Construct: sgRNA BRDN0001147407
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATAGATATTAACAACATG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- TNNI3K (51086)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000001.11 | 1 | 74342952 | - | TNNI3K | NNNTAGATATTAACAACATG | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 74342952 | - | FPGT-TNNI3K | NNNTAGATATTAACAACATG | NGG | 0 | 1.0 | Tier I |
3 | NC_000012.12 | 12 | 32720686 | + | DNM1L | NNNTAGATATTAACAACAAG | NAG | 2 | 0.0741 | Tier I |
4 | NC_000006.12 | 6 | 41232988 | + | TREML4 | NNNTAGACAATAACAACATG | NGG | 2 | 0.8571 | Tier II |
5 | NC_000008.11 | 8 | 118139102 | + | SAMD12 | NNNTGGATATTAACAACATG | NGG | 1 | 0.72 | Tier II |
6 | NC_000012.12 | 12 | 4544327 | - | RAD51AP1 | NNNTAAATCTTAACAACATG | NGG | 2 | 0.5714 | Tier II |
7 | NC_000008.11 | 8 | 3951728 | + | CSMD1 | NNNTGGGTATTAACAACATG | NGG | 2 | 0.5082 | Tier II |
8 | NC_000011.10 | 11 | 73738021 | - | RAB6A | NNNTATATATTAATAACATG | NGG | 2 | 0.4889 | Tier II |
9 | NC_000012.12 | 12 | 118306906 | + | TAOK3 | NNNTAGCCATTAACAACATG | NGG | 2 | 0.4375 | Tier II |
10 | NC_000013.11 | 13 | 92672185 | - | GPC5 | NNNTAGAGATTAACAAAATG | NGG | 2 | 0.3422 | Tier II |
11 | NC_000018.10 | 18 | 52483969 | - | DCC | NNNTAGATATTAAAAACATA | NGG | 2 | 0.3281 | Tier II |
12 | NC_000009.12 | 9 | 33268144 | + | CHMP5 | NNNTAGCTATTAGCAACATG | NGG | 2 | 0.2853 | Tier II |
13 | NC_000023.11 | X | 120377451 | + | ATP1B4 | NNNTAAATATTCACAACATG | NGG | 2 | 0.2632 | Tier II |
14 | NC_000009.12 | 9 | 14465482 | + | NFIB | NNNTAGATATTAACAACATA | NAG | 2 | 0.2431 | Tier II |
15 | NC_000014.9 | 14 | 52902452 | + | FERMT2 | NNNTAGATATTAACAACAGA | NGG | 2 | 0.2344 | Tier II |
16 | NC_000006.12 | 6 | 138679790 | + | NHSL1 | NNNTAGATATCTACAACATG | NGG | 2 | 0.2222 | Tier II |
17 | NC_000016.10 | 16 | 61889157 | - | CDH8 | NNNCAGATATTTACAACATG | NGG | 2 | 0.2157 | Tier II |
18 | NC_000007.14 | 7 | 23135254 | - | KLHL7 | NNNTAGATATTAATAACAAG | NGG | 2 | 0.2095 | Tier II |
19 | NC_000008.11 | 8 | 51750743 | - | PXDNL | NNNTAGATATTAACAATATC | NGG | 2 | 0.2 | Tier II |
20 | NC_000006.12 | 6 | 75913495 | - | MYO6 | NNNTAGATATTAACAACATT | NAG | 2 | 0.1815 | Tier II |
21 | NC_000022.11 | 22 | 33556380 | + | LARGE1 | NNNTAGATATTAGCAACAGG | NGG | 2 | 0.163 | Tier II |
22 | NC_000015.10 | 15 | 51575675 | + | DMXL2 | NNNTAGATATTAACAACTTG | NAG | 2 | 0.1296 | Tier II |
23 | NC_000007.14 | 7 | 107784150 | - | SLC26A3 | NNNTAGATATTCACAAAATG | NGG | 2 | 0.1228 | Tier II |
24 | NC_000023.11 | X | 38185020 | - | SRPX | NNNTAGCTATTAACCACATG | NGG | 2 | 0.1193 | Tier II |
25 | NC_000009.12 | 9 | 122745106 | - | OR1L6 | NNNTAGATATTAGCAACATG | NGA | 2 | 0.0453 | Tier II |
26 | NC_000012.12 | 12 | 39414855 | + | KIF21A | NNNTAGATATTAATAACATG | NTG | 2 | 0.0286 | Tier II |
27 | NC_000005.10 | 5 | 126621873 | + | PHAX | NNNAAGATATTAACAACATG | NTG | 2 | 0.0186 | Tier II |
28 | NC_000003.12 | 3 | 169508471 | - | MECOM | NNNTAGATATTAACATCCTG | NGG | 2 | 0.0 | Tier II |
29 | NC_000004.12 | 4 | 61532978 | + | ADGRL3 | NNNTAGATATTAAGAACATG | NGA | 2 | 0.0 | Tier II |
30 | NC_000007.14 | 7 | 14819776 | - | DGKB | NNNTAGATATTAAGAACATG | NGC | 2 | 0.0 | Tier II |
31 | NC_000002.12 | 2 | 114891351 | - | DPP10 | NNNTAGATATTAAGTACATG | NGG | 2 | 0.0 | Tier II |
32 | NC_000001.11 | 1 | 194253974 | + | LOC107985242 | NNNTAAATATTAACAATATG | NGG | 2 | 0.4667 | Tier III |
33 | NC_000013.11 | 13 | 92672185 | - | LOC105370315 | NNNTAGAGATTAACAAAATG | NGG | 2 | 0.3422 | Tier III |
34 | NC_000014.9 | 14 | 52902452 | + | LOC105370500 | NNNTAGATATTAACAACAGA | NGG | 2 | 0.2344 | Tier III |
35 | NC_000003.12 | 3 | 30380198 | - | LOC101927995 | NNNTAGATATTAATAACAAG | NGG | 2 | 0.2095 | Tier III |
36 | NC_000009.12 | 9 | 99688267 | - | LOC101928438 | NNNTAGATACTAAAAACATG | NGG | 2 | 0.1867 | Tier III |
37 | NC_000009.12 | 9 | 99688267 | - | LOC124902234 | NNNTAGATACTAAAAACATG | NGG | 2 | 0.1867 | Tier III |
38 | NC_000011.10 | 11 | 91847604 | + | LINC02756 | NNNTAGATATGAACAACCTG | NGG | 2 | 0.0762 | Tier III |
39 | NC_000016.10 | 16 | 63341688 | + | LOC105371308 | NNNTAGATATTAACAACAAG | NAG | 2 | 0.0741 | Tier III |
40 | NC_000016.10 | 16 | 61434631 | + | LOC105371302 | NNNTTGATATTAACTACATG | NGG | 2 | 0.0727 | Tier III |
41 | NC_000016.10 | 16 | 80360627 | - | DYNLRB2-AS1 | NNNTAGAGATTAACAACATG | NGT | 2 | 0.0118 | Tier III |
42 | NC_000013.11 | 13 | 110124182 | + | LINC03082 | NNNTAGATATTAAAAACATG | NGC | 2 | 0.0078 | Tier III |
43 | NC_000013.11 | 13 | 110124182 | + | LINC03032 | NNNTAGATATTAAAAACATG | NGC | 2 | 0.0078 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000081.6 | 15 | 67106511 | + | St3gal1 | NNNTAGATGATAACAACATG | NGG | 2 | 0.5714 | Tier II |
2 | NC_000067.6 | 1 | 37655953 | + | Cracdl | NNNTAGATTTTAATAACATG | NGG | 2 | 0.44 | Tier II |
3 | NC_000067.6 | 1 | 177824827 | - | Catspere1 | NNNTAGATAATAACAACATG | NAG | 2 | 0.2222 | Tier II |
4 | NC_000076.6 | 10 | 12645282 | + | Utrn | NNNTAGATATTCACAACATG | NGC | 2 | 0.0058 | Tier II |
5 | NC_000069.6 | 3 | 18194245 | + | Cyp7b1 | NNNTAGATATTAACAAGATG | NGT | 2 | 0.0009 | Tier II |
6 | NC_000077.6 | 11 | 34730839 | - | Dock2 | NNNTAGATATTAACACCATG | NGG | 1 | 0.0 | Tier II |
7 | NC_000070.6 | 4 | 46760564 | - | Gabbr2 | NNNTGGATATTAACACCATG | NGG | 2 | 0.0 | Tier II |
8 | NC_000072.6 | 6 | 17163188 | - | Gm4876 | NNNTACATATTAATAACATG | NGG | 2 | 0.5 | Tier III |
9 | NC_000067.6 | 1 | 37655953 | + | 4930470B04Rik | NNNTAGATTTTAATAACATG | NGG | 2 | 0.44 | Tier III |
10 | NC_000076.6 | 10 | 84081841 | - | Gm34184 | NNNTAGATTTTAACCACATG | NGG | 2 | 0.1636 | Tier III |
11 | NC_000081.6 | 15 | 62669953 | + | Gm41333 | NNNTAGATATTACCATCATG | NGG | 2 | 0.0 | Tier III |