Construct: sgRNA BRDN0001147402
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTATAGGTGAATACTCGGGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K5 (5607)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77264
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
67586857 |
+ |
MAP2K5 |
NNNTAGGTGAATACTCGGGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
79391401 |
+ |
RBFOX3 |
NNNTTGGTGAACACTCGGGC |
NGG |
2 |
0.3445 |
Tier II |
| 3 |
NC_000010.11 |
10 |
60456959 |
- |
ANK3 |
NNNTAGTTGAAGACTCGGGC |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000008.11 |
8 |
109319810 |
- |
NUDCD1 |
NNNTAGGTGAATATTGGGGC |
NGG |
2 |
0.1128 |
Tier II |
| 5 |
NC_000011.10 |
11 |
128566261 |
- |
ETS1 |
NNNTAGGTGGATACTGGGGC |
NGG |
2 |
0.0855 |
Tier II |
| 6 |
NC_000002.12 |
2 |
172542585 |
- |
PDK1-AS1 |
NNNTAGGTGAATAATCAGGC |
NGG |
2 |
0.3267 |
Tier III |
| 7 |
NC_000023.11 |
X |
103434656 |
+ |
LL0XNC01-250H12.3 |
NNNTAGGTGAATAATCAGGC |
NGG |
2 |
0.3267 |
Tier III |
| 8 |
NC_000011.10 |
11 |
128566261 |
- |
LOC105369565 |
NNNTAGGTGGATACTGGGGC |
NGG |
2 |
0.0855 |
Tier III |
| 9 |
NC_000004.12 |
4 |
6216799 |
- |
JAKMIP1-DT |
NNNTAGGTGAATACGCTGGC |
NGG |
2 |
0.0125 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
63338142 |
- |
Map2k5 |
NNNTAGGTGAATACACGGGC |
NGG |
1 |
0.5789 |
Tier I |
| 2 |
NC_000077.6 |
11 |
47865562 |
+ |
Sgcd |
NNNTAGGGGAATACTAGGGC |
NGG |
2 |
0.7333 |
Tier II |
| 3 |
NC_000079.6 |
13 |
76960737 |
+ |
Mctp1 |
NNNTAGGGGAATACTAGGGC |
NGG |
2 |
0.7333 |
Tier II |
| 4 |
NC_000080.6 |
14 |
93092499 |
+ |
Pcdh9 |
NNNTAGGTGAATGCTAGGGC |
NGG |
2 |
0.6522 |
Tier II |
| 5 |
NC_000086.7 |
X |
104336032 |
- |
Abcb7 |
NNNTAGGAGAATGCTCGGGC |
NGG |
2 |
0.5217 |
Tier II |
| 6 |
NC_000070.6 |
4 |
3714666 |
+ |
Lyn |
NNNTAGGTAAATTCTCGGGC |
NGG |
2 |
0.1929 |
Tier II |
| 7 |
NC_000076.6 |
10 |
83605993 |
+ |
Appl2 |
NNNTAGGTGAAGACTTGGGC |
NGG |
2 |
0.1538 |
Tier II |
| 8 |
NC_000083.6 |
17 |
75231324 |
- |
Ltbp1 |
NNNTAGGTGAATACTAGGGC |
NGT |
2 |
0.0161 |
Tier II |
Other clones with same target sequence:
(none)
