Construct: sgRNA BRDN0001147407

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCATAGATATTAACAACATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNNI3K (51086)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77898

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 74342952 - TNNI3K NNNTAGATATTAACAACATG NGG 0 1.0 Tier I
2 NC_000001.11 1 74342952 - FPGT-TNNI3K NNNTAGATATTAACAACATG NGG 0 1.0 Tier I
3 NC_000012.12 12 32720686 + DNM1L NNNTAGATATTAACAACAAG NAG 2 0.0741 Tier I
4 NC_000006.12 6 41232988 + TREML4 NNNTAGACAATAACAACATG NGG 2 0.8571 Tier II
5 NC_000008.11 8 118139102 + SAMD12 NNNTGGATATTAACAACATG NGG 1 0.72 Tier II
6 NC_000012.12 12 4544327 - RAD51AP1 NNNTAAATCTTAACAACATG NGG 2 0.5714 Tier II
7 NC_000008.11 8 3951728 + CSMD1 NNNTGGGTATTAACAACATG NGG 2 0.5082 Tier II
8 NC_000011.10 11 73738021 - RAB6A NNNTATATATTAATAACATG NGG 2 0.4889 Tier II
9 NC_000012.12 12 118306906 + TAOK3 NNNTAGCCATTAACAACATG NGG 2 0.4375 Tier II
10 NC_000013.11 13 92672185 - GPC5 NNNTAGAGATTAACAAAATG NGG 2 0.3422 Tier II
11 NC_000018.10 18 52483969 - DCC NNNTAGATATTAAAAACATA NGG 2 0.3281 Tier II
12 NC_000009.12 9 33268144 + CHMP5 NNNTAGCTATTAGCAACATG NGG 2 0.2853 Tier II
13 NC_000023.11 X 120377451 + ATP1B4 NNNTAAATATTCACAACATG NGG 2 0.2632 Tier II
14 NC_000009.12 9 14465482 + NFIB NNNTAGATATTAACAACATA NAG 2 0.2431 Tier II
15 NC_000014.9 14 52902452 + FERMT2 NNNTAGATATTAACAACAGA NGG 2 0.2344 Tier II
16 NC_000006.12 6 138679790 + NHSL1 NNNTAGATATCTACAACATG NGG 2 0.2222 Tier II
17 NC_000016.10 16 61889157 - CDH8 NNNCAGATATTTACAACATG NGG 2 0.2157 Tier II
18 NC_000007.14 7 23135254 - KLHL7 NNNTAGATATTAATAACAAG NGG 2 0.2095 Tier II
19 NC_000008.11 8 51750743 - PXDNL NNNTAGATATTAACAATATC NGG 2 0.2 Tier II
20 NC_000006.12 6 75913495 - MYO6 NNNTAGATATTAACAACATT NAG 2 0.1815 Tier II
21 NC_000022.11 22 33556380 + LARGE1 NNNTAGATATTAGCAACAGG NGG 2 0.163 Tier II
22 NC_000015.10 15 51575675 + DMXL2 NNNTAGATATTAACAACTTG NAG 2 0.1296 Tier II
23 NC_000007.14 7 107784150 - SLC26A3 NNNTAGATATTCACAAAATG NGG 2 0.1228 Tier II
24 NC_000023.11 X 38185020 - SRPX NNNTAGCTATTAACCACATG NGG 2 0.1193 Tier II
25 NC_000009.12 9 122745106 - OR1L6 NNNTAGATATTAGCAACATG NGA 2 0.0453 Tier II
26 NC_000012.12 12 39414855 + KIF21A NNNTAGATATTAATAACATG NTG 2 0.0286 Tier II
27 NC_000005.10 5 126621873 + PHAX NNNAAGATATTAACAACATG NTG 2 0.0186 Tier II
28 NC_000003.12 3 169508471 - MECOM NNNTAGATATTAACATCCTG NGG 2 0.0 Tier II
29 NC_000004.12 4 61532978 + ADGRL3 NNNTAGATATTAAGAACATG NGA 2 0.0 Tier II
30 NC_000007.14 7 14819776 - DGKB NNNTAGATATTAAGAACATG NGC 2 0.0 Tier II
31 NC_000002.12 2 114891351 - DPP10 NNNTAGATATTAAGTACATG NGG 2 0.0 Tier II
32 NC_000001.11 1 194253974 + LOC107985242 NNNTAAATATTAACAATATG NGG 2 0.4667 Tier III
33 NC_000013.11 13 92672185 - LOC105370315 NNNTAGAGATTAACAAAATG NGG 2 0.3422 Tier III
34 NC_000014.9 14 52902452 + LOC105370500 NNNTAGATATTAACAACAGA NGG 2 0.2344 Tier III
35 NC_000003.12 3 30380198 - LOC101927995 NNNTAGATATTAATAACAAG NGG 2 0.2095 Tier III
36 NC_000009.12 9 99688267 - LOC101928438 NNNTAGATACTAAAAACATG NGG 2 0.1867 Tier III
37 NC_000009.12 9 99688267 - LOC124902234 NNNTAGATACTAAAAACATG NGG 2 0.1867 Tier III
38 NC_000011.10 11 91847604 + LINC02756 NNNTAGATATGAACAACCTG NGG 2 0.0762 Tier III
39 NC_000016.10 16 63341688 + LOC105371308 NNNTAGATATTAACAACAAG NAG 2 0.0741 Tier III
40 NC_000016.10 16 61434631 + LOC105371302 NNNTTGATATTAACTACATG NGG 2 0.0727 Tier III
41 NC_000016.10 16 80360627 - DYNLRB2-AS1 NNNTAGAGATTAACAACATG NGT 2 0.0118 Tier III
42 NC_000013.11 13 110124182 + LINC03082 NNNTAGATATTAAAAACATG NGC 2 0.0078 Tier III
43 NC_000013.11 13 110124182 + LINC03032 NNNTAGATATTAAAAACATG NGC 2 0.0078 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 67106511 + St3gal1 NNNTAGATGATAACAACATG NGG 2 0.5714 Tier II
2 NC_000067.6 1 37655953 + Cracdl NNNTAGATTTTAATAACATG NGG 2 0.44 Tier II
3 NC_000067.6 1 177824827 - Catspere1 NNNTAGATAATAACAACATG NAG 2 0.2222 Tier II
4 NC_000076.6 10 12645282 + Utrn NNNTAGATATTCACAACATG NGC 2 0.0058 Tier II
5 NC_000069.6 3 18194245 + Cyp7b1 NNNTAGATATTAACAAGATG NGT 2 0.0009 Tier II
6 NC_000077.6 11 34730839 - Dock2 NNNTAGATATTAACACCATG NGG 1 0.0 Tier II
7 NC_000070.6 4 46760564 - Gabbr2 NNNTGGATATTAACACCATG NGG 2 0.0 Tier II
8 NC_000072.6 6 17163188 - Gm4876 NNNTACATATTAATAACATG NGG 2 0.5 Tier III
9 NC_000067.6 1 37655953 + 4930470B04Rik NNNTAGATTTTAATAACATG NGG 2 0.44 Tier III
10 NC_000076.6 10 84081841 - Gm34184 NNNTAGATTTTAACCACATG NGG 2 0.1636 Tier III
11 NC_000081.6 15 62669953 + Gm41333 NNNTAGATATTACCATCATG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)