Construct: sgRNA BRDN0001147425

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGGCTAACTGTGATCTCAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLK1 (9874)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75995

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 171050160 - TLK1 NNNCTAACTGTGATCTCAGA NGG 0 1.0 Tier I
2 NC_000004.12 4 110517700 + ENPEP NNNTTAACTGTGATCACAGA NGG 2 0.7273 Tier II
3 NC_000018.10 18 79339213 - ATP9B NNNCCTACTGTGATCTCAGA NGG 2 0.7143 Tier II
4 NC_000001.11 1 50646834 + FAF1 NNNCTAAATATGATCTCAGA NGG 2 0.6067 Tier II
5 NC_000008.11 8 30790311 + PPP2CB NNNCTGACTGTGATCCCAGA NGG 2 0.4762 Tier II
6 NC_000001.11 1 62045595 + PATJ NNNCTGACTGTGATCTCATA NGG 2 0.4762 Tier II
7 NC_000006.12 6 65189685 - EYS NNNCCAACTGTGATCTAAGA NGG 2 0.4667 Tier II
8 NC_000001.11 1 119992997 + NOTCH2 NNNCTAACTGTGAACTCATA NGG 2 0.4127 Tier II
9 NC_000001.11 1 120797477 - NBPF26 NNNCTAACTGTGAACTCATA NGG 2 0.4127 Tier II
10 NC_000001.11 1 146152482 + NOTCH2NLA NNNCTAACTGTGAACTCATA NGG 2 0.4127 Tier II
11 NC_000001.11 1 148603424 + NOTCH2NLB NNNCTAACTGTGAACTCATA NGG 2 0.4127 Tier II
12 NC_000001.11 1 149467816 - NOTCH2NLC NNNCTAACTGTGAACTCATA NGG 2 0.4127 Tier II
13 NC_000020.11 20 19440339 + SLC24A3 NNNCTATCTGTAATCTCAGA NGG 2 0.4083 Tier II
14 NC_000012.12 12 119084173 - SRRM4 NNNCAGACTGTGATCTCAGA NGG 2 0.3571 Tier II
15 NC_000002.12 2 143750592 - ARHGAP15 NNNCTGACTGTGATCTTAGA NGG 2 0.3333 Tier II
16 NC_000021.9 21 31964555 + HUNK NNNCTAAGTGTTATCTCAGA NGG 2 0.2473 Tier II
17 NC_000022.11 22 44203499 - PARVG NNNCAAACTGTGATCTAAGA NGG 2 0.2333 Tier II
18 NC_000018.10 18 53663516 + LOC124904304 NNNCTCACTGTGATCTCACA NGG 2 0.2038 Tier II
19 NC_000019.10 19 45349087 - KLC3 NNNCAAACTGGGATCTCAGA NGG 2 0.2 Tier II
20 NC_000007.14 7 137699922 + DGKI NNNCTATCTGTGTTCTCAGA NGG 2 0.1313 Tier II
21 NC_000006.12 6 119137246 + FAM184A NNNCTAACTGTGACCTCACA NGG 2 0.1281 Tier II
22 NC_000003.12 3 61578673 - PTPRG NNNCTAACTGTTTTCTCAGA NGG 2 0.1154 Tier II
23 NC_000011.10 11 22853093 - CCDC179 NNNGTAACTGTGATATCAGA NGG 2 0.1111 Tier II
24 NC_000002.12 2 17578623 + VSNL1 NNNCTACCTGTGCTCTCAGA NGG 2 0.0921 Tier II
25 NC_000011.10 11 94777119 - AMOTL1 NNNCTTACTGTGATTTCAGA NGG 2 0.0476 Tier II
26 NC_000021.9 21 45105549 - ADARB1 NNNTTAACTGTGATCTGAGA NGG 2 0.0471 Tier II
27 NC_000002.12 2 214011975 + SPAG16 NNNCTAACTTTGATCTCAGA NTG 2 0.0317 Tier II
28 NC_000015.10 15 74124109 + ISLR2 NNNCTGACTGTGATCTCAGA NTG 2 0.0278 Tier II
29 NC_000003.12 3 39091158 + WDR48 NNNCTCACTGTGATGTCAGA NGG 2 0.0227 Tier II
30 NC_000009.12 9 122686860 + TLK1P1 NNNCTAACCGTGATCTCAGA NGG 1 0.9231 Tier III
31 NC_000014.9 14 49993533 - LINC01588 NNNCTGAATGTGATCTCAGA NGG 2 0.4643 Tier III
32 NC_000002.12 2 143750592 - ARHGAP15-AS1 NNNCTGACTGTGATCTTAGA NGG 2 0.3333 Tier III
33 NC_000008.11 8 106031331 + ZFPM2-AS1 NNNCTAACTATGATATCAGA NGG 2 0.2074 Tier III
34 NC_000004.12 4 144334433 + LOC105377462 NNNCTCACTGTGATCTCACA NGG 2 0.2038 Tier III
35 NC_000012.12 12 91353203 + LOC105369896 NNNCTCACTGTGTTCTCAGA NGG 2 0.1364 Tier III
36 NC_000012.12 12 91353203 + LINC02823 NNNCTCACTGTGTTCTCAGA NGG 2 0.1364 Tier III
37 NC_000011.10 11 22853093 - LINC02718 NNNGTAACTGTGATATCAGA NGG 2 0.1111 Tier III
38 NC_000018.10 18 52099288 + LOC105372121 NNNCAAACTGTGCTCTCAGA NGG 2 0.1053 Tier III
39 NC_000009.12 9 86685473 - LINC02834 NNNCTATCTGTGATCTGAGA NGG 2 0.0257 Tier III
40 NC_000017.11 17 13826946 + LOC100506974 NNNCTAACTGTAAGCTCAGA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 70746030 - Tlk1 NNNCCAACTGTGATCTCAGG NGG 2 0.7647 Tier I
2 NC_000069.6 3 115088028 - Olfm3 NNNCCAACTGTGATCTCAGA NGG 1 1.0 Tier II
3 NC_000080.6 14 57355642 + Cryl1 NNNCCAACTGAGATCTCAGA NGG 2 0.75 Tier II
4 NC_000077.6 11 22038994 + Ehbp1 NNNCTGACTGTGATCTCAGA NGG 1 0.7143 Tier II
5 NC_000085.6 19 17014822 - Prune2 NNNCTAACAGTGGTCTCAGA NGG 2 0.6056 Tier II
6 NC_000077.6 11 116613075 - Rhbdf2 NNNCCATCTGTGATCTCAGA NGG 2 0.4375 Tier II
7 NC_000080.6 14 123697050 - Itgbl1 NNNCTGACTGTCATCTCAGA NGG 2 0.3782 Tier II
8 NC_000071.6 5 136789657 - Col26a1 NNNTTAACTCTGATCTCAGA NGG 2 0.32 Tier II
9 NC_000077.6 11 47881477 + Sgcd NNNCTAAGTGTGATCTTAGA NGG 2 0.3 Tier II
10 NC_000079.6 13 24238292 - Carmil1 NNNCTAACTGGGATCCCAGA NGG 2 0.2667 Tier II
11 NC_000067.6 1 151670014 - Niban1 NNNCTACCTGTGATCTCAGT NGG 2 0.2625 Tier II
12 NC_000074.6 8 32339520 - Nrg1 NNNCTAACTTTGTTCTCAGA NGG 2 0.2438 Tier II
13 NC_000075.6 9 14285766 + Sesn3 NNNCTAACTGGCATCTCAGA NGG 2 0.2118 Tier II
14 NC_000076.6 10 82366351 - Gm4924 NNNCTAACTGTACTCTCAGA NGG 2 0.1965 Tier II
15 NC_000084.6 18 57539130 - Ccdc192 NNNCTAACTGAGATCTCAGA NAG 2 0.1944 Tier II
16 NC_000074.6 8 36492356 + Trmt9b NNNCCAACTGTGATGTCAGA NGG 2 0.05 Tier II
17 NC_000081.6 15 66688034 + Tg NNNCTAAATGTGATCTCAGA NGA 2 0.0451 Tier II
18 NC_000080.6 14 12589356 - Cadps NNNCTAAATGTGATCTCAGA NTG 2 0.0253 Tier II
19 NC_000077.6 11 68716604 - Myh10 NNNCTAACTGTGCTCTCAGA NGA 2 0.0146 Tier II
20 NC_000077.6 11 106515281 - Tex2 NNNCTCACTGTGATCTCAGA NGC 2 0.0101 Tier II
21 NC_000067.6 1 90560479 + Gm41917 NNNCTTACTGTGATCACAGA NGG 2 0.6494 Tier III
22 NC_000068.7 2 163143240 + Gm11454 NNNTTAACTGTCATCTCAGA NGG 2 0.4235 Tier III
23 NC_000083.6 17 84143020 + Gm19696 NNNCTAACAGTGCTCTCAGA NGG 2 0.1955 Tier III
24 NC_000070.6 4 52433017 + Smc2os NNNCTACCTCTGATCTCAGA NGG 2 0.175 Tier III
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Other clones with same target sequence:

(none)