Construct: sgRNA BRDN0001147431
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGACCTTAAGCCTCCACAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HIPK1 (204851)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76994
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
113954758 |
- |
HIPK1 |
NNNCCTTAAGCCTCCACAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
27369458 |
- |
FCN3 |
NNNCCCTGAGCCTCCACAGT |
NGG |
2 |
0.6667 |
Tier I |
| 3 |
NC_000016.10 |
16 |
89967429 |
- |
DEF8 |
NNNCCTAAAGCCACCACAGT |
NGG |
2 |
0.6058 |
Tier II |
| 4 |
NC_000005.10 |
5 |
108161017 |
+ |
FBXL17 |
NNNCTTTAAGCCTCCACATT |
NGG |
2 |
0.4242 |
Tier II |
| 5 |
NC_000020.11 |
20 |
5996167 |
- |
MCM8 |
NNNACTTCAGCCTCCACAGT |
NGG |
2 |
0.3609 |
Tier II |
| 6 |
NC_000003.12 |
3 |
51358766 |
+ |
DOCK3 |
NNNTCTTCAGCCTCCACAGT |
NGG |
2 |
0.3429 |
Tier II |
| 7 |
NC_000018.10 |
18 |
13353327 |
- |
LDLRAD4 |
NNNCCTTAAGCCTTCACAGT |
NAG |
2 |
0.1901 |
Tier II |
| 8 |
NC_000009.12 |
9 |
15833669 |
- |
CCDC171 |
NNNCCTTAAGCCTCCACATT |
NGA |
2 |
0.0463 |
Tier II |
| 9 |
NC_000021.9 |
21 |
45083673 |
- |
ADARB1 |
NNNCTTTAAGCCTCCACAGT |
NGA |
2 |
0.0442 |
Tier II |
| 10 |
NC_000009.12 |
9 |
69581885 |
- |
APBA1 |
NNNCCTTCAGCCTCCACAGT |
NTG |
2 |
0.0167 |
Tier II |
| 11 |
NC_000017.11 |
17 |
73214219 |
- |
VCF1 |
NNNCCCTAAGCCTCCACAGT |
NGT |
2 |
0.0147 |
Tier II |
| 12 |
NC_000022.11 |
22 |
17592321 |
+ |
ATP6V1E1 |
NNNCCTTAAGCCTCAAGAGT |
NGG |
2 |
0.0131 |
Tier II |
| 13 |
NC_000006.12 |
6 |
43559943 |
+ |
POLR1C |
NNNCCTTAAGCCTCCCCAGT |
NGC |
2 |
0.0 |
Tier II |
| 14 |
NC_000006.12 |
6 |
43559943 |
+ |
XPO5 |
NNNCCTTAAGCCTCCCCAGT |
NGC |
2 |
0.0 |
Tier II |
| 15 |
NC_000005.10 |
5 |
96403077 |
- |
CAST |
NNNCCTTAAGCCTCCTCAGT |
NCG |
2 |
0.0 |
Tier II |
| 16 |
NC_000005.10 |
5 |
96403077 |
- |
PCSK1 |
NNNCCTTAAGCCTCCTCAGT |
NCG |
2 |
0.0 |
Tier II |
| 17 |
NC_000023.11 |
X |
9832245 |
+ |
SHROOM2 |
NNNCCTTAGGCCTGCACAGT |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000017.11 |
17 |
20615478 |
- |
LINC02088 |
NNNCCTAAAGCCACCACAGT |
NGG |
2 |
0.6058 |
Tier III |
| 19 |
NC_000020.11 |
20 |
5996167 |
- |
MCM8-AS1 |
NNNACTTCAGCCTCCACAGT |
NGG |
2 |
0.3609 |
Tier III |
| 20 |
NC_000004.12 |
4 |
124196674 |
+ |
LOC105377406 |
NNNCCTTAAGCAGCCACAGT |
NGG |
2 |
0.1863 |
Tier III |
| 21 |
NC_000004.12 |
4 |
124196674 |
+ |
LOC105377407 |
NNNCCTTAAGCAGCCACAGT |
NGG |
2 |
0.1863 |
Tier III |
| 22 |
NC_000005.10 |
5 |
96403077 |
- |
LOC101929710 |
NNNCCTTAAGCCTCCTCAGT |
NCG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
103764475 |
+ |
Hipk1 |
NNNCCTTAAGCCTCCACAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000074.6 |
8 |
110816884 |
- |
Sf3b3 |
NNNCCTTGAGCCTCCACAGT |
NGG |
1 |
0.7333 |
Tier II |
| 3 |
NC_000071.6 |
5 |
93652908 |
- |
Pramel34 |
NNNCCCTGAGCCTCCACAGT |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000071.6 |
5 |
95332819 |
+ |
Pramel47 |
NNNCCCTGAGCCTCCACAGT |
NGG |
2 |
0.6667 |
Tier II |
| 5 |
NT_187059.1 |
5 |
614486 |
+ |
LOC100041806 |
NNNCCCTGAGCCTCCACAGT |
NGG |
2 |
0.6667 |
Tier II |
| 6 |
NC_000067.6 |
1 |
185290765 |
- |
Iars2 |
NNNCCTTAAACATCCACAGT |
NGG |
2 |
0.6667 |
Tier II |
| 7 |
NC_000081.6 |
15 |
67150476 |
+ |
St3gal1 |
NNNCCTTAAACCACCACAGT |
NGG |
2 |
0.6462 |
Tier II |
| 8 |
NC_000068.7 |
2 |
73770066 |
+ |
Chn1 |
NNNCTTTAAACCTCCACAGT |
NGG |
2 |
0.5939 |
Tier II |
| 9 |
NC_000071.6 |
5 |
112308751 |
+ |
Tpst2 |
NNNCCTTCAGCCTCCACAGT |
NGG |
1 |
0.4286 |
Tier II |
| 10 |
NC_000068.7 |
2 |
168525282 |
+ |
Nfatc2 |
NNNCCTTAGGCTTCCACAGT |
NGG |
2 |
0.359 |
Tier II |
| 11 |
NC_000086.7 |
X |
82956786 |
+ |
Dmd |
NNNCCTTAAGCTGCCACAGT |
NGG |
2 |
0.1405 |
Tier II |
| 12 |
NC_000074.6 |
8 |
23074039 |
+ |
Ank1 |
NNNCCTGAAGCCTCCGCAGT |
NGG |
2 |
0.1131 |
Tier II |
| 13 |
NC_000084.6 |
18 |
7483536 |
+ |
Mpp7 |
NNNCCTTTAGCCTCCACAGT |
NAG |
2 |
0.1111 |
Tier II |
| 14 |
NC_000074.6 |
8 |
108874418 |
+ |
Zfhx3 |
NNNCCTTAAGGCTCAACAGT |
NGG |
2 |
0.0556 |
Tier II |
| 15 |
NC_000067.6 |
1 |
89607943 |
- |
Agap1 |
NNNCCTTGAGCCTCCACAGT |
NTG |
2 |
0.0286 |
Tier II |
| 16 |
NC_000077.6 |
11 |
32194046 |
+ |
Il9r |
NNNCCTTAAGCCTCCACAGC |
NAG |
2 |
0.0236 |
Tier II |
| 17 |
NC_000078.6 |
12 |
29661773 |
- |
Myt1l |
NNNCCTTAAGTCTCCACAGT |
NGA |
2 |
0.0214 |
Tier II |
| 18 |
NC_000072.6 |
6 |
45509419 |
- |
Cntnap2 |
NNNCCTTAAGCTTCCACAGT |
NTG |
2 |
0.021 |
Tier II |
| 19 |
NC_000075.6 |
9 |
95718355 |
+ |
Trpc1 |
NNNCCTCAAGCCTGCACAGT |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000071.6 |
5 |
94675267 |
+ |
Gm6205 |
NNNCCCTGAGCCTCCACAGT |
NGG |
2 |
0.6667 |
Tier III |
| 21 |
NC_000071.6 |
5 |
95015503 |
+ |
Gm6367 |
NNNCCCTGAGCCTCCACAGT |
NGG |
2 |
0.6667 |
Tier III |
| 22 |
NT_187059.1 |
5 |
300566 |
+ |
LOC100041672 |
NNNCCCTGAGCCTCCACAGT |
NGG |
2 |
0.6667 |
Tier III |
| 23 |
NC_000071.6 |
5 |
30578886 |
- |
Gm9899 |
NNNCCTTGAGCATCCACAGT |
NGG |
2 |
0.5238 |
Tier III |
| 24 |
NC_000084.6 |
18 |
54820533 |
- |
Gm33732 |
NNNCCTTAAGCATCCTCAGT |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
