Construct: sgRNA BRDN0001147466

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CACTAATGTGACAACAGCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NRBP1 (29959)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77017

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 27437068 + NRBP1 NNNTAATGTGACAACAGCAG NGG 0 1.0 Tier I
2 NC_000001.11 1 26779552 + ARID1A NNNGCATGTGACAACAGCAG NGG 2 0.3125 Tier I
3 NC_000016.10 16 71924867 - IST1 NNNTAATGAGAAAACAGCAG NGG 2 0.6633 Tier II
4 NC_000010.11 10 113062271 - TCF7L2 NNNTGATGTGGCAACAGCAG NGG 2 0.468 Tier II
5 NC_000011.10 11 83989282 + DLG2 NNNCAATGGGACAACAGCAG NGG 2 0.4006 Tier II
6 NC_000023.11 X 10135609 - WWC3 NNNCAATGGGACAACAGCAG NGG 2 0.4006 Tier II
7 NC_000019.10 19 45746443 - MEIOSIN NNNCAATGTGACAACAGAAG NGG 2 0.3484 Tier II
8 NC_000002.12 2 199381329 + SATB2 NNNAAATGTGAAAACAGCAG NGG 2 0.3401 Tier II
9 NC_000013.11 13 45018067 - GPALPP1 NNNTAATGTGAGAACAGTAG NGG 2 0.2857 Tier II
10 NC_000007.14 7 139591568 + HIPK2 NNNTAATGTGACAGCAGCTG NGG 2 0.2513 Tier II
11 NC_000002.12 2 96504438 + NEURL3 NNNTACTGTGACAGCAGCAG NGG 2 0.2121 Tier II
12 NC_000018.10 18 79936124 - SLC66A2 NNNTCATGTGCCAACAGCAG NGG 2 0.2 Tier II
13 NC_000003.12 3 140547959 + CLSTN2 NNNGAATGTGACAAAAGCAG NGG 2 0.1389 Tier II
14 NC_000007.14 7 8712119 + NXPH1 NNNTAATGTGACTACAGCAC NGG 2 0.1286 Tier II
15 NC_000007.14 7 18153265 + HDAC9 NNNTAAAGTGACAACAGCAG NGA 2 0.0608 Tier II
16 NC_000015.10 15 47654740 - SEMA6D NNNTAATGTGACAAAAGCAG NAG 2 0.0576 Tier II
17 NC_000011.10 11 24848430 - LUZP2 NNNTAATGAGACAAGAGCAG NGG 2 0.0464 Tier II
18 NC_000003.12 3 33316563 - FBXL2 NNNTAATGTGAGAACAGCAG NTG 2 0.0173 Tier II
19 NC_000009.12 9 17957508 - ADAMTSL1 NNNTAATGTGAGAACAGCAG NTG 2 0.0173 Tier II
20 NC_000002.12 2 114596836 - DPP10 NNNTAATGTAACAACAGCAG NGT 2 0.0151 Tier II
21 NC_000006.12 6 38271901 + BTBD9 NNNTACTGTGACAACTGCAG NGG 2 0.0 Tier II
22 NC_000014.9 14 68987236 - ACTN1-DT NNNTAATGAGAAAACAGCAG NGG 2 0.6633 Tier III
23 NC_000008.11 8 141725882 - LOC124902074 NNNGAATGTGGCAACAGCAG NGG 2 0.4063 Tier III
24 NC_000018.10 18 42687483 + LINC00907 NNNAAATGTGAAAACAGCAG NGG 2 0.3401 Tier III
25 NC_000008.11 8 128052743 - PVT1 NNNTAGTGTGACAACAGCAC NGG 2 0.3061 Tier III
26 NC_000013.11 13 84901077 + LOC105370290 NNNTATTGTGACAACAGCAC NGG 2 0.3061 Tier III
27 NC_000012.12 12 127618635 - LOC124903051 NNNTTATGTTACAACAGCAG NGG 2 0.2955 Tier III
28 NC_000010.11 10 84001380 - LOC105378399 NNNTCAGGTGACAACAGCAG NGG 2 0.2941 Tier III
29 NC_000007.14 7 139591568 + LOC105375530 NNNTAATGTGACAGCAGCTG NGG 2 0.2513 Tier III
30 NC_000012.12 12 92602399 - LOC105369905 NNNTAATGTGACAAAAGCAG NGG 1 0.2222 Tier III
31 NC_000012.12 12 92602399 - LOC124902983 NNNTAATGTGACAAAAGCAG NGG 1 0.2222 Tier III
32 NC_000003.12 3 63116764 + LINC00698 NNNTACTGTGAGAACAGCAG NGG 2 0.202 Tier III
33 NC_000012.12 12 15294910 + LOC105369673 NNNTAATGAGACACCAGCAG NGG 2 0.199 Tier III
34 NC_000006.12 6 133814631 - TARID NNNTTATGTGACATCAGCAG NGG 2 0.1939 Tier III
35 NC_000009.12 9 103281162 - LINC01492 NNNTAATGTGACAACAGCAA NGA 2 0.0651 Tier III
36 NC_000015.10 15 92896075 - CHASERR NNNTTATGTGACAACAGCAG NGA 2 0.0253 Tier III
37 NC_000021.9 21 6875016 - LOC110091777 NNNTAATGTGACAAAAGCAG NGT 2 0.0036 Tier III
38 NC_000021.9 21 13740065 - LOC112268283 NNNTAATGTGACAAAAGCAG NGT 2 0.0036 Tier III
39 NT_167208.1 9522 + LOC101927555 NNNTAATGTGACAAAAGCAG NGT 2 0.0036 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 31247705 + Nrbp1 NNNAAACGTGACAACAGCAG NGG 2 0.3274 Tier I
2 NC_000085.6 19 5892493 - Tigd3 NNNTTATGTGCCAACAGCAG NGG 2 0.1455 Tier I
3 NC_000082.6 16 11415875 - Snx29 NNNTAATGTAACAACAGCAA NGG 2 0.875 Tier II
4 NC_000070.6 4 32344709 + Bach2 NNNAAATGTAACAACAGCAG NGG 2 0.4444 Tier II
5 NC_000068.7 2 156869082 - Rab5if NNNTCACGTGACAACAGCAG NGG 2 0.3438 Tier II
6 NC_000070.6 4 84592612 - Bnc2 NNNAAATTTGACAACAGCAG NGG 2 0.2976 Tier II
7 NC_000068.7 2 91959182 - Dgkz NNNCACTGTGACAACAGCAG NGG 2 0.2941 Tier II
8 NC_000074.6 8 47255864 - Stox2 NNNTAATGTGTAAACAGCAG NGG 2 0.2198 Tier II
9 NC_000076.6 10 53712531 + Fam184a NNNTAATGTCACAACACCAG NGG 2 0.0941 Tier II
10 NC_000072.6 6 72672840 - Tcf7l1 NNNTAATGTGACAATAGCAT NGG 2 0.0467 Tier II
11 NC_000074.6 8 67719388 + Psd3 NNNTAATCTGACAACAGCAG NGA 2 0.0427 Tier II
12 NC_000081.6 15 18821754 - Cdh10 NNNTAATGTGACATCAGCAG NGT 2 0.0086 Tier II
13 NC_000082.6 16 10204504 - Atf7ip2 NNNTAATGTGACAACAGCAG NAC 2 0.0 Tier II
14 NC_000077.6 11 59937276 - Gm51900 NNNTGATGTAACAACAGCAG NGG 2 0.672 Tier III
15 NC_000078.6 12 80272162 - Gm29952 NNNTAATGAGAAAACAGCAG NGG 2 0.6633 Tier III
16 NC_000074.6 8 107780619 - Gm42040 NNNTAATGTGACAGCAGCAG NGG 1 0.4667 Tier III
17 NC_000072.6 6 32639175 + Gm32007 NNNAAAAGTGACAACAGCAG NGG 2 0.4167 Tier III
18 NC_000076.6 10 43793847 + Gm40635 NNNTAATGTGGCAGCAGCAG NGG 2 0.3033 Tier III
19 NC_000072.6 6 72672840 - Gm31391 NNNTAATGTGACAATAGCAT NGG 2 0.0467 Tier III
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Other clones with same target sequence:

(none)