
21 NC_000075.6 9 66243950 + Dapk2 NNNAAGATAGCTCTACAAAG NGT 2 0.0036 Tier II
22 NC_000072.6 6 118598547 - Cacna1c NNNAAGATAGCTCTCCAAAG NCA 2 0.0 Tier II
23 NC_000074.6 8 110257777 + Gm39249 NNNAAGACAGATCTCCAAAG NGG 2 0.75 Tier III
24 NC_000072.6 6 22549385 + Gm38774 NNNAAGATAACTCTCCAAAT NGG 2 0.6533 Tier III
25 NC_000080.6 14 118092232 + Gm31633 NNNAAGATACCTCTCCAAAA NGG 2 0.375 Tier III
26 NC_000072.6 6 43022972 + Gm34905 NNNATGATAGCTCTCCAAAA NGG 2 0.3409 Tier III
27 NC_000072.6 6 58547803 + Gm45943 NNNATGATAGCTCTCCAAAA NGG 2 0.3409 Tier III
28 NC_000067.6 1 92829927 - Gm29481 NNNAAGATAGTGCTCCAAAG NGG 2 0.1538 Tier III
29 NC_000073.6 7 132384363 + Gm34908 NNNAAGATAGCTTTCCTAAG NGG 2 0.0933 Tier III
30 NC_000068.7 2 38498614 - Gm39799 NNNAAAATAGCTCTGCAAAG NGG 2 0.05 Tier III
31 NC_000072.6 6 118598547 - Gm38887 NNNAAGATAGCTCTCCAAAG NCA 2 0.0 Tier III
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Other clones with same target sequence:

(none)